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Toward fine-tuned metabolic networks in industrial microorganisms

There are numerous microorganisms in nature capable of synthesizing diverse useful compounds; however, these natural microorganisms are generally inefficient in the production of target products on an industrial scale, relative to either chemical synthesis or extraction methods. To achieve industria...

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Detalles Bibliográficos
Autores principales: Li, Ning, Zeng, Weizhu, Xu, Sha, Zhou, Jingwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283098/
https://www.ncbi.nlm.nih.gov/pubmed/32542205
http://dx.doi.org/10.1016/j.synbio.2020.05.002
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author Li, Ning
Zeng, Weizhu
Xu, Sha
Zhou, Jingwen
author_facet Li, Ning
Zeng, Weizhu
Xu, Sha
Zhou, Jingwen
author_sort Li, Ning
collection PubMed
description There are numerous microorganisms in nature capable of synthesizing diverse useful compounds; however, these natural microorganisms are generally inefficient in the production of target products on an industrial scale, relative to either chemical synthesis or extraction methods. To achieve industrial production of useful compounds, these natural microorganisms must undergo a certain degree of mutation or effective fine-tuning strategies. This review describes how to achieve an ideal metabolic fine-tuned process, including static control strategies and dynamic control strategies. The static control strategies mainly focus on various matabolic engineering strategies, including protein engineering, upregulation/downregulation, and combinatrorial control of these metabolic engineering strategies, to enhance the flexibility of their application in fine-tuned metabolic metworks. Then, we focus on the dynamic control strategies for fine-tuned metabolic metworks. The design principles derived would guide us to construct microbial cell factories for various useful compounds.
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spelling pubmed-72830982020-06-14 Toward fine-tuned metabolic networks in industrial microorganisms Li, Ning Zeng, Weizhu Xu, Sha Zhou, Jingwen Synth Syst Biotechnol Article There are numerous microorganisms in nature capable of synthesizing diverse useful compounds; however, these natural microorganisms are generally inefficient in the production of target products on an industrial scale, relative to either chemical synthesis or extraction methods. To achieve industrial production of useful compounds, these natural microorganisms must undergo a certain degree of mutation or effective fine-tuning strategies. This review describes how to achieve an ideal metabolic fine-tuned process, including static control strategies and dynamic control strategies. The static control strategies mainly focus on various matabolic engineering strategies, including protein engineering, upregulation/downregulation, and combinatrorial control of these metabolic engineering strategies, to enhance the flexibility of their application in fine-tuned metabolic metworks. Then, we focus on the dynamic control strategies for fine-tuned metabolic metworks. The design principles derived would guide us to construct microbial cell factories for various useful compounds. KeAi Publishing 2020-06-05 /pmc/articles/PMC7283098/ /pubmed/32542205 http://dx.doi.org/10.1016/j.synbio.2020.05.002 Text en © 2020 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Li, Ning
Zeng, Weizhu
Xu, Sha
Zhou, Jingwen
Toward fine-tuned metabolic networks in industrial microorganisms
title Toward fine-tuned metabolic networks in industrial microorganisms
title_full Toward fine-tuned metabolic networks in industrial microorganisms
title_fullStr Toward fine-tuned metabolic networks in industrial microorganisms
title_full_unstemmed Toward fine-tuned metabolic networks in industrial microorganisms
title_short Toward fine-tuned metabolic networks in industrial microorganisms
title_sort toward fine-tuned metabolic networks in industrial microorganisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283098/
https://www.ncbi.nlm.nih.gov/pubmed/32542205
http://dx.doi.org/10.1016/j.synbio.2020.05.002
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