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MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles
Titanium dioxide nanoparticles (TiO(2)-NPs) are widely used for biomedical and food applications, the toxicity of TiO(2)-NPs in vivo and in vitro has been elucidated, but the underlying cytotoxicity of TiO(2)-NPs against microRNA remains largely unknown. The purpose of this study was to analyze micr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281448/ https://www.ncbi.nlm.nih.gov/pubmed/32423014 http://dx.doi.org/10.3390/cancers12051236 |
Sumario: | Titanium dioxide nanoparticles (TiO(2)-NPs) are widely used for biomedical and food applications, the toxicity of TiO(2)-NPs in vivo and in vitro has been elucidated, but the underlying cytotoxicity of TiO(2)-NPs against microRNA remains largely unknown. The purpose of this study was to analyze microRNA profiling induced by TiO(2)-NPs against NCM460 and HCT116 cell lines. Comparative analysis identified 34 and 24 microRNAs were significantly altered in the TiO(2)-NPs treated cells at concentrations of 3 μg/mL and 30 μg/mL, respectively. Functional classification demonstrated that a large proportion of genes involved in metabolism, human disease, and environmental information process were significantly upregulated by TiO(2)-NPs. Bioinformatics analysis suggested that microRNA 378 might be an early indicator of cellular response to exogenous stimuli with apoptotic signals. Furthermore, TiO(2)-NPs significantly altered the expression of microRNA 378b and 378g in HCT116 and NCM460 cell lines at different concentrations from 3 to 6 μg/mL. These concentrations elicit high-sensitivity of stimuli response in colon cancer cells when exposed to the slight doses of TiO(2)-NPs. Our study indicated that microRNAs 378b and 378g may play an important role in TiO(2)-NPs-mediated colonic cytotoxicity, which may provide a valuable insight into the molecular mechanisms of potential risks in colitis and colon cancer. |
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