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Microelectrode Array based Functional Testing of Pancreatic Islet Cells

Electrophysiological techniques to characterize the functionality of islets of Langerhans have been limited to short-term, one-time recordings such as a patch clamp recording. We describe the use of microelectrode arrays (MEAs) to better understand the electrophysiology of dissociated islet cells in...

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Autores principales: Alassaf, Ahmad, Ishahak, Matthew, Bowles, Annie, Agarwal, Ashutosh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281363/
https://www.ncbi.nlm.nih.gov/pubmed/32429597
http://dx.doi.org/10.3390/mi11050507
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author Alassaf, Ahmad
Ishahak, Matthew
Bowles, Annie
Agarwal, Ashutosh
author_facet Alassaf, Ahmad
Ishahak, Matthew
Bowles, Annie
Agarwal, Ashutosh
author_sort Alassaf, Ahmad
collection PubMed
description Electrophysiological techniques to characterize the functionality of islets of Langerhans have been limited to short-term, one-time recordings such as a patch clamp recording. We describe the use of microelectrode arrays (MEAs) to better understand the electrophysiology of dissociated islet cells in response to glucose in a real-time, non-invasive method over prolonged culture periods. Human islets were dissociated into singular cells and seeded onto MEA, which were cultured for up to 7 days. Immunofluorescent imaging revealed that several cellular subtypes of islets; β, δ, and γ cells were present after dissociation. At days 1, 3, 5, and 7 of culture, MEA recordings captured higher electrical activities of islet cells under 16.7 mM glucose (high glucose) than 1.1 mM glucose (low glucose) conditions. The fraction of the plateau phase (FOPP), which is the fraction of time with spiking activity recorded using the MEA, consistently showed distinguishably greater percentages of spiking activity with high glucose compared to the low glucose for all culture days. In parallel, glucose stimulated insulin secretion was measured revealing a diminished insulin response after day 3 of culture. Additionally, MEA spiking profiles were similar to the time course of insulin response when glucose concentration is switched from 1.1 to 16.7 mM. Our analyses suggest that extracellular recordings of dissociated islet cells using MEA is an effective approach to rapidly assess islet functionality, and could supplement standard assays such as glucose stimulate insulin response.
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spelling pubmed-72813632020-06-19 Microelectrode Array based Functional Testing of Pancreatic Islet Cells Alassaf, Ahmad Ishahak, Matthew Bowles, Annie Agarwal, Ashutosh Micromachines (Basel) Article Electrophysiological techniques to characterize the functionality of islets of Langerhans have been limited to short-term, one-time recordings such as a patch clamp recording. We describe the use of microelectrode arrays (MEAs) to better understand the electrophysiology of dissociated islet cells in response to glucose in a real-time, non-invasive method over prolonged culture periods. Human islets were dissociated into singular cells and seeded onto MEA, which were cultured for up to 7 days. Immunofluorescent imaging revealed that several cellular subtypes of islets; β, δ, and γ cells were present after dissociation. At days 1, 3, 5, and 7 of culture, MEA recordings captured higher electrical activities of islet cells under 16.7 mM glucose (high glucose) than 1.1 mM glucose (low glucose) conditions. The fraction of the plateau phase (FOPP), which is the fraction of time with spiking activity recorded using the MEA, consistently showed distinguishably greater percentages of spiking activity with high glucose compared to the low glucose for all culture days. In parallel, glucose stimulated insulin secretion was measured revealing a diminished insulin response after day 3 of culture. Additionally, MEA spiking profiles were similar to the time course of insulin response when glucose concentration is switched from 1.1 to 16.7 mM. Our analyses suggest that extracellular recordings of dissociated islet cells using MEA is an effective approach to rapidly assess islet functionality, and could supplement standard assays such as glucose stimulate insulin response. MDPI 2020-05-17 /pmc/articles/PMC7281363/ /pubmed/32429597 http://dx.doi.org/10.3390/mi11050507 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alassaf, Ahmad
Ishahak, Matthew
Bowles, Annie
Agarwal, Ashutosh
Microelectrode Array based Functional Testing of Pancreatic Islet Cells
title Microelectrode Array based Functional Testing of Pancreatic Islet Cells
title_full Microelectrode Array based Functional Testing of Pancreatic Islet Cells
title_fullStr Microelectrode Array based Functional Testing of Pancreatic Islet Cells
title_full_unstemmed Microelectrode Array based Functional Testing of Pancreatic Islet Cells
title_short Microelectrode Array based Functional Testing of Pancreatic Islet Cells
title_sort microelectrode array based functional testing of pancreatic islet cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281363/
https://www.ncbi.nlm.nih.gov/pubmed/32429597
http://dx.doi.org/10.3390/mi11050507
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