Cargando…
Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor
Thrombin generation is a complex and finely regulated pathway that provokes dynamical changes of thrombin concentration in blood when a vascular injury occurs. In order to characterize the initiation phase of such process, when thrombin concentration is in the nM range, a label-free optical aptasens...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281654/ https://www.ncbi.nlm.nih.gov/pubmed/32354154 http://dx.doi.org/10.3390/mi11050464 |
_version_ | 1783543970718023680 |
---|---|
author | Martínez-Pérez, Paula Gómez-Gómez, Maribel Angelova, Todora Griol, Amadeu Hurtado, Juan Bellieres, Laurent García-Rupérez, Jaime |
author_facet | Martínez-Pérez, Paula Gómez-Gómez, Maribel Angelova, Todora Griol, Amadeu Hurtado, Juan Bellieres, Laurent García-Rupérez, Jaime |
author_sort | Martínez-Pérez, Paula |
collection | PubMed |
description | Thrombin generation is a complex and finely regulated pathway that provokes dynamical changes of thrombin concentration in blood when a vascular injury occurs. In order to characterize the initiation phase of such process, when thrombin concentration is in the nM range, a label-free optical aptasensor is proposed here. This aptasensor combines a 1D photonic crystal structure consisting of a silicon corrugated waveguide with thrombin binding aptamers on its surface as bioreceptors. As a result, this aptasensor has been demonstrated to specifically detect thrombin concentrations ranging from 270 pM to 27 nM with an estimated detection limit of 33.5 pM and a response time of ~2 min. Furthermore, it has also been demonstrated that this aptasensor is able to continuously respond to consecutive increasing concentrations of thrombin and to detect binding events as they occur. All these features make this aptasensor a good candidate to continuously study how thrombin concentration progressively increases during the initiation phase of the coagulation cascade. |
format | Online Article Text |
id | pubmed-7281654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72816542020-06-17 Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor Martínez-Pérez, Paula Gómez-Gómez, Maribel Angelova, Todora Griol, Amadeu Hurtado, Juan Bellieres, Laurent García-Rupérez, Jaime Micromachines (Basel) Article Thrombin generation is a complex and finely regulated pathway that provokes dynamical changes of thrombin concentration in blood when a vascular injury occurs. In order to characterize the initiation phase of such process, when thrombin concentration is in the nM range, a label-free optical aptasensor is proposed here. This aptasensor combines a 1D photonic crystal structure consisting of a silicon corrugated waveguide with thrombin binding aptamers on its surface as bioreceptors. As a result, this aptasensor has been demonstrated to specifically detect thrombin concentrations ranging from 270 pM to 27 nM with an estimated detection limit of 33.5 pM and a response time of ~2 min. Furthermore, it has also been demonstrated that this aptasensor is able to continuously respond to consecutive increasing concentrations of thrombin and to detect binding events as they occur. All these features make this aptasensor a good candidate to continuously study how thrombin concentration progressively increases during the initiation phase of the coagulation cascade. MDPI 2020-04-28 /pmc/articles/PMC7281654/ /pubmed/32354154 http://dx.doi.org/10.3390/mi11050464 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Martínez-Pérez, Paula Gómez-Gómez, Maribel Angelova, Todora Griol, Amadeu Hurtado, Juan Bellieres, Laurent García-Rupérez, Jaime Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor |
title | Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor |
title_full | Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor |
title_fullStr | Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor |
title_full_unstemmed | Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor |
title_short | Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor |
title_sort | continuous detection of increasing concentrations of thrombin employing a label-free photonic crystal aptasensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281654/ https://www.ncbi.nlm.nih.gov/pubmed/32354154 http://dx.doi.org/10.3390/mi11050464 |
work_keys_str_mv | AT martinezperezpaula continuousdetectionofincreasingconcentrationsofthrombinemployingalabelfreephotoniccrystalaptasensor AT gomezgomezmaribel continuousdetectionofincreasingconcentrationsofthrombinemployingalabelfreephotoniccrystalaptasensor AT angelovatodora continuousdetectionofincreasingconcentrationsofthrombinemployingalabelfreephotoniccrystalaptasensor AT griolamadeu continuousdetectionofincreasingconcentrationsofthrombinemployingalabelfreephotoniccrystalaptasensor AT hurtadojuan continuousdetectionofincreasingconcentrationsofthrombinemployingalabelfreephotoniccrystalaptasensor AT belliereslaurent continuousdetectionofincreasingconcentrationsofthrombinemployingalabelfreephotoniccrystalaptasensor AT garciaruperezjaime continuousdetectionofincreasingconcentrationsofthrombinemployingalabelfreephotoniccrystalaptasensor |