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Non-neuromodulatory Optogenetic Tools in Zebrafish

The zebrafish (Danio rerio) is a popular vertebrate model organism to investigate molecular mechanisms driving development and disease. Due to its transparency at embryonic and larval stages, investigations in the living organism are possible with subcellular resolution using intravital microscopy....

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Autores principales: Varady, Adam, Distel, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283495/
https://www.ncbi.nlm.nih.gov/pubmed/32582702
http://dx.doi.org/10.3389/fcell.2020.00418
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author Varady, Adam
Distel, Martin
author_facet Varady, Adam
Distel, Martin
author_sort Varady, Adam
collection PubMed
description The zebrafish (Danio rerio) is a popular vertebrate model organism to investigate molecular mechanisms driving development and disease. Due to its transparency at embryonic and larval stages, investigations in the living organism are possible with subcellular resolution using intravital microscopy. The beneficial optical characteristics of zebrafish not only allow for passive observation, but also active manipulation of proteins and cells by light using optogenetic tools. Initially, photosensitive ion channels have been applied for neurobiological studies in zebrafish to dissect complex behaviors on a cellular level. More recently, exciting non-neural optogenetic tools have been established to control gene expression or protein localization and activity, allowing for unprecedented non-invasive and precise manipulation of various aspects of cellular physiology. Zebrafish will likely be a vertebrate model organism at the forefront of in vivo application of non-neural optogenetic tools and pioneering work has already been performed. In this review, we provide an overview of non-neuromodulatory optogenetic tools successfully applied in zebrafish to control gene expression, protein localization, cell signaling, migration and cell ablation.
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spelling pubmed-72834952020-06-23 Non-neuromodulatory Optogenetic Tools in Zebrafish Varady, Adam Distel, Martin Front Cell Dev Biol Cell and Developmental Biology The zebrafish (Danio rerio) is a popular vertebrate model organism to investigate molecular mechanisms driving development and disease. Due to its transparency at embryonic and larval stages, investigations in the living organism are possible with subcellular resolution using intravital microscopy. The beneficial optical characteristics of zebrafish not only allow for passive observation, but also active manipulation of proteins and cells by light using optogenetic tools. Initially, photosensitive ion channels have been applied for neurobiological studies in zebrafish to dissect complex behaviors on a cellular level. More recently, exciting non-neural optogenetic tools have been established to control gene expression or protein localization and activity, allowing for unprecedented non-invasive and precise manipulation of various aspects of cellular physiology. Zebrafish will likely be a vertebrate model organism at the forefront of in vivo application of non-neural optogenetic tools and pioneering work has already been performed. In this review, we provide an overview of non-neuromodulatory optogenetic tools successfully applied in zebrafish to control gene expression, protein localization, cell signaling, migration and cell ablation. Frontiers Media S.A. 2020-06-03 /pmc/articles/PMC7283495/ /pubmed/32582702 http://dx.doi.org/10.3389/fcell.2020.00418 Text en Copyright © 2020 Varady and Distel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Varady, Adam
Distel, Martin
Non-neuromodulatory Optogenetic Tools in Zebrafish
title Non-neuromodulatory Optogenetic Tools in Zebrafish
title_full Non-neuromodulatory Optogenetic Tools in Zebrafish
title_fullStr Non-neuromodulatory Optogenetic Tools in Zebrafish
title_full_unstemmed Non-neuromodulatory Optogenetic Tools in Zebrafish
title_short Non-neuromodulatory Optogenetic Tools in Zebrafish
title_sort non-neuromodulatory optogenetic tools in zebrafish
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283495/
https://www.ncbi.nlm.nih.gov/pubmed/32582702
http://dx.doi.org/10.3389/fcell.2020.00418
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