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Thrombin generation in different commercial sodium citrate blood tubes
BACKGROUND: This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. METHODS: Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Medical Biochemists of Serbia, Belgrade
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282229/ https://www.ncbi.nlm.nih.gov/pubmed/32549773 http://dx.doi.org/10.2478/jomb-2019-0016 |
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author | Luca, Salvagno Gian Demonte, Davide Gelati, Matteo Poli, Giovanni Favaloro, Emmanuel J. Lippi, Giuseppe |
author_facet | Luca, Salvagno Gian Demonte, Davide Gelati, Matteo Poli, Giovanni Favaloro, Emmanuel J. Lippi, Giuseppe |
author_sort | Luca, Salvagno Gian |
collection | PubMed |
description | BACKGROUND: This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. METHODS: Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin generation was measured in plasma with the fully-automated ST Genesia analyzer using the weakest trigger (STG-BleedScreen). RESULTS: Different values of lag time (LT), time to reach thrombin peak (TP), thrombin peak height (PH) and endogenous thrombin potential (ETP) were commonly found in different tubes. Thrombin generation was the lowest in 4.3 mL Sarstedt tubes and the highest in 1.0 mL Greiner tubes. Other tubes displayed intermediate values. In multiple comparisons, LT was significantly different in 6/15 cases (40%), whilst PH, TP and ETP were significantly different in 14/15 (93%), 13/15 (87%) and 13/15 (87%) cases. The mean percent bias of LT, PH, TP and ETP ranged between -6% and +1%, -27% and +116%, -22% and +8%, and between -18% and +65%. The intra-assay imprecision of LT, PH, TP and ETP was exceeded in 0/15 (0%), 13/15 (87%), 6/15 (40%) and 13/15 (87%) comparisons. The correlation of LT, PH, TP and ETP values in different tubes ranged between 0.718-0.971, 0.570-0.966, 0.725-0.977 and 0.101-0.904. CONCLUSIONS: Blood collection for thrombin generation assays requires local standardization using identical tubes for brand and draw volume, and reference ranges calculated according to type of tubes. |
format | Online Article Text |
id | pubmed-7282229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Society of Medical Biochemists of Serbia, Belgrade |
record_format | MEDLINE/PubMed |
spelling | pubmed-72822292020-06-16 Thrombin generation in different commercial sodium citrate blood tubes Luca, Salvagno Gian Demonte, Davide Gelati, Matteo Poli, Giovanni Favaloro, Emmanuel J. Lippi, Giuseppe J Med Biochem Original Paper BACKGROUND: This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. METHODS: Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin generation was measured in plasma with the fully-automated ST Genesia analyzer using the weakest trigger (STG-BleedScreen). RESULTS: Different values of lag time (LT), time to reach thrombin peak (TP), thrombin peak height (PH) and endogenous thrombin potential (ETP) were commonly found in different tubes. Thrombin generation was the lowest in 4.3 mL Sarstedt tubes and the highest in 1.0 mL Greiner tubes. Other tubes displayed intermediate values. In multiple comparisons, LT was significantly different in 6/15 cases (40%), whilst PH, TP and ETP were significantly different in 14/15 (93%), 13/15 (87%) and 13/15 (87%) cases. The mean percent bias of LT, PH, TP and ETP ranged between -6% and +1%, -27% and +116%, -22% and +8%, and between -18% and +65%. The intra-assay imprecision of LT, PH, TP and ETP was exceeded in 0/15 (0%), 13/15 (87%), 6/15 (40%) and 13/15 (87%) comparisons. The correlation of LT, PH, TP and ETP values in different tubes ranged between 0.718-0.971, 0.570-0.966, 0.725-0.977 and 0.101-0.904. CONCLUSIONS: Blood collection for thrombin generation assays requires local standardization using identical tubes for brand and draw volume, and reference ranges calculated according to type of tubes. Society of Medical Biochemists of Serbia, Belgrade 2020-01-10 2020-01-10 /pmc/articles/PMC7282229/ /pubmed/32549773 http://dx.doi.org/10.2478/jomb-2019-0016 Text en 2020 Salvagno Gian Luca, Davide Demonte, Matteo Gelati, Giovanni Poli, Emmanuel J. Favaloro, Giuseppe Lippi, published by CEON/CEES https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution 4.0 License. |
spellingShingle | Original Paper Luca, Salvagno Gian Demonte, Davide Gelati, Matteo Poli, Giovanni Favaloro, Emmanuel J. Lippi, Giuseppe Thrombin generation in different commercial sodium citrate blood tubes |
title | Thrombin generation in different commercial sodium citrate blood tubes |
title_full | Thrombin generation in different commercial sodium citrate blood tubes |
title_fullStr | Thrombin generation in different commercial sodium citrate blood tubes |
title_full_unstemmed | Thrombin generation in different commercial sodium citrate blood tubes |
title_short | Thrombin generation in different commercial sodium citrate blood tubes |
title_sort | thrombin generation in different commercial sodium citrate blood tubes |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282229/ https://www.ncbi.nlm.nih.gov/pubmed/32549773 http://dx.doi.org/10.2478/jomb-2019-0016 |
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