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Non-neuromodulatory Optogenetic Tools in Zebrafish
The zebrafish (Danio rerio) is a popular vertebrate model organism to investigate molecular mechanisms driving development and disease. Due to its transparency at embryonic and larval stages, investigations in the living organism are possible with subcellular resolution using intravital microscopy....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283495/ https://www.ncbi.nlm.nih.gov/pubmed/32582702 http://dx.doi.org/10.3389/fcell.2020.00418 |
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author | Varady, Adam Distel, Martin |
author_facet | Varady, Adam Distel, Martin |
author_sort | Varady, Adam |
collection | PubMed |
description | The zebrafish (Danio rerio) is a popular vertebrate model organism to investigate molecular mechanisms driving development and disease. Due to its transparency at embryonic and larval stages, investigations in the living organism are possible with subcellular resolution using intravital microscopy. The beneficial optical characteristics of zebrafish not only allow for passive observation, but also active manipulation of proteins and cells by light using optogenetic tools. Initially, photosensitive ion channels have been applied for neurobiological studies in zebrafish to dissect complex behaviors on a cellular level. More recently, exciting non-neural optogenetic tools have been established to control gene expression or protein localization and activity, allowing for unprecedented non-invasive and precise manipulation of various aspects of cellular physiology. Zebrafish will likely be a vertebrate model organism at the forefront of in vivo application of non-neural optogenetic tools and pioneering work has already been performed. In this review, we provide an overview of non-neuromodulatory optogenetic tools successfully applied in zebrafish to control gene expression, protein localization, cell signaling, migration and cell ablation. |
format | Online Article Text |
id | pubmed-7283495 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72834952020-06-23 Non-neuromodulatory Optogenetic Tools in Zebrafish Varady, Adam Distel, Martin Front Cell Dev Biol Cell and Developmental Biology The zebrafish (Danio rerio) is a popular vertebrate model organism to investigate molecular mechanisms driving development and disease. Due to its transparency at embryonic and larval stages, investigations in the living organism are possible with subcellular resolution using intravital microscopy. The beneficial optical characteristics of zebrafish not only allow for passive observation, but also active manipulation of proteins and cells by light using optogenetic tools. Initially, photosensitive ion channels have been applied for neurobiological studies in zebrafish to dissect complex behaviors on a cellular level. More recently, exciting non-neural optogenetic tools have been established to control gene expression or protein localization and activity, allowing for unprecedented non-invasive and precise manipulation of various aspects of cellular physiology. Zebrafish will likely be a vertebrate model organism at the forefront of in vivo application of non-neural optogenetic tools and pioneering work has already been performed. In this review, we provide an overview of non-neuromodulatory optogenetic tools successfully applied in zebrafish to control gene expression, protein localization, cell signaling, migration and cell ablation. Frontiers Media S.A. 2020-06-03 /pmc/articles/PMC7283495/ /pubmed/32582702 http://dx.doi.org/10.3389/fcell.2020.00418 Text en Copyright © 2020 Varady and Distel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Varady, Adam Distel, Martin Non-neuromodulatory Optogenetic Tools in Zebrafish |
title | Non-neuromodulatory Optogenetic Tools in Zebrafish |
title_full | Non-neuromodulatory Optogenetic Tools in Zebrafish |
title_fullStr | Non-neuromodulatory Optogenetic Tools in Zebrafish |
title_full_unstemmed | Non-neuromodulatory Optogenetic Tools in Zebrafish |
title_short | Non-neuromodulatory Optogenetic Tools in Zebrafish |
title_sort | non-neuromodulatory optogenetic tools in zebrafish |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283495/ https://www.ncbi.nlm.nih.gov/pubmed/32582702 http://dx.doi.org/10.3389/fcell.2020.00418 |
work_keys_str_mv | AT varadyadam nonneuromodulatoryoptogenetictoolsinzebrafish AT distelmartin nonneuromodulatoryoptogenetictoolsinzebrafish |