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Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy

Lower back pain (LBP) is one of the most common musculoskeletal complaints worldwide. Intervertebral disc degeneration (IDD) is considered to be a significant contributor to LBP; however, the mechanisms underlying IDD remain to be fully elucidated. One of the major features of IDD is the decreased c...

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Autores principales: Wang, Xuehui, Meng, Jianfang, Zhang, Tongxing, Weijia Lv, William, Liang, Zhao, Shi, Qian, Li, Zhaoyang, Zhang, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281961/
https://www.ncbi.nlm.nih.gov/pubmed/32537009
http://dx.doi.org/10.3892/etm.2020.8729
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author Wang, Xuehui
Meng, Jianfang
Zhang, Tongxing
Weijia Lv, William
Liang, Zhao
Shi, Qian
Li, Zhaoyang
Zhang, Tao
author_facet Wang, Xuehui
Meng, Jianfang
Zhang, Tongxing
Weijia Lv, William
Liang, Zhao
Shi, Qian
Li, Zhaoyang
Zhang, Tao
author_sort Wang, Xuehui
collection PubMed
description Lower back pain (LBP) is one of the most common musculoskeletal complaints worldwide. Intervertebral disc degeneration (IDD) is considered to be a significant contributor to LBP; however, the mechanisms underlying IDD remain to be fully elucidated. One of the major features of IDD is the decreased content of type II collagen and proteoglycans in the nucleus pulposus (NP). The present study aimed to investigate the biochemical mechanisms of IDD at the microscopic level using Raman spectroscopy. Raman spectroscopy, based on inelastic scattering of light, is an emerging optical technique that may measure the chemical composition of complex biological samples, including biofluids, cells and tissues. In the present study, 30 NP tissue samples from 30 patients who were diagnosed with lumbar disc herniation and received spinal fusion surgery to relieve LBP were obtained and analyzed. Routine pre-operative 3.0T, T2-weighed MRI was used to classify the cases according to Pfirrmann grades and the T2 signal intensity value of the NP was measured. Subsequently, all NP samples were scanned and analyzed using a Laser MicroRaman Spectrometer at room temperature. The Raman spectral results demonstrated that the relative content of proteoglycans, expressed as the relative intensity ratio of two peaks (I(1064)/I(1004)), was significantly inversely correlated with the Pfirrmann grade (ρ=-0.6462; P<0.0001), whereas the content of collagen (amide I) was significantly positively correlated with the Pfirrmann grade (ρ=0.5141; P<0.01). In conclusion, the higher relative intensity of the ratio of two peaks (I(1670)/I(1640); Amide I) represented a higher fractional content of disordered collagen, which suggested that the defective collagen structure may lead to NP abnormalities.
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spelling pubmed-72819612020-06-11 Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy Wang, Xuehui Meng, Jianfang Zhang, Tongxing Weijia Lv, William Liang, Zhao Shi, Qian Li, Zhaoyang Zhang, Tao Exp Ther Med Articles Lower back pain (LBP) is one of the most common musculoskeletal complaints worldwide. Intervertebral disc degeneration (IDD) is considered to be a significant contributor to LBP; however, the mechanisms underlying IDD remain to be fully elucidated. One of the major features of IDD is the decreased content of type II collagen and proteoglycans in the nucleus pulposus (NP). The present study aimed to investigate the biochemical mechanisms of IDD at the microscopic level using Raman spectroscopy. Raman spectroscopy, based on inelastic scattering of light, is an emerging optical technique that may measure the chemical composition of complex biological samples, including biofluids, cells and tissues. In the present study, 30 NP tissue samples from 30 patients who were diagnosed with lumbar disc herniation and received spinal fusion surgery to relieve LBP were obtained and analyzed. Routine pre-operative 3.0T, T2-weighed MRI was used to classify the cases according to Pfirrmann grades and the T2 signal intensity value of the NP was measured. Subsequently, all NP samples were scanned and analyzed using a Laser MicroRaman Spectrometer at room temperature. The Raman spectral results demonstrated that the relative content of proteoglycans, expressed as the relative intensity ratio of two peaks (I(1064)/I(1004)), was significantly inversely correlated with the Pfirrmann grade (ρ=-0.6462; P<0.0001), whereas the content of collagen (amide I) was significantly positively correlated with the Pfirrmann grade (ρ=0.5141; P<0.01). In conclusion, the higher relative intensity of the ratio of two peaks (I(1670)/I(1640); Amide I) represented a higher fractional content of disordered collagen, which suggested that the defective collagen structure may lead to NP abnormalities. D.A. Spandidos 2020-07 2020-05-07 /pmc/articles/PMC7281961/ /pubmed/32537009 http://dx.doi.org/10.3892/etm.2020.8729 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Xuehui
Meng, Jianfang
Zhang, Tongxing
Weijia Lv, William
Liang, Zhao
Shi, Qian
Li, Zhaoyang
Zhang, Tao
Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy
title Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy
title_full Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy
title_fullStr Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy
title_full_unstemmed Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy
title_short Identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using Raman spectroscopy
title_sort identifying compositional and structural changes in the nucleus pulposus from patients with lumbar disc herniation using raman spectroscopy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281961/
https://www.ncbi.nlm.nih.gov/pubmed/32537009
http://dx.doi.org/10.3892/etm.2020.8729
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