Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli

Assembly-line polyketide synthases generate natural products that have led to many live-saving drugs. The use of E. coli as a heterologous host for reconstituting these enormous and complex enzymatic machines has and will continue to be a critical strategy for understanding them. Here, we concisely...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuet, Kai P., Khosla, Chaitan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Special issue on The Natural Product Issue edited by Greg Stephanopoulos, Anthony Sinskey and Kang Zhou
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283498/
https://www.ncbi.nlm.nih.gov/pubmed/32547924
http://dx.doi.org/10.1016/j.mec.2019.e00106
Descripción
Sumario:Assembly-line polyketide synthases generate natural products that have led to many live-saving drugs. The use of E. coli as a heterologous host for reconstituting these enormous and complex enzymatic machines has and will continue to be a critical strategy for understanding them. Here, we concisely summarize successful examples in exploiting E. coli for assembly-line polyketide biosynthesis as well as offer examples of new challenges in which this approach is primed to tackle.

Ejemplares similares