Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model

PURPOSE: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. METHODS: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adapti...

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Detalles Bibliográficos
Autores principales: Meirelles, Rogério José de Azevedo, Lizarte, Fermino Sanches, Cirino, Mucio Luiz de Assis, Novais, Paulo Cezar, Gula, Isabella Stracieri, da Silva, Jairo Pinheiro, Tazzima, Maria de Fátima Galli Sorita, Fazan, Valéria Paula Sassoli, Durand, Marina Toledo, Tirapelli, Daniela Pretti da Cunha, de Carvalho, Camila Albuquerque Melo, Schimming, Bruno César, Molina, Carlos Augusto Fernandes, Tucci, Silvio, Tirapelli, Luis Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282493/
https://www.ncbi.nlm.nih.gov/pubmed/32520081
http://dx.doi.org/10.1590/s0102-865020200030000005
Descripción
Sumario:PURPOSE: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. METHODS: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue. RESULTS: Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups. CONCLUSION: Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression.

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