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Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial

(1) Background: Alterations in the structural composition of the human gut microbiota have been identified in various disease entities along with exciting mechanistic clues by reductionist gnotobiotic modeling. Improving health by beneficially modulating an altered microbiota is a promising treatmen...

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Autores principales: Reider, Simon J., Moosmang, Simon, Tragust, Judith, Trgovec-Greif, Lovro, Tragust, Simon, Perschy, Lorenz, Przysiecki, Nicole, Sturm, Sonja, Tilg, Herbert, Stuppner, Hermann, Rattei, Thomas, Moschen, Alexander R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281958/
https://www.ncbi.nlm.nih.gov/pubmed/32354152
http://dx.doi.org/10.3390/nu12051257
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author Reider, Simon J.
Moosmang, Simon
Tragust, Judith
Trgovec-Greif, Lovro
Tragust, Simon
Perschy, Lorenz
Przysiecki, Nicole
Sturm, Sonja
Tilg, Herbert
Stuppner, Hermann
Rattei, Thomas
Moschen, Alexander R.
author_facet Reider, Simon J.
Moosmang, Simon
Tragust, Judith
Trgovec-Greif, Lovro
Tragust, Simon
Perschy, Lorenz
Przysiecki, Nicole
Sturm, Sonja
Tilg, Herbert
Stuppner, Hermann
Rattei, Thomas
Moschen, Alexander R.
author_sort Reider, Simon J.
collection PubMed
description (1) Background: Alterations in the structural composition of the human gut microbiota have been identified in various disease entities along with exciting mechanistic clues by reductionist gnotobiotic modeling. Improving health by beneficially modulating an altered microbiota is a promising treatment approach. Prebiotics, substrates selectively used by host microorganisms conferring a health benefit, are broadly used for dietary and clinical interventions. Herein, we sought to investigate the microbiota-modelling effects of the soluble fiber, partially hydrolyzed guar gum (PHGG). (2) Methods: We performed a 9 week clinical trial in 20 healthy volunteers that included three weeks of a lead-in period, followed by three weeks of an intervention phase, wherein study subjects received 5 g PHGG up to three times per day, and concluding with a three-week washout period. A stool diary was kept on a daily basis, and clinical data along with serum/plasma and stool samples were collected on a weekly basis. PHGG-induced alterations of the gut microbiota were studied by 16S metagenomics of the V1–V3 and V3–V4 regions. To gain functional insight, we further studied stool metabolites using nuclear magnetic resonance (NMR) spectroscopy. (3) Results: In healthy subjects, PHGG had significant effects on stool frequency and consistency. These effects were paralleled by changes in α- (species evenness) and β-diversity (Bray–Curtis distances), along with increasing abundances of metabolites including butyrate, acetate and various amino acids. On a taxonomic level, PHGG intake was associated with a bloom in Ruminococcus, Fusicatenibacter, Faecalibacterium and Bacteroides and a reduction in Roseburia, Lachnospiracea and Blautia. The majority of effects disappeared after stopping the prebiotic and most effects tended to be more pronounced in male participants. (4) Conclusions: Herein, we describe novel aspects of the prebiotic PHGG on compositional and functional properties of the healthy human microbiota.
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spelling pubmed-72819582020-06-15 Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial Reider, Simon J. Moosmang, Simon Tragust, Judith Trgovec-Greif, Lovro Tragust, Simon Perschy, Lorenz Przysiecki, Nicole Sturm, Sonja Tilg, Herbert Stuppner, Hermann Rattei, Thomas Moschen, Alexander R. Nutrients Article (1) Background: Alterations in the structural composition of the human gut microbiota have been identified in various disease entities along with exciting mechanistic clues by reductionist gnotobiotic modeling. Improving health by beneficially modulating an altered microbiota is a promising treatment approach. Prebiotics, substrates selectively used by host microorganisms conferring a health benefit, are broadly used for dietary and clinical interventions. Herein, we sought to investigate the microbiota-modelling effects of the soluble fiber, partially hydrolyzed guar gum (PHGG). (2) Methods: We performed a 9 week clinical trial in 20 healthy volunteers that included three weeks of a lead-in period, followed by three weeks of an intervention phase, wherein study subjects received 5 g PHGG up to three times per day, and concluding with a three-week washout period. A stool diary was kept on a daily basis, and clinical data along with serum/plasma and stool samples were collected on a weekly basis. PHGG-induced alterations of the gut microbiota were studied by 16S metagenomics of the V1–V3 and V3–V4 regions. To gain functional insight, we further studied stool metabolites using nuclear magnetic resonance (NMR) spectroscopy. (3) Results: In healthy subjects, PHGG had significant effects on stool frequency and consistency. These effects were paralleled by changes in α- (species evenness) and β-diversity (Bray–Curtis distances), along with increasing abundances of metabolites including butyrate, acetate and various amino acids. On a taxonomic level, PHGG intake was associated with a bloom in Ruminococcus, Fusicatenibacter, Faecalibacterium and Bacteroides and a reduction in Roseburia, Lachnospiracea and Blautia. The majority of effects disappeared after stopping the prebiotic and most effects tended to be more pronounced in male participants. (4) Conclusions: Herein, we describe novel aspects of the prebiotic PHGG on compositional and functional properties of the healthy human microbiota. MDPI 2020-04-28 /pmc/articles/PMC7281958/ /pubmed/32354152 http://dx.doi.org/10.3390/nu12051257 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reider, Simon J.
Moosmang, Simon
Tragust, Judith
Trgovec-Greif, Lovro
Tragust, Simon
Perschy, Lorenz
Przysiecki, Nicole
Sturm, Sonja
Tilg, Herbert
Stuppner, Hermann
Rattei, Thomas
Moschen, Alexander R.
Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial
title Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial
title_full Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial
title_fullStr Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial
title_full_unstemmed Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial
title_short Prebiotic Effects of Partially Hydrolyzed Guar Gum on the Composition and Function of the Human Microbiota—Results from the PAGODA Trial
title_sort prebiotic effects of partially hydrolyzed guar gum on the composition and function of the human microbiota—results from the pagoda trial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281958/
https://www.ncbi.nlm.nih.gov/pubmed/32354152
http://dx.doi.org/10.3390/nu12051257
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