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Insecticidal Activity of Hyoscyamus niger L. on Lucilia sericata Causing Myiasis

Background: Hyoscyamus niger L. (Solanaceae) generally known as henbane, is commonly distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk medicine to remove worms from the eyes. The present study aimed to investigate the insecticidal activity of H. niger seeds. Methods: n-...

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Detalles Bibliográficos
Autores principales: Küpeli Akkol, Esra, Ilhan, Mert, Kozan, Esma, Gürağaç Dereli, Fatma Tuğçe, Sak, Mustafa, Sobarzo-Sánchez, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284550/
https://www.ncbi.nlm.nih.gov/pubmed/32455988
http://dx.doi.org/10.3390/plants9050655
Descripción
Sumario:Background: Hyoscyamus niger L. (Solanaceae) generally known as henbane, is commonly distributed in Europe and Asia. In Turkey, henbane seeds have been used in folk medicine to remove worms from the eyes. The present study aimed to investigate the insecticidal activity of H. niger seeds. Methods: n-hexane, ethyl acetate, methanol and alkaloid extracts were prepared from the seeds of the plant and their insecticidal activities on Lucilia sericata larvae were evaluated. EC(50) and EC(90) values of the alkaloid extract were calculated and morphological abnormalities were investigated. Results: Alkaloid extract prepared from the seeds of this plant displayed significant insecticidal activity. EC(50) values of H. niger seeds alkaloid extract were found to be 8.04, 8.49, 7.96 μg/mL against first, second and third instar, respectively. It was determined that malformations of larvae included damaged larvae with small size, contraction and weak cuticle. Furthermore, HPLC analysis was performed on alkaloid extract of H. niger seeds and main components of the extract were determined. It was determined that alkaloid extract mainly contain hyoscyamine and scopolamine. Conclusions: These results confirm the folkloric usage of the plant and suggest that the alkaloid content of the plant could be responsible for the insecticidal activity.