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Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018

Salmonellosis is a major cause of bacterial foodborne infection. Since 2016, an increased number of cases of gastroenteritis caused by Salmonella enterica serovar Enteritidis linked to eggs produced in Poland has been reported in Europe. In Italy, S. Enteritidis is one of the three most commonly rep...

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Autores principales: Di Marcantonio, Lisa, Janowicz, Anna, Zilli, Katiuscia, Romantini, Romina, Bilei, Stefano, Paganico, Daniela, Persiani, Tiziana, Di Donato, Guido, Di Giannatale, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281747/
https://www.ncbi.nlm.nih.gov/pubmed/32380662
http://dx.doi.org/10.3390/pathogens9050349
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author Di Marcantonio, Lisa
Janowicz, Anna
Zilli, Katiuscia
Romantini, Romina
Bilei, Stefano
Paganico, Daniela
Persiani, Tiziana
Di Donato, Guido
Di Giannatale, Elisabetta
author_facet Di Marcantonio, Lisa
Janowicz, Anna
Zilli, Katiuscia
Romantini, Romina
Bilei, Stefano
Paganico, Daniela
Persiani, Tiziana
Di Donato, Guido
Di Giannatale, Elisabetta
author_sort Di Marcantonio, Lisa
collection PubMed
description Salmonellosis is a major cause of bacterial foodborne infection. Since 2016, an increased number of cases of gastroenteritis caused by Salmonella enterica serovar Enteritidis linked to eggs produced in Poland has been reported in Europe. In Italy, S. Enteritidis is one of the three most commonly reported serotypes, associated mainly with the consumption of contaminated eggs and derived products. In our work, we analysed 61 strains of S. Enteritidis obtained from humans and farms in the Abruzzi region, Italy, in 2018. We used Multiple-Loci Variable-Number Tandem Repeat (VNTR) analysis (MLVA)-based typing and Whole-Genome Sequencing (WGS) tools to identify closely related strains and perform cluster analysis. We found two clusters of genetically similar strains. The first one was present in the local farms and isolated from human cases and had single-linkage distance of no more than two core genes and less than five Single-Nucleotide Polymorphisms (SNPs). The second cluster contained strains isolated from humans and from a dessert (tiramisù) sample that shared identical core genome and were assigned the same SNP address. Cluster 2 isolates were found to be genetically similar to an S. Enteritidis strain from a multi-country outbreak linked to Polish eggs.
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spelling pubmed-72817472020-06-15 Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018 Di Marcantonio, Lisa Janowicz, Anna Zilli, Katiuscia Romantini, Romina Bilei, Stefano Paganico, Daniela Persiani, Tiziana Di Donato, Guido Di Giannatale, Elisabetta Pathogens Article Salmonellosis is a major cause of bacterial foodborne infection. Since 2016, an increased number of cases of gastroenteritis caused by Salmonella enterica serovar Enteritidis linked to eggs produced in Poland has been reported in Europe. In Italy, S. Enteritidis is one of the three most commonly reported serotypes, associated mainly with the consumption of contaminated eggs and derived products. In our work, we analysed 61 strains of S. Enteritidis obtained from humans and farms in the Abruzzi region, Italy, in 2018. We used Multiple-Loci Variable-Number Tandem Repeat (VNTR) analysis (MLVA)-based typing and Whole-Genome Sequencing (WGS) tools to identify closely related strains and perform cluster analysis. We found two clusters of genetically similar strains. The first one was present in the local farms and isolated from human cases and had single-linkage distance of no more than two core genes and less than five Single-Nucleotide Polymorphisms (SNPs). The second cluster contained strains isolated from humans and from a dessert (tiramisù) sample that shared identical core genome and were assigned the same SNP address. Cluster 2 isolates were found to be genetically similar to an S. Enteritidis strain from a multi-country outbreak linked to Polish eggs. MDPI 2020-05-05 /pmc/articles/PMC7281747/ /pubmed/32380662 http://dx.doi.org/10.3390/pathogens9050349 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Marcantonio, Lisa
Janowicz, Anna
Zilli, Katiuscia
Romantini, Romina
Bilei, Stefano
Paganico, Daniela
Persiani, Tiziana
Di Donato, Guido
Di Giannatale, Elisabetta
Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018
title Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018
title_full Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018
title_fullStr Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018
title_full_unstemmed Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018
title_short Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018
title_sort genomic comparison of salmonella enteritidis strains isolated from laying hens and humans in the abruzzi region during 2018
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281747/
https://www.ncbi.nlm.nih.gov/pubmed/32380662
http://dx.doi.org/10.3390/pathogens9050349
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