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Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions

BACKGROUND: Identification of the allelopathy-interrelated metabolites from the allelopathic rice rhizosphere is crucial to understand the allelopathic mechanism of rice, which in turn can promote its applications to farming. In this study, the metabolites from the rhizosphere soil of five different...

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Autores principales: Li, Yingzhe, Xu, Lining, Letuma, Puleng, Lin, Wenxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282037/
https://www.ncbi.nlm.nih.gov/pubmed/32517652
http://dx.doi.org/10.1186/s12870-020-02465-6
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author Li, Yingzhe
Xu, Lining
Letuma, Puleng
Lin, Wenxiong
author_facet Li, Yingzhe
Xu, Lining
Letuma, Puleng
Lin, Wenxiong
author_sort Li, Yingzhe
collection PubMed
description BACKGROUND: Identification of the allelopathy-interrelated metabolites from the allelopathic rice rhizosphere is crucial to understand the allelopathic mechanism of rice, which in turn can promote its applications to farming. In this study, the metabolites from the rhizosphere soil of five different rice lines, including allelopathic rice accession PI312777 (PI) and non-allelopathic rice accession Lemont (Le) as well as their genetic derivatives (e.g., phenylalanine ammonia-lyase (PAL) gene overexpression transgenic lines of PI and Le, namely, PO and LO respectively, and PAL RNA interference line of PI, namely, PR) were identified and comparatively analyzed to explore the positive compounds that are involved in the process of rice allelopathy. RESULTS: The results showed that 21 non-polar compounds and 21 polar compounds differed in content in the rhizosphere soil of PI and Le, which include several volatile fatty acids and long-chain fatty acids. The relative contents of fatty acids also differed between PAL overexpressing or RNA interference (RNAi)-silenced line and their wild-type respectively. Acetic acid content also differed among groups, i.e., it is higher in the high allelopathic potential rice. Further analysis showed that different metabolites from the ADS8 resin-extracted phase were more abundant than that those from the ADS21 resin-extracted phase, suggesting that the allelochemicals in root exudates of allelopathic rice are mainly non-polar substances. KEGG annotation of these differential metabolites revealed that these compounds were related to nutrient metabolism, secondary metabolite synthesis, signaling substance synthesis, and toxin degradation. CONCLUSIONS: Rice allelochemicals deposited in the ADS8 resin-extracted phase were more abundant than those in the ADS21 resin-extracted phase. Allelochemicals in root exudates of allelopathic rice are mainly non-polar substances, and long-chain fatty acids are considered as allelopathy interrelated metabolites.
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spelling pubmed-72820372020-06-10 Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions Li, Yingzhe Xu, Lining Letuma, Puleng Lin, Wenxiong BMC Plant Biol Research Article BACKGROUND: Identification of the allelopathy-interrelated metabolites from the allelopathic rice rhizosphere is crucial to understand the allelopathic mechanism of rice, which in turn can promote its applications to farming. In this study, the metabolites from the rhizosphere soil of five different rice lines, including allelopathic rice accession PI312777 (PI) and non-allelopathic rice accession Lemont (Le) as well as their genetic derivatives (e.g., phenylalanine ammonia-lyase (PAL) gene overexpression transgenic lines of PI and Le, namely, PO and LO respectively, and PAL RNA interference line of PI, namely, PR) were identified and comparatively analyzed to explore the positive compounds that are involved in the process of rice allelopathy. RESULTS: The results showed that 21 non-polar compounds and 21 polar compounds differed in content in the rhizosphere soil of PI and Le, which include several volatile fatty acids and long-chain fatty acids. The relative contents of fatty acids also differed between PAL overexpressing or RNA interference (RNAi)-silenced line and their wild-type respectively. Acetic acid content also differed among groups, i.e., it is higher in the high allelopathic potential rice. Further analysis showed that different metabolites from the ADS8 resin-extracted phase were more abundant than that those from the ADS21 resin-extracted phase, suggesting that the allelochemicals in root exudates of allelopathic rice are mainly non-polar substances. KEGG annotation of these differential metabolites revealed that these compounds were related to nutrient metabolism, secondary metabolite synthesis, signaling substance synthesis, and toxin degradation. CONCLUSIONS: Rice allelochemicals deposited in the ADS8 resin-extracted phase were more abundant than those in the ADS21 resin-extracted phase. Allelochemicals in root exudates of allelopathic rice are mainly non-polar substances, and long-chain fatty acids are considered as allelopathy interrelated metabolites. BioMed Central 2020-06-09 /pmc/articles/PMC7282037/ /pubmed/32517652 http://dx.doi.org/10.1186/s12870-020-02465-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Li, Yingzhe
Xu, Lining
Letuma, Puleng
Lin, Wenxiong
Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions
title Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions
title_full Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions
title_fullStr Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions
title_full_unstemmed Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions
title_short Metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions
title_sort metabolite profiling of rhizosphere soil of different allelopathic potential rice accessions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282037/
https://www.ncbi.nlm.nih.gov/pubmed/32517652
http://dx.doi.org/10.1186/s12870-020-02465-6
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