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Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages
BACKGROUND: Tuberculosis is an important health problem worldwide. The only available vaccine is M. bovis/BCG, an attenuated mycobacterium that activates the innate and the acquired immune system after being phagocytosed by macrophages and dendritic cells. Vaccination fails to prevent adult pulmonar...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282091/ https://www.ncbi.nlm.nih.gov/pubmed/32517651 http://dx.doi.org/10.1186/s12865-020-00364-x |
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author | Espinosa-Cueto, Patricia Magallanes-Puebla, Alejandro Mancilla, Raul |
author_facet | Espinosa-Cueto, Patricia Magallanes-Puebla, Alejandro Mancilla, Raul |
author_sort | Espinosa-Cueto, Patricia |
collection | PubMed |
description | BACKGROUND: Tuberculosis is an important health problem worldwide. The only available vaccine is M. bovis/BCG, an attenuated mycobacterium that activates the innate and the acquired immune system after being phagocytosed by macrophages and dendritic cells. Vaccination fails to prevent adult pulmonary tuberculosis although it may have a protective effect in childhood infection. Understanding how BCG interacts with macrophages and other immunocompetent cells is crucial to develop new vaccines. RESULTS: In this study we showed that macrophages phagocytose M. bovis/BCG bacilli with higher efficiency when they are cultured without phosphate. We isolated mycobacterial membranes to search for mycobacterial molecules that could be involved in these processes; by immunoblot, it was found that the plasma membranes of phosphate-deprived bacilli express the adhesins PstS-1, LpqH, LprG, and the APA antigen. These proteins are not detected in membranes of bacilli grown with usual amounts of phosphate. CONCLUSIONS: The interest of our observations is to show that under the metabolic stress implied in phosphate deprivation, mycobacteria respond upregulating adhesins that could improve their capacity to infect macrophages. These observations are relevant to understand how M. bovis/BCG induces protective immunity. |
format | Online Article Text |
id | pubmed-7282091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72820912020-06-10 Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages Espinosa-Cueto, Patricia Magallanes-Puebla, Alejandro Mancilla, Raul BMC Immunol Research Article BACKGROUND: Tuberculosis is an important health problem worldwide. The only available vaccine is M. bovis/BCG, an attenuated mycobacterium that activates the innate and the acquired immune system after being phagocytosed by macrophages and dendritic cells. Vaccination fails to prevent adult pulmonary tuberculosis although it may have a protective effect in childhood infection. Understanding how BCG interacts with macrophages and other immunocompetent cells is crucial to develop new vaccines. RESULTS: In this study we showed that macrophages phagocytose M. bovis/BCG bacilli with higher efficiency when they are cultured without phosphate. We isolated mycobacterial membranes to search for mycobacterial molecules that could be involved in these processes; by immunoblot, it was found that the plasma membranes of phosphate-deprived bacilli express the adhesins PstS-1, LpqH, LprG, and the APA antigen. These proteins are not detected in membranes of bacilli grown with usual amounts of phosphate. CONCLUSIONS: The interest of our observations is to show that under the metabolic stress implied in phosphate deprivation, mycobacteria respond upregulating adhesins that could improve their capacity to infect macrophages. These observations are relevant to understand how M. bovis/BCG induces protective immunity. BioMed Central 2020-06-09 /pmc/articles/PMC7282091/ /pubmed/32517651 http://dx.doi.org/10.1186/s12865-020-00364-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Espinosa-Cueto, Patricia Magallanes-Puebla, Alejandro Mancilla, Raul Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages |
title | Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages |
title_full | Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages |
title_fullStr | Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages |
title_full_unstemmed | Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages |
title_short | Phosphate starvation enhances phagocytosis of Mycobacterium bovis/BCG by macrophages |
title_sort | phosphate starvation enhances phagocytosis of mycobacterium bovis/bcg by macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282091/ https://www.ncbi.nlm.nih.gov/pubmed/32517651 http://dx.doi.org/10.1186/s12865-020-00364-x |
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