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Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts

Nephelium lappaceum (rambutan) is an edible tropical fruit that is widely grown in Southeast Asia. In general, the seeds contain high nutrients, but rambutan seeds are thrown out during processing. In this study, the anti-aging activity of rambutan seeds was evaluated with a new approach through the...

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Autores principales: Lee, Yae Rin, Cho, Hyo Moon, Park, Eun Jin, Zhang, Mi, Doan, Thi Phuong, Lee, Ba Wool, Cho, Kyung A, Oh, Won Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284571/
https://www.ncbi.nlm.nih.gov/pubmed/32429147
http://dx.doi.org/10.3390/nu12051430
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author Lee, Yae Rin
Cho, Hyo Moon
Park, Eun Jin
Zhang, Mi
Doan, Thi Phuong
Lee, Ba Wool
Cho, Kyung A
Oh, Won Keun
author_facet Lee, Yae Rin
Cho, Hyo Moon
Park, Eun Jin
Zhang, Mi
Doan, Thi Phuong
Lee, Ba Wool
Cho, Kyung A
Oh, Won Keun
author_sort Lee, Yae Rin
collection PubMed
description Nephelium lappaceum (rambutan) is an edible tropical fruit that is widely grown in Southeast Asia. In general, the seeds contain high nutrients, but rambutan seeds are thrown out during processing. In this study, the anti-aging activity of rambutan seeds was evaluated with a new approach through the selective inhibition of the senescence-associated secretory phenotype (senomorphics). Luciferase promoter assays using p16INK4A and SA-β-gal promoters for rambutan showed that its seeds possessed strong senomorphic activity. Molecular networking using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-qTOF-MS) with a tandem database (UPLC-qTOF-MS/MS) was applied to determine the chemical composition of rambutan. Based on the activity results, nine compounds, one new (7) and eight known kaempferol type compounds, were isolated from the seeds. Compounds 2, 4 and 9 significantly reduced the mRNA expression levels of senescence markers, such as p16INK4A, p21CIP1, p53 and SA-β-gal. These compounds also significantly increased the level of SIRT1, a longevity modulator. Compounds 2, 4 and 9 decreased the mRNA expression levels of senescence-associated secretory phenotypes (SASPs) and subsequently decreased the number of SA-β-gal-positive cells. Thus, rambutan seeds and its constituents might be able to protect against age-related problems.
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spelling pubmed-72845712020-06-19 Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts Lee, Yae Rin Cho, Hyo Moon Park, Eun Jin Zhang, Mi Doan, Thi Phuong Lee, Ba Wool Cho, Kyung A Oh, Won Keun Nutrients Article Nephelium lappaceum (rambutan) is an edible tropical fruit that is widely grown in Southeast Asia. In general, the seeds contain high nutrients, but rambutan seeds are thrown out during processing. In this study, the anti-aging activity of rambutan seeds was evaluated with a new approach through the selective inhibition of the senescence-associated secretory phenotype (senomorphics). Luciferase promoter assays using p16INK4A and SA-β-gal promoters for rambutan showed that its seeds possessed strong senomorphic activity. Molecular networking using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-qTOF-MS) with a tandem database (UPLC-qTOF-MS/MS) was applied to determine the chemical composition of rambutan. Based on the activity results, nine compounds, one new (7) and eight known kaempferol type compounds, were isolated from the seeds. Compounds 2, 4 and 9 significantly reduced the mRNA expression levels of senescence markers, such as p16INK4A, p21CIP1, p53 and SA-β-gal. These compounds also significantly increased the level of SIRT1, a longevity modulator. Compounds 2, 4 and 9 decreased the mRNA expression levels of senescence-associated secretory phenotypes (SASPs) and subsequently decreased the number of SA-β-gal-positive cells. Thus, rambutan seeds and its constituents might be able to protect against age-related problems. MDPI 2020-05-15 /pmc/articles/PMC7284571/ /pubmed/32429147 http://dx.doi.org/10.3390/nu12051430 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Yae Rin
Cho, Hyo Moon
Park, Eun Jin
Zhang, Mi
Doan, Thi Phuong
Lee, Ba Wool
Cho, Kyung A
Oh, Won Keun
Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts
title Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts
title_full Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts
title_fullStr Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts
title_full_unstemmed Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts
title_short Metabolite Profiling of Rambutan (Nephelium lappaceum L.) Seeds Using UPLC-qTOF-MS/MS and Senomorphic Effects in Aged Human Dermal Fibroblasts
title_sort metabolite profiling of rambutan (nephelium lappaceum l.) seeds using uplc-qtof-ms/ms and senomorphic effects in aged human dermal fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284571/
https://www.ncbi.nlm.nih.gov/pubmed/32429147
http://dx.doi.org/10.3390/nu12051430
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