Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli

Assembly-line polyketide synthases generate natural products that have led to many live-saving drugs. The use of E. coli as a heterologous host for reconstituting these enormous and complex enzymatic machines has and will continue to be a critical strategy for understanding them. Here, we concisely...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuet, Kai P., Khosla, Chaitan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Special issue on The Natural Product Issue edited by Greg Stephanopoulos, Anthony Sinskey and Kang Zhou
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283498/
https://www.ncbi.nlm.nih.gov/pubmed/32547924
http://dx.doi.org/10.1016/j.mec.2019.e00106
_version_ 1783544312724717568
author Yuet, Kai P.
Khosla, Chaitan
author_facet Yuet, Kai P.
Khosla, Chaitan
author_sort Yuet, Kai P.
collection PubMed
description Assembly-line polyketide synthases generate natural products that have led to many live-saving drugs. The use of E. coli as a heterologous host for reconstituting these enormous and complex enzymatic machines has and will continue to be a critical strategy for understanding them. Here, we concisely summarize successful examples in exploiting E. coli for assembly-line polyketide biosynthesis as well as offer examples of new challenges in which this approach is primed to tackle.
format Online
Article
Text
id pubmed-7283498
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-72834982020-06-15 Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli Yuet, Kai P. Khosla, Chaitan Metab Eng Commun Special issue on The Natural Product Issue edited by Greg Stephanopoulos, Anthony Sinskey and Kang Zhou Assembly-line polyketide synthases generate natural products that have led to many live-saving drugs. The use of E. coli as a heterologous host for reconstituting these enormous and complex enzymatic machines has and will continue to be a critical strategy for understanding them. Here, we concisely summarize successful examples in exploiting E. coli for assembly-line polyketide biosynthesis as well as offer examples of new challenges in which this approach is primed to tackle. Elsevier 2019-11-01 /pmc/articles/PMC7283498/ /pubmed/32547924 http://dx.doi.org/10.1016/j.mec.2019.e00106 Text en © 2019 Published by Elsevier B.V. on behalf of International Metabolic Engineering Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Special issue on The Natural Product Issue edited by Greg Stephanopoulos, Anthony Sinskey and Kang Zhou
Yuet, Kai P.
Khosla, Chaitan
Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli
title Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli
title_full Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli
title_fullStr Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli
title_full_unstemmed Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli
title_short Challenges and opportunities for engineering assembly-line polyketide biosynthesis in Escherichia coli
title_sort challenges and opportunities for engineering assembly-line polyketide biosynthesis in escherichia coli
topic Special issue on The Natural Product Issue edited by Greg Stephanopoulos, Anthony Sinskey and Kang Zhou
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283498/
https://www.ncbi.nlm.nih.gov/pubmed/32547924
http://dx.doi.org/10.1016/j.mec.2019.e00106
work_keys_str_mv AT yuetkaip challengesandopportunitiesforengineeringassemblylinepolyketidebiosynthesisinescherichiacoli
AT khoslachaitan challengesandopportunitiesforengineeringassemblylinepolyketidebiosynthesisinescherichiacoli

Ejemplares similares