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Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma
Sequencing studies have provided novel insights into the heterogeneous molecular landscape of glioblastoma (GBM), unveiling a subset of patients with gene fusions. Tissue biopsy is highly invasive, limited by sampling frequency and incompletely representative of intra-tumor heterogeneity. Extracellu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281415/ https://www.ncbi.nlm.nih.gov/pubmed/32414213 http://dx.doi.org/10.3390/cancers12051219 |
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author | Wang, Lan Yekula, Anudeep Muralidharan, Koushik Small, Julia L. Rosh, Zachary S. Kang, Keiko M. Carter, Bob S. Balaj, Leonora |
author_facet | Wang, Lan Yekula, Anudeep Muralidharan, Koushik Small, Julia L. Rosh, Zachary S. Kang, Keiko M. Carter, Bob S. Balaj, Leonora |
author_sort | Wang, Lan |
collection | PubMed |
description | Sequencing studies have provided novel insights into the heterogeneous molecular landscape of glioblastoma (GBM), unveiling a subset of patients with gene fusions. Tissue biopsy is highly invasive, limited by sampling frequency and incompletely representative of intra-tumor heterogeneity. Extracellular vesicle-based liquid biopsy provides a minimally invasive alternative to diagnose and monitor tumor-specific molecular aberrations in patient biofluids. Here, we used targeted RNA sequencing to screen GBM tissue and the matched plasma of patients (n = 9) for RNA fusion transcripts. We identified two novel fusion transcripts in GBM tissue and five novel fusions in the matched plasma of GBM patients. The fusion transcripts FGFR3-TACC3 and VTI1A-TCF7L2 were detected in both tissue and matched plasma. A longitudinal follow-up of a GBM patient with a FGFR3-TACC3 positive glioma revealed the potential of monitoring RNA fusions in plasma. In summary, we report a sensitive RNA-seq-based liquid biopsy strategy to detect RNA level fusion status in the plasma of GBM patients. |
format | Online Article Text |
id | pubmed-7281415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72814152020-06-19 Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma Wang, Lan Yekula, Anudeep Muralidharan, Koushik Small, Julia L. Rosh, Zachary S. Kang, Keiko M. Carter, Bob S. Balaj, Leonora Cancers (Basel) Article Sequencing studies have provided novel insights into the heterogeneous molecular landscape of glioblastoma (GBM), unveiling a subset of patients with gene fusions. Tissue biopsy is highly invasive, limited by sampling frequency and incompletely representative of intra-tumor heterogeneity. Extracellular vesicle-based liquid biopsy provides a minimally invasive alternative to diagnose and monitor tumor-specific molecular aberrations in patient biofluids. Here, we used targeted RNA sequencing to screen GBM tissue and the matched plasma of patients (n = 9) for RNA fusion transcripts. We identified two novel fusion transcripts in GBM tissue and five novel fusions in the matched plasma of GBM patients. The fusion transcripts FGFR3-TACC3 and VTI1A-TCF7L2 were detected in both tissue and matched plasma. A longitudinal follow-up of a GBM patient with a FGFR3-TACC3 positive glioma revealed the potential of monitoring RNA fusions in plasma. In summary, we report a sensitive RNA-seq-based liquid biopsy strategy to detect RNA level fusion status in the plasma of GBM patients. MDPI 2020-05-13 /pmc/articles/PMC7281415/ /pubmed/32414213 http://dx.doi.org/10.3390/cancers12051219 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Lan Yekula, Anudeep Muralidharan, Koushik Small, Julia L. Rosh, Zachary S. Kang, Keiko M. Carter, Bob S. Balaj, Leonora Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma |
title | Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma |
title_full | Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma |
title_fullStr | Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma |
title_full_unstemmed | Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma |
title_short | Novel Gene Fusions in Glioblastoma Tumor Tissue and Matched Patient Plasma |
title_sort | novel gene fusions in glioblastoma tumor tissue and matched patient plasma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281415/ https://www.ncbi.nlm.nih.gov/pubmed/32414213 http://dx.doi.org/10.3390/cancers12051219 |
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