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Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability

Crop contamination by soil-borne pathogenic microorganisms often leads to serious infection outbreaks. Plant protection requires disinfection of agricultural lands. The chemical and the physical disinfection procedures have several disadvantages, including an irreversible change in the soil ecosyste...

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Autores principales: Lazra, Yulia, Dubrovin, Irina, Multanen, Victor, Bormashenko, Edward, Bormashenko, Yelena, Cahan, Rivka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284381/
https://www.ncbi.nlm.nih.gov/pubmed/32403235
http://dx.doi.org/10.3390/microorganisms8050704
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author Lazra, Yulia
Dubrovin, Irina
Multanen, Victor
Bormashenko, Edward
Bormashenko, Yelena
Cahan, Rivka
author_facet Lazra, Yulia
Dubrovin, Irina
Multanen, Victor
Bormashenko, Edward
Bormashenko, Yelena
Cahan, Rivka
author_sort Lazra, Yulia
collection PubMed
description Crop contamination by soil-borne pathogenic microorganisms often leads to serious infection outbreaks. Plant protection requires disinfection of agricultural lands. The chemical and the physical disinfection procedures have several disadvantages, including an irreversible change in the soil ecosystem. Plasma, the “fourth state of matter” is defined as an ionized gas containing an equal number of negatively and positively charged particles. Cold-plasma technology with air or oxygen as the working gas generates reactive oxygen species, which are found to efficiently eradicate bacteria. In this study, we examined the effect of atmospheric plasma corona discharges on soil bacteria viability. Soil that was exposed to plasma for 60 s resulted in bacterial reduction by two orders of magnitude, from 1.1 × 10(5) to 2.3 × 10(3) cells g(−1) soil. Exposure for a longer period of 5 min did not lead to further significant reduction in bacterial concentration (a final reduction of only 2.5 orders of magnitude). The bacterial viability was evaluated using a colorimetric assay based on the bacterial hydrogenases immediately after exposure and at selected times during 24 h. The result showed no recovery in the bacterial viability. Plasma discharged directly on bacteria that were isolated from the soil resulted in a reduction by four orders of magnitude in the bacterial concentration compared to untreated isolated bacteria: 2.6 × 10(−3) and 1.7 × 10(−7), respectively. The plasma-resistant bacteria were found to be related to the taxonomic phylum Firmicutes (98.5%) and comprised the taxonomic orders Bacillales (95%) and Clostridiales (2%). To our knowledge, this is the first study of soil bacteria eradication using plasma corona discharges.
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spelling pubmed-72843812020-08-13 Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability Lazra, Yulia Dubrovin, Irina Multanen, Victor Bormashenko, Edward Bormashenko, Yelena Cahan, Rivka Microorganisms Article Crop contamination by soil-borne pathogenic microorganisms often leads to serious infection outbreaks. Plant protection requires disinfection of agricultural lands. The chemical and the physical disinfection procedures have several disadvantages, including an irreversible change in the soil ecosystem. Plasma, the “fourth state of matter” is defined as an ionized gas containing an equal number of negatively and positively charged particles. Cold-plasma technology with air or oxygen as the working gas generates reactive oxygen species, which are found to efficiently eradicate bacteria. In this study, we examined the effect of atmospheric plasma corona discharges on soil bacteria viability. Soil that was exposed to plasma for 60 s resulted in bacterial reduction by two orders of magnitude, from 1.1 × 10(5) to 2.3 × 10(3) cells g(−1) soil. Exposure for a longer period of 5 min did not lead to further significant reduction in bacterial concentration (a final reduction of only 2.5 orders of magnitude). The bacterial viability was evaluated using a colorimetric assay based on the bacterial hydrogenases immediately after exposure and at selected times during 24 h. The result showed no recovery in the bacterial viability. Plasma discharged directly on bacteria that were isolated from the soil resulted in a reduction by four orders of magnitude in the bacterial concentration compared to untreated isolated bacteria: 2.6 × 10(−3) and 1.7 × 10(−7), respectively. The plasma-resistant bacteria were found to be related to the taxonomic phylum Firmicutes (98.5%) and comprised the taxonomic orders Bacillales (95%) and Clostridiales (2%). To our knowledge, this is the first study of soil bacteria eradication using plasma corona discharges. MDPI 2020-05-11 /pmc/articles/PMC7284381/ /pubmed/32403235 http://dx.doi.org/10.3390/microorganisms8050704 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lazra, Yulia
Dubrovin, Irina
Multanen, Victor
Bormashenko, Edward
Bormashenko, Yelena
Cahan, Rivka
Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability
title Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability
title_full Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability
title_fullStr Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability
title_full_unstemmed Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability
title_short Effects of Atmospheric Plasma Corona Discharges on Soil Bacteria Viability
title_sort effects of atmospheric plasma corona discharges on soil bacteria viability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284381/
https://www.ncbi.nlm.nih.gov/pubmed/32403235
http://dx.doi.org/10.3390/microorganisms8050704
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