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Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia

Silicon (Si) is a beneficial element for plants. To understand Si uptake and accumulation in poinsettia, the Si transporters and their expression patterns were investigated. Nodulin 26-like intrinsic membrane proteins (NIPs) act as transporters of water and small solutes, including silicic acid. In...

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Autores principales: Hu, Jiangtao, Li, Yali, Jeong, Byoung Ryong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284485/
https://www.ncbi.nlm.nih.gov/pubmed/32365688
http://dx.doi.org/10.3390/plants9050569
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author Hu, Jiangtao
Li, Yali
Jeong, Byoung Ryong
author_facet Hu, Jiangtao
Li, Yali
Jeong, Byoung Ryong
author_sort Hu, Jiangtao
collection PubMed
description Silicon (Si) is a beneficial element for plants. To understand Si uptake and accumulation in poinsettia, the Si transporters and their expression patterns were investigated. Nodulin 26-like intrinsic membrane proteins (NIPs) act as transporters of water and small solutes, including silicic acid. In this study, one NIP member, designated EpLsi1, was identified. Additionally, a protein from the citrate transporter family, designated EpLsi2, was identified. Sequence analyses indicated that EpLsi1 belonged to the NIP-I subgroup, which has a low Si uptake capacity. Consistently, the measured tissue Si content in the poinsettia was less than 1.73 ± 0.17 mg·g(−1) dry weight, which was very low when compared to that in high Si accumulators. The expressions of EpLsi1 and EpLsi2 in poinsettia cuttings treated with 0 mg·L(−1) Si decreased under temperature stresses. A short-term Si supplementation decreased the expressions of both EpLsi1 and EpLsi2 in the roots and leaves, while a long-term Si supplementation increased the expression of EpLsi1 in the leaves, bracts, and cyathia, and increased the expression of EpLsi2 in the roots and leaves. Tissue Si content increased in the roots of cuttings treated with 75 mg·L(−1) Si at both 4 and 40 °C, indicating that the transport activities of the EpLsi1 were enhanced under temperature stresses. A long-term Si supplementation increased the tissue Si content in the roots of poinsettia treated with 75 mg·L(−1) Si. Overall, poinsettia was a low Si accumulator, the expressions of Si transporters were down-regulated, and the tissue Si content increased with temperature stresses and Si supplementation. These results may help the breeding and commercial production of poinsettia.
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spelling pubmed-72844852020-06-19 Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia Hu, Jiangtao Li, Yali Jeong, Byoung Ryong Plants (Basel) Article Silicon (Si) is a beneficial element for plants. To understand Si uptake and accumulation in poinsettia, the Si transporters and their expression patterns were investigated. Nodulin 26-like intrinsic membrane proteins (NIPs) act as transporters of water and small solutes, including silicic acid. In this study, one NIP member, designated EpLsi1, was identified. Additionally, a protein from the citrate transporter family, designated EpLsi2, was identified. Sequence analyses indicated that EpLsi1 belonged to the NIP-I subgroup, which has a low Si uptake capacity. Consistently, the measured tissue Si content in the poinsettia was less than 1.73 ± 0.17 mg·g(−1) dry weight, which was very low when compared to that in high Si accumulators. The expressions of EpLsi1 and EpLsi2 in poinsettia cuttings treated with 0 mg·L(−1) Si decreased under temperature stresses. A short-term Si supplementation decreased the expressions of both EpLsi1 and EpLsi2 in the roots and leaves, while a long-term Si supplementation increased the expression of EpLsi1 in the leaves, bracts, and cyathia, and increased the expression of EpLsi2 in the roots and leaves. Tissue Si content increased in the roots of cuttings treated with 75 mg·L(−1) Si at both 4 and 40 °C, indicating that the transport activities of the EpLsi1 were enhanced under temperature stresses. A long-term Si supplementation increased the tissue Si content in the roots of poinsettia treated with 75 mg·L(−1) Si. Overall, poinsettia was a low Si accumulator, the expressions of Si transporters were down-regulated, and the tissue Si content increased with temperature stresses and Si supplementation. These results may help the breeding and commercial production of poinsettia. MDPI 2020-04-29 /pmc/articles/PMC7284485/ /pubmed/32365688 http://dx.doi.org/10.3390/plants9050569 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Jiangtao
Li, Yali
Jeong, Byoung Ryong
Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia
title Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia
title_full Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia
title_fullStr Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia
title_full_unstemmed Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia
title_short Putative Silicon Transporters and Effect of Temperature Stresses and Silicon Supplementation on Their Expressions and Tissue Silicon Content in Poinsettia
title_sort putative silicon transporters and effect of temperature stresses and silicon supplementation on their expressions and tissue silicon content in poinsettia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284485/
https://www.ncbi.nlm.nih.gov/pubmed/32365688
http://dx.doi.org/10.3390/plants9050569
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