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MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles

Titanium dioxide nanoparticles (TiO(2)-NPs) are widely used for biomedical and food applications, the toxicity of TiO(2)-NPs in vivo and in vitro has been elucidated, but the underlying cytotoxicity of TiO(2)-NPs against microRNA remains largely unknown. The purpose of this study was to analyze micr...

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Autores principales: Li, Wen, Jia, Ming Xi, Deng, Jing, Wang, Jian Hui, Zuberi, Zavuga, Yang, Sheng, Ba, Jie, Chen, Zhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281448/
https://www.ncbi.nlm.nih.gov/pubmed/32423014
http://dx.doi.org/10.3390/cancers12051236
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author Li, Wen
Jia, Ming Xi
Deng, Jing
Wang, Jian Hui
Zuberi, Zavuga
Yang, Sheng
Ba, Jie
Chen, Zhu
author_facet Li, Wen
Jia, Ming Xi
Deng, Jing
Wang, Jian Hui
Zuberi, Zavuga
Yang, Sheng
Ba, Jie
Chen, Zhu
author_sort Li, Wen
collection PubMed
description Titanium dioxide nanoparticles (TiO(2)-NPs) are widely used for biomedical and food applications, the toxicity of TiO(2)-NPs in vivo and in vitro has been elucidated, but the underlying cytotoxicity of TiO(2)-NPs against microRNA remains largely unknown. The purpose of this study was to analyze microRNA profiling induced by TiO(2)-NPs against NCM460 and HCT116 cell lines. Comparative analysis identified 34 and 24 microRNAs were significantly altered in the TiO(2)-NPs treated cells at concentrations of 3 μg/mL and 30 μg/mL, respectively. Functional classification demonstrated that a large proportion of genes involved in metabolism, human disease, and environmental information process were significantly upregulated by TiO(2)-NPs. Bioinformatics analysis suggested that microRNA 378 might be an early indicator of cellular response to exogenous stimuli with apoptotic signals. Furthermore, TiO(2)-NPs significantly altered the expression of microRNA 378b and 378g in HCT116 and NCM460 cell lines at different concentrations from 3 to 6 μg/mL. These concentrations elicit high-sensitivity of stimuli response in colon cancer cells when exposed to the slight doses of TiO(2)-NPs. Our study indicated that microRNAs 378b and 378g may play an important role in TiO(2)-NPs-mediated colonic cytotoxicity, which may provide a valuable insight into the molecular mechanisms of potential risks in colitis and colon cancer.
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spelling pubmed-72814482020-06-19 MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles Li, Wen Jia, Ming Xi Deng, Jing Wang, Jian Hui Zuberi, Zavuga Yang, Sheng Ba, Jie Chen, Zhu Cancers (Basel) Article Titanium dioxide nanoparticles (TiO(2)-NPs) are widely used for biomedical and food applications, the toxicity of TiO(2)-NPs in vivo and in vitro has been elucidated, but the underlying cytotoxicity of TiO(2)-NPs against microRNA remains largely unknown. The purpose of this study was to analyze microRNA profiling induced by TiO(2)-NPs against NCM460 and HCT116 cell lines. Comparative analysis identified 34 and 24 microRNAs were significantly altered in the TiO(2)-NPs treated cells at concentrations of 3 μg/mL and 30 μg/mL, respectively. Functional classification demonstrated that a large proportion of genes involved in metabolism, human disease, and environmental information process were significantly upregulated by TiO(2)-NPs. Bioinformatics analysis suggested that microRNA 378 might be an early indicator of cellular response to exogenous stimuli with apoptotic signals. Furthermore, TiO(2)-NPs significantly altered the expression of microRNA 378b and 378g in HCT116 and NCM460 cell lines at different concentrations from 3 to 6 μg/mL. These concentrations elicit high-sensitivity of stimuli response in colon cancer cells when exposed to the slight doses of TiO(2)-NPs. Our study indicated that microRNAs 378b and 378g may play an important role in TiO(2)-NPs-mediated colonic cytotoxicity, which may provide a valuable insight into the molecular mechanisms of potential risks in colitis and colon cancer. MDPI 2020-05-14 /pmc/articles/PMC7281448/ /pubmed/32423014 http://dx.doi.org/10.3390/cancers12051236 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Wen
Jia, Ming Xi
Deng, Jing
Wang, Jian Hui
Zuberi, Zavuga
Yang, Sheng
Ba, Jie
Chen, Zhu
MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles
title MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles
title_full MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles
title_fullStr MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles
title_full_unstemmed MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles
title_short MicroRNA Response and Toxicity of Potential Pathways in Human Colon Cancer Cells Exposed to Titanium Dioxide Nanoparticles
title_sort microrna response and toxicity of potential pathways in human colon cancer cells exposed to titanium dioxide nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281448/
https://www.ncbi.nlm.nih.gov/pubmed/32423014
http://dx.doi.org/10.3390/cancers12051236
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