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Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus
Cadmium, although not redox active is highly toxic. Yet, the underlying mechanisms driving toxicity are still to be characterized. In this study, we took advantage of the purple bacterium Rubrivivax gelatinosus strain with defective Cd(2 +)-efflux system to identify targets of this metal. Exposure o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283390/ https://www.ncbi.nlm.nih.gov/pubmed/32582041 http://dx.doi.org/10.3389/fmicb.2020.00893 |
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author | Steunou, Anne Soisig Durand, Anne Bourbon, Marie-Line Babot, Marion Tambosi, Reem Liotenberg, Sylviane Ouchane, Soufian |
author_facet | Steunou, Anne Soisig Durand, Anne Bourbon, Marie-Line Babot, Marion Tambosi, Reem Liotenberg, Sylviane Ouchane, Soufian |
author_sort | Steunou, Anne Soisig |
collection | PubMed |
description | Cadmium, although not redox active is highly toxic. Yet, the underlying mechanisms driving toxicity are still to be characterized. In this study, we took advantage of the purple bacterium Rubrivivax gelatinosus strain with defective Cd(2 +)-efflux system to identify targets of this metal. Exposure of the ΔcadA strain to Cd(2 +) causes a decrease in the photosystem amount and in the activity of respiratory complexes. As in case of Cu(+) toxicity, the data indicated that Cd(2 +) targets the porphyrin biosynthesis pathway at the level of HemN, a S-adenosylmethionine and CxxxCxxC coordinated [4Fe-4S] containing enzyme. Cd(2 +) exposure therefore results in a deficiency in heme and chlorophyll dependent proteins and metabolic pathways. Given the importance of porphyrin biosynthesis, HemN represents a key metal target to account for toxicity. In the environment, microorganisms are exposed to mixture of metals. Nevertheless, the biological effects of such mixtures, and the toxicity mechanisms remain poorly addressed. To highlight a potential cross-talk between Cd(2 +) and Cu(+) -efflux systems, we show (i) that Cd(2 +) induces the expression of the Cd(2 +)-efflux pump CadA and the Cu(+) detoxification system CopA and CopI; and (ii) that Cu(+) ions improve tolerance towards Cd(2 +), demonstrating thus that metal mixtures could also represent a selective advantage in the environment. |
format | Online Article Text |
id | pubmed-7283390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72833902020-06-23 Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus Steunou, Anne Soisig Durand, Anne Bourbon, Marie-Line Babot, Marion Tambosi, Reem Liotenberg, Sylviane Ouchane, Soufian Front Microbiol Microbiology Cadmium, although not redox active is highly toxic. Yet, the underlying mechanisms driving toxicity are still to be characterized. In this study, we took advantage of the purple bacterium Rubrivivax gelatinosus strain with defective Cd(2 +)-efflux system to identify targets of this metal. Exposure of the ΔcadA strain to Cd(2 +) causes a decrease in the photosystem amount and in the activity of respiratory complexes. As in case of Cu(+) toxicity, the data indicated that Cd(2 +) targets the porphyrin biosynthesis pathway at the level of HemN, a S-adenosylmethionine and CxxxCxxC coordinated [4Fe-4S] containing enzyme. Cd(2 +) exposure therefore results in a deficiency in heme and chlorophyll dependent proteins and metabolic pathways. Given the importance of porphyrin biosynthesis, HemN represents a key metal target to account for toxicity. In the environment, microorganisms are exposed to mixture of metals. Nevertheless, the biological effects of such mixtures, and the toxicity mechanisms remain poorly addressed. To highlight a potential cross-talk between Cd(2 +) and Cu(+) -efflux systems, we show (i) that Cd(2 +) induces the expression of the Cd(2 +)-efflux pump CadA and the Cu(+) detoxification system CopA and CopI; and (ii) that Cu(+) ions improve tolerance towards Cd(2 +), demonstrating thus that metal mixtures could also represent a selective advantage in the environment. Frontiers Media S.A. 2020-06-03 /pmc/articles/PMC7283390/ /pubmed/32582041 http://dx.doi.org/10.3389/fmicb.2020.00893 Text en Copyright © 2020 Steunou, Durand, Bourbon, Babot, Tambosi, Liotenberg and Ouchane. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Steunou, Anne Soisig Durand, Anne Bourbon, Marie-Line Babot, Marion Tambosi, Reem Liotenberg, Sylviane Ouchane, Soufian Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus |
title | Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus |
title_full | Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus |
title_fullStr | Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus |
title_full_unstemmed | Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus |
title_short | Cadmium and Copper Cross-Tolerance. Cu(+) Alleviates Cd(2 +) Toxicity, and Both Cations Target Heme and Chlorophyll Biosynthesis Pathway in Rubrivivax gelatinosus |
title_sort | cadmium and copper cross-tolerance. cu(+) alleviates cd(2 +) toxicity, and both cations target heme and chlorophyll biosynthesis pathway in rubrivivax gelatinosus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283390/ https://www.ncbi.nlm.nih.gov/pubmed/32582041 http://dx.doi.org/10.3389/fmicb.2020.00893 |
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