Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer

Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin...

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Autores principales: Dortet, Laurent, Bonnin, Rémy A., Le Hello, Simon, Fabre, Laetitia, Bonnet, Richard, Kostrzewa, Markus, Filloux, Alain, Larrouy-Maumus, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283459/
https://www.ncbi.nlm.nih.gov/pubmed/32582090
http://dx.doi.org/10.3389/fmicb.2020.01141
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author Dortet, Laurent
Bonnin, Rémy A.
Le Hello, Simon
Fabre, Laetitia
Bonnet, Richard
Kostrzewa, Markus
Filloux, Alain
Larrouy-Maumus, Gerald
author_facet Dortet, Laurent
Bonnin, Rémy A.
Le Hello, Simon
Fabre, Laetitia
Bonnet, Richard
Kostrzewa, Markus
Filloux, Alain
Larrouy-Maumus, Gerald
author_sort Dortet, Laurent
collection PubMed
description Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 h) and challenging to perform in clinical and veterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant Salmonella enterica using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system.
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spelling pubmed-72834592020-06-23 Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer Dortet, Laurent Bonnin, Rémy A. Le Hello, Simon Fabre, Laetitia Bonnet, Richard Kostrzewa, Markus Filloux, Alain Larrouy-Maumus, Gerald Front Microbiol Microbiology Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 h) and challenging to perform in clinical and veterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant Salmonella enterica using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system. Frontiers Media S.A. 2020-06-03 /pmc/articles/PMC7283459/ /pubmed/32582090 http://dx.doi.org/10.3389/fmicb.2020.01141 Text en Copyright © 2020 Dortet, Bonnin, Le Hello, Fabre, Bonnet, Kostrzewa, Filloux and Larrouy-Maumus. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dortet, Laurent
Bonnin, Rémy A.
Le Hello, Simon
Fabre, Laetitia
Bonnet, Richard
Kostrzewa, Markus
Filloux, Alain
Larrouy-Maumus, Gerald
Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_full Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_fullStr Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_full_unstemmed Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_short Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_sort detection of colistin resistance in salmonella enterica using maldixin test on the routine maldi biotyper sirius mass spectrometer
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283459/
https://www.ncbi.nlm.nih.gov/pubmed/32582090
http://dx.doi.org/10.3389/fmicb.2020.01141
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