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Detection of Porcine Circovirus 3 in Wildlife Species in Spain
Porcine circovirus 3 (PCV-3) is the third member of the family Circoviridae, genus Circovirus, able to infect swine. A high prevalence of viral DNA has been recorded in wild boars. Recently, PCV-3 DNA was identified in Italian wild ruminants. Based on these previous results, this study assessed the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281317/ https://www.ncbi.nlm.nih.gov/pubmed/32369945 http://dx.doi.org/10.3390/pathogens9050341 |
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author | Czyżewska-Dors, Ewelina Núñez, José I. Saporiti, Viviane Huerta, Eva Riutord, Carme Cabezón, Oscar Segalés, Joaquim Sibila, Marina |
author_facet | Czyżewska-Dors, Ewelina Núñez, José I. Saporiti, Viviane Huerta, Eva Riutord, Carme Cabezón, Oscar Segalés, Joaquim Sibila, Marina |
author_sort | Czyżewska-Dors, Ewelina |
collection | PubMed |
description | Porcine circovirus 3 (PCV-3) is the third member of the family Circoviridae, genus Circovirus, able to infect swine. A high prevalence of viral DNA has been recorded in wild boars. Recently, PCV-3 DNA was identified in Italian wild ruminants. Based on these previous results, this study assessed the frequency of PCV-3 DNA detection in free-ranging ruminants and Lagomorpha species in Spain. In addition, the genetic characterization of the PCV-3 PCR-positive samples was performed. A total of 801 serum samples, including red deer (Cervus elaphus, [CE]; n = 108), roe deer (Capreolus capreolus, [CC]; n = 87), Pyrenean chamois (Rupicapra pyrenaica, [RP]; n = 133), Iberian ibex (Capra pyrenaica, [CP]; n = 92), mouflon (Ovis aries, [OA]; n = 91), fallow deer (Dama dama, [DD]; n = 104), European rabbit (Oryctolagus cuniculus, [OC]; n = 101), and European hare (Lepus europaeus, [LE]; n = 85) from Catalonia (northeast Spain) were tested by conventional polymerase chain reaction (PCR) and, when positive, sequenced. Overall, PCV-3 DNA was found in three out of 801 analyzed sera (0.37%) corresponding to one red deer (1/108, 0.9%), one mouflon (1/91, 1.1%), and one fallow deer (1/104, 0.96%). None of the samples collected from Lagomorpha species resulted PCR positive. The partial genome sequences detected in positive samples displayed high identity with some PCV-3 sequences detected in wild boars and domestic pigs (99.7% and 100%, respectively). In conclusion, the present study indicated that free-ranging ruminant and Lagomorpha species are not relevant in the epidemiology of PCV-3 in Spain. |
format | Online Article Text |
id | pubmed-7281317 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72813172020-06-19 Detection of Porcine Circovirus 3 in Wildlife Species in Spain Czyżewska-Dors, Ewelina Núñez, José I. Saporiti, Viviane Huerta, Eva Riutord, Carme Cabezón, Oscar Segalés, Joaquim Sibila, Marina Pathogens Communication Porcine circovirus 3 (PCV-3) is the third member of the family Circoviridae, genus Circovirus, able to infect swine. A high prevalence of viral DNA has been recorded in wild boars. Recently, PCV-3 DNA was identified in Italian wild ruminants. Based on these previous results, this study assessed the frequency of PCV-3 DNA detection in free-ranging ruminants and Lagomorpha species in Spain. In addition, the genetic characterization of the PCV-3 PCR-positive samples was performed. A total of 801 serum samples, including red deer (Cervus elaphus, [CE]; n = 108), roe deer (Capreolus capreolus, [CC]; n = 87), Pyrenean chamois (Rupicapra pyrenaica, [RP]; n = 133), Iberian ibex (Capra pyrenaica, [CP]; n = 92), mouflon (Ovis aries, [OA]; n = 91), fallow deer (Dama dama, [DD]; n = 104), European rabbit (Oryctolagus cuniculus, [OC]; n = 101), and European hare (Lepus europaeus, [LE]; n = 85) from Catalonia (northeast Spain) were tested by conventional polymerase chain reaction (PCR) and, when positive, sequenced. Overall, PCV-3 DNA was found in three out of 801 analyzed sera (0.37%) corresponding to one red deer (1/108, 0.9%), one mouflon (1/91, 1.1%), and one fallow deer (1/104, 0.96%). None of the samples collected from Lagomorpha species resulted PCR positive. The partial genome sequences detected in positive samples displayed high identity with some PCV-3 sequences detected in wild boars and domestic pigs (99.7% and 100%, respectively). In conclusion, the present study indicated that free-ranging ruminant and Lagomorpha species are not relevant in the epidemiology of PCV-3 in Spain. MDPI 2020-05-01 /pmc/articles/PMC7281317/ /pubmed/32369945 http://dx.doi.org/10.3390/pathogens9050341 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Czyżewska-Dors, Ewelina Núñez, José I. Saporiti, Viviane Huerta, Eva Riutord, Carme Cabezón, Oscar Segalés, Joaquim Sibila, Marina Detection of Porcine Circovirus 3 in Wildlife Species in Spain |
title | Detection of Porcine Circovirus 3 in Wildlife Species in Spain |
title_full | Detection of Porcine Circovirus 3 in Wildlife Species in Spain |
title_fullStr | Detection of Porcine Circovirus 3 in Wildlife Species in Spain |
title_full_unstemmed | Detection of Porcine Circovirus 3 in Wildlife Species in Spain |
title_short | Detection of Porcine Circovirus 3 in Wildlife Species in Spain |
title_sort | detection of porcine circovirus 3 in wildlife species in spain |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281317/ https://www.ncbi.nlm.nih.gov/pubmed/32369945 http://dx.doi.org/10.3390/pathogens9050341 |
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