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Toward fine-tuned metabolic networks in industrial microorganisms
There are numerous microorganisms in nature capable of synthesizing diverse useful compounds; however, these natural microorganisms are generally inefficient in the production of target products on an industrial scale, relative to either chemical synthesis or extraction methods. To achieve industria...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283098/ https://www.ncbi.nlm.nih.gov/pubmed/32542205 http://dx.doi.org/10.1016/j.synbio.2020.05.002 |
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author | Li, Ning Zeng, Weizhu Xu, Sha Zhou, Jingwen |
author_facet | Li, Ning Zeng, Weizhu Xu, Sha Zhou, Jingwen |
author_sort | Li, Ning |
collection | PubMed |
description | There are numerous microorganisms in nature capable of synthesizing diverse useful compounds; however, these natural microorganisms are generally inefficient in the production of target products on an industrial scale, relative to either chemical synthesis or extraction methods. To achieve industrial production of useful compounds, these natural microorganisms must undergo a certain degree of mutation or effective fine-tuning strategies. This review describes how to achieve an ideal metabolic fine-tuned process, including static control strategies and dynamic control strategies. The static control strategies mainly focus on various matabolic engineering strategies, including protein engineering, upregulation/downregulation, and combinatrorial control of these metabolic engineering strategies, to enhance the flexibility of their application in fine-tuned metabolic metworks. Then, we focus on the dynamic control strategies for fine-tuned metabolic metworks. The design principles derived would guide us to construct microbial cell factories for various useful compounds. |
format | Online Article Text |
id | pubmed-7283098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-72830982020-06-14 Toward fine-tuned metabolic networks in industrial microorganisms Li, Ning Zeng, Weizhu Xu, Sha Zhou, Jingwen Synth Syst Biotechnol Article There are numerous microorganisms in nature capable of synthesizing diverse useful compounds; however, these natural microorganisms are generally inefficient in the production of target products on an industrial scale, relative to either chemical synthesis or extraction methods. To achieve industrial production of useful compounds, these natural microorganisms must undergo a certain degree of mutation or effective fine-tuning strategies. This review describes how to achieve an ideal metabolic fine-tuned process, including static control strategies and dynamic control strategies. The static control strategies mainly focus on various matabolic engineering strategies, including protein engineering, upregulation/downregulation, and combinatrorial control of these metabolic engineering strategies, to enhance the flexibility of their application in fine-tuned metabolic metworks. Then, we focus on the dynamic control strategies for fine-tuned metabolic metworks. The design principles derived would guide us to construct microbial cell factories for various useful compounds. KeAi Publishing 2020-06-05 /pmc/articles/PMC7283098/ /pubmed/32542205 http://dx.doi.org/10.1016/j.synbio.2020.05.002 Text en © 2020 Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Li, Ning Zeng, Weizhu Xu, Sha Zhou, Jingwen Toward fine-tuned metabolic networks in industrial microorganisms |
title | Toward fine-tuned metabolic networks in industrial microorganisms |
title_full | Toward fine-tuned metabolic networks in industrial microorganisms |
title_fullStr | Toward fine-tuned metabolic networks in industrial microorganisms |
title_full_unstemmed | Toward fine-tuned metabolic networks in industrial microorganisms |
title_short | Toward fine-tuned metabolic networks in industrial microorganisms |
title_sort | toward fine-tuned metabolic networks in industrial microorganisms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283098/ https://www.ncbi.nlm.nih.gov/pubmed/32542205 http://dx.doi.org/10.1016/j.synbio.2020.05.002 |
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