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Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model
PURPOSE: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. METHODS: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adapti...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282493/ https://www.ncbi.nlm.nih.gov/pubmed/32520081 http://dx.doi.org/10.1590/s0102-865020200030000005 |
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| author | Meirelles, Rogério José de Azevedo Lizarte, Fermino Sanches Cirino, Mucio Luiz de Assis Novais, Paulo Cezar Gula, Isabella Stracieri da Silva, Jairo Pinheiro Tazzima, Maria de Fátima Galli Sorita Fazan, Valéria Paula Sassoli Durand, Marina Toledo Tirapelli, Daniela Pretti da Cunha de Carvalho, Camila Albuquerque Melo Schimming, Bruno César Molina, Carlos Augusto Fernandes Tucci, Silvio Tirapelli, Luis Fernando |
| author_facet | Meirelles, Rogério José de Azevedo Lizarte, Fermino Sanches Cirino, Mucio Luiz de Assis Novais, Paulo Cezar Gula, Isabella Stracieri da Silva, Jairo Pinheiro Tazzima, Maria de Fátima Galli Sorita Fazan, Valéria Paula Sassoli Durand, Marina Toledo Tirapelli, Daniela Pretti da Cunha de Carvalho, Camila Albuquerque Melo Schimming, Bruno César Molina, Carlos Augusto Fernandes Tucci, Silvio Tirapelli, Luis Fernando |
| author_sort | Meirelles, Rogério José de Azevedo |
| collection | PubMed |
| description | PURPOSE: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. METHODS: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue. RESULTS: Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups. CONCLUSION: Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression. |
| format | Online Article Text |
| id | pubmed-7282493 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-72824932020-06-17 Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model Meirelles, Rogério José de Azevedo Lizarte, Fermino Sanches Cirino, Mucio Luiz de Assis Novais, Paulo Cezar Gula, Isabella Stracieri da Silva, Jairo Pinheiro Tazzima, Maria de Fátima Galli Sorita Fazan, Valéria Paula Sassoli Durand, Marina Toledo Tirapelli, Daniela Pretti da Cunha de Carvalho, Camila Albuquerque Melo Schimming, Bruno César Molina, Carlos Augusto Fernandes Tucci, Silvio Tirapelli, Luis Fernando Acta Cir Bras Original Article PURPOSE: To evaluate the effect of chronic alcoholism on morphometry and apoptosis mechanism and correlate with miRNA-21 expression in the corpus cavernosum of rats. METHODS: Twenty-four rats were divided into two experimental groups: Control (C) and Alcoholic group (A). After two weeks of an adaptive phase, rats from group A received only ethanol solution (20%) during 7 weeks. The morphometric and caspase-3 immunohistochemistry analysis were performed in the corpus cavernosum. The miRNA-21 expression was analyzed in blood and cavernous tissue. RESULTS: Chronic ethanol consumption decreased cavernosal smooth muscle area of alcoholic rats. The protein expression of caspase 3 in the corpus cavernosum was higher in A compared to the C group. There was no difference in the expression of miRNA-21 in serum and cavernous tissue between the groups. CONCLUSION: Chronic ethanol consumption reduced smooth muscle area and increased caspase 3 in the corpus cavernosum of rats, without altered serum and cavernosal miR-21 gene expression. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2020-06-03 /pmc/articles/PMC7282493/ /pubmed/32520081 http://dx.doi.org/10.1590/s0102-865020200030000005 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Original Article Meirelles, Rogério José de Azevedo Lizarte, Fermino Sanches Cirino, Mucio Luiz de Assis Novais, Paulo Cezar Gula, Isabella Stracieri da Silva, Jairo Pinheiro Tazzima, Maria de Fátima Galli Sorita Fazan, Valéria Paula Sassoli Durand, Marina Toledo Tirapelli, Daniela Pretti da Cunha de Carvalho, Camila Albuquerque Melo Schimming, Bruno César Molina, Carlos Augusto Fernandes Tucci, Silvio Tirapelli, Luis Fernando Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model |
| title | Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model
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| title_full | Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model
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| title_fullStr | Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model
|
| title_full_unstemmed | Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model
|
| title_short | Morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model
|
| title_sort | morphological and molecular analysis of apoptosis in the corpus cavernosum of rats submitted to a chronic alcoholism model |
| topic | Original Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7282493/ https://www.ncbi.nlm.nih.gov/pubmed/32520081 http://dx.doi.org/10.1590/s0102-865020200030000005 |
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