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Development of a Portable SPR Sensor for Nucleic Acid Detection
Nucleic acid detection is of great significance in clinical diagnosis, environmental monitoring and food safety. Compared with the traditional nucleic acid amplification detection method, surface plasmon resonance (SPR) sensing technology has the advantages of being label-free, having simple operati...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281666/ https://www.ncbi.nlm.nih.gov/pubmed/32455736 http://dx.doi.org/10.3390/mi11050526 |
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author | Huang, Yafeng Zhang, Lulu Zhang, Hao Li, Yichen Liu, Luyao Chen, Yuanyuan Qiu, Xianbo Yu, Duli |
author_facet | Huang, Yafeng Zhang, Lulu Zhang, Hao Li, Yichen Liu, Luyao Chen, Yuanyuan Qiu, Xianbo Yu, Duli |
author_sort | Huang, Yafeng |
collection | PubMed |
description | Nucleic acid detection is of great significance in clinical diagnosis, environmental monitoring and food safety. Compared with the traditional nucleic acid amplification detection method, surface plasmon resonance (SPR) sensing technology has the advantages of being label-free, having simple operation, and providing real-time detection. However, the angle scanning system in many SPR angle modulation detection applications usually requires a high-resolution stepper motor and complex mechanical structure to adjust the angle. In this paper, a portable multi-angle scanning SPR sensor was designed. The sensor only uses one stepping motor to rotate a belt, and the belt pulls the mechanical linkages of incident light and reflected light to move in opposite directions for achieving the SPR angle scanning mode that keeps the incident angle and reflected angle equal. The sensor has an angle scanning accuracy of 0.002°, response sensitivity of 3.72 × 10(−6) RIU (refractive index unit), and an angle scanning range of 30°–74°. The overall size of the system is only 480 mm × 150 mm × 180 mm. The portable SPR sensor was used to detect nucleic acid hybridization on a gold film chip modified with bovine serum albumin (BSA). The result revealed that the sensor had high sensitivity and fast response, and could successfully accomplish the hybridization detection of target DNA solution of 0.01 μmol/mL. |
format | Online Article Text |
id | pubmed-7281666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72816662020-06-15 Development of a Portable SPR Sensor for Nucleic Acid Detection Huang, Yafeng Zhang, Lulu Zhang, Hao Li, Yichen Liu, Luyao Chen, Yuanyuan Qiu, Xianbo Yu, Duli Micromachines (Basel) Article Nucleic acid detection is of great significance in clinical diagnosis, environmental monitoring and food safety. Compared with the traditional nucleic acid amplification detection method, surface plasmon resonance (SPR) sensing technology has the advantages of being label-free, having simple operation, and providing real-time detection. However, the angle scanning system in many SPR angle modulation detection applications usually requires a high-resolution stepper motor and complex mechanical structure to adjust the angle. In this paper, a portable multi-angle scanning SPR sensor was designed. The sensor only uses one stepping motor to rotate a belt, and the belt pulls the mechanical linkages of incident light and reflected light to move in opposite directions for achieving the SPR angle scanning mode that keeps the incident angle and reflected angle equal. The sensor has an angle scanning accuracy of 0.002°, response sensitivity of 3.72 × 10(−6) RIU (refractive index unit), and an angle scanning range of 30°–74°. The overall size of the system is only 480 mm × 150 mm × 180 mm. The portable SPR sensor was used to detect nucleic acid hybridization on a gold film chip modified with bovine serum albumin (BSA). The result revealed that the sensor had high sensitivity and fast response, and could successfully accomplish the hybridization detection of target DNA solution of 0.01 μmol/mL. MDPI 2020-05-21 /pmc/articles/PMC7281666/ /pubmed/32455736 http://dx.doi.org/10.3390/mi11050526 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Yafeng Zhang, Lulu Zhang, Hao Li, Yichen Liu, Luyao Chen, Yuanyuan Qiu, Xianbo Yu, Duli Development of a Portable SPR Sensor for Nucleic Acid Detection |
title | Development of a Portable SPR Sensor for Nucleic Acid Detection |
title_full | Development of a Portable SPR Sensor for Nucleic Acid Detection |
title_fullStr | Development of a Portable SPR Sensor for Nucleic Acid Detection |
title_full_unstemmed | Development of a Portable SPR Sensor for Nucleic Acid Detection |
title_short | Development of a Portable SPR Sensor for Nucleic Acid Detection |
title_sort | development of a portable spr sensor for nucleic acid detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281666/ https://www.ncbi.nlm.nih.gov/pubmed/32455736 http://dx.doi.org/10.3390/mi11050526 |
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