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Deciphering the Proteome Dynamics during Development of Neurons Derived from Induced Pluripotent Stem Cells
[Image: see text] Neuronal development is a complex multistep process that shapes neurons by progressing though several typical stages, including axon outgrowth, dendrite formation, and synaptogenesis. Knowledge of the mechanisms of neuronal development is mostly derived from the study of animal mod...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281779/ https://www.ncbi.nlm.nih.gov/pubmed/32357013 http://dx.doi.org/10.1021/acs.jproteome.0c00070 |
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author | Varderidou-Minasian, Suzy Verheijen, Bert M. Schätzle, Philipp Hoogenraad, Casper. C. Pasterkamp, R. Jeroen Altelaar, Maarten |
author_facet | Varderidou-Minasian, Suzy Verheijen, Bert M. Schätzle, Philipp Hoogenraad, Casper. C. Pasterkamp, R. Jeroen Altelaar, Maarten |
author_sort | Varderidou-Minasian, Suzy |
collection | PubMed |
description | [Image: see text] Neuronal development is a complex multistep process that shapes neurons by progressing though several typical stages, including axon outgrowth, dendrite formation, and synaptogenesis. Knowledge of the mechanisms of neuronal development is mostly derived from the study of animal models. Advances in stem cell technology now enable us to generate neurons from human induced pluripotent stem cells (iPSCs). Here we provide a mass spectrometry-based quantitative proteomic signature of human iPSC-derived neurons, i.e., iPSC-derived induced glutamatergic neurons and iPSC-derived motor neurons, throughout neuronal differentiation. Tandem mass tag 10-plex labeling was carried out to perform proteomic profiling of cells at different time points. Our analysis reveals significant expression changes (FDR < 0.001) of several key proteins during the differentiation process, e.g., proteins involved in the Wnt and Notch signaling pathways. Overall, our data provide a rich resource of information on protein expression during human iPSC neuron differentiation. |
format | Online Article Text |
id | pubmed-7281779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-72817792020-06-15 Deciphering the Proteome Dynamics during Development of Neurons Derived from Induced Pluripotent Stem Cells Varderidou-Minasian, Suzy Verheijen, Bert M. Schätzle, Philipp Hoogenraad, Casper. C. Pasterkamp, R. Jeroen Altelaar, Maarten J Proteome Res [Image: see text] Neuronal development is a complex multistep process that shapes neurons by progressing though several typical stages, including axon outgrowth, dendrite formation, and synaptogenesis. Knowledge of the mechanisms of neuronal development is mostly derived from the study of animal models. Advances in stem cell technology now enable us to generate neurons from human induced pluripotent stem cells (iPSCs). Here we provide a mass spectrometry-based quantitative proteomic signature of human iPSC-derived neurons, i.e., iPSC-derived induced glutamatergic neurons and iPSC-derived motor neurons, throughout neuronal differentiation. Tandem mass tag 10-plex labeling was carried out to perform proteomic profiling of cells at different time points. Our analysis reveals significant expression changes (FDR < 0.001) of several key proteins during the differentiation process, e.g., proteins involved in the Wnt and Notch signaling pathways. Overall, our data provide a rich resource of information on protein expression during human iPSC neuron differentiation. American Chemical Society 2020-05-01 2020-06-05 /pmc/articles/PMC7281779/ /pubmed/32357013 http://dx.doi.org/10.1021/acs.jproteome.0c00070 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Varderidou-Minasian, Suzy Verheijen, Bert M. Schätzle, Philipp Hoogenraad, Casper. C. Pasterkamp, R. Jeroen Altelaar, Maarten Deciphering the Proteome Dynamics during Development of Neurons Derived from Induced Pluripotent Stem Cells |
title | Deciphering the
Proteome Dynamics during Development
of Neurons Derived from Induced Pluripotent Stem Cells |
title_full | Deciphering the
Proteome Dynamics during Development
of Neurons Derived from Induced Pluripotent Stem Cells |
title_fullStr | Deciphering the
Proteome Dynamics during Development
of Neurons Derived from Induced Pluripotent Stem Cells |
title_full_unstemmed | Deciphering the
Proteome Dynamics during Development
of Neurons Derived from Induced Pluripotent Stem Cells |
title_short | Deciphering the
Proteome Dynamics during Development
of Neurons Derived from Induced Pluripotent Stem Cells |
title_sort | deciphering the
proteome dynamics during development
of neurons derived from induced pluripotent stem cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281779/ https://www.ncbi.nlm.nih.gov/pubmed/32357013 http://dx.doi.org/10.1021/acs.jproteome.0c00070 |
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