Cargando…
Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice
Sesamol found in sesame oil has been shown to ameliorate obesity by regulating lipid metabolism. However, its effects on energy expenditure and the underlying molecular mechanism have not been clearly elucidated. In this study, we show that sesamol increased the uncoupling protein 1 (Ucp1) expressio...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284577/ https://www.ncbi.nlm.nih.gov/pubmed/32443555 http://dx.doi.org/10.3390/nu12051459 |
_version_ | 1783544499777044480 |
---|---|
author | Lee, Dong Ho Chang, Seo-Hyuk Yang, Dong Kwon Song, No-Joon Yun, Ui Jeong Park, Kye Won |
author_facet | Lee, Dong Ho Chang, Seo-Hyuk Yang, Dong Kwon Song, No-Joon Yun, Ui Jeong Park, Kye Won |
author_sort | Lee, Dong Ho |
collection | PubMed |
description | Sesamol found in sesame oil has been shown to ameliorate obesity by regulating lipid metabolism. However, its effects on energy expenditure and the underlying molecular mechanism have not been clearly elucidated. In this study, we show that sesamol increased the uncoupling protein 1 (Ucp1) expression in adipocytes. The administration of sesamol in high-fat diet (HFD)-fed mice prevented weight gain and improved metabolic derangements. The three-week sesamol treatment of HFD-fed mice, when the body weights were not different between the sesamol and control groups, increased energy expenditure, suggesting that an induced energy expenditure is a primary contributing factor for sesamol’s anti-obese effects. Consistently, sesamol induced the expression of energy-dissipating thermogenic genes, including Ucp1, in white adipose tissues. The microarray analysis showed that sesamol dramatically increased the Nrf2 target genes such as Hmox1 and Atf3 in adipocytes. Moreover, 76% (60/79 genes) of the sesamol-induced genes were also regulated by tert-butylhydroquinone (tBHQ), a known Nrf2 activator. We further verified that sesamol directly activated the Nrf2-mediated transcription. In addition, the Hmox1 and Ucp1 induction by sesamol was compromised in Nrf2-deleted cells, indicating the necessity of Nrf2 in the sesamol-mediated Ucp1 induction. Together, these findings demonstrate the effects of sesamol in inducing Ucp1 and in increasing energy expenditure, further highlighting the use of the Nrf2 activation in stimulating thermogenic adipocytes and in increasing energy expenditure in obesity and its related metabolic diseases. |
format | Online Article Text |
id | pubmed-7284577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72845772020-06-19 Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice Lee, Dong Ho Chang, Seo-Hyuk Yang, Dong Kwon Song, No-Joon Yun, Ui Jeong Park, Kye Won Nutrients Article Sesamol found in sesame oil has been shown to ameliorate obesity by regulating lipid metabolism. However, its effects on energy expenditure and the underlying molecular mechanism have not been clearly elucidated. In this study, we show that sesamol increased the uncoupling protein 1 (Ucp1) expression in adipocytes. The administration of sesamol in high-fat diet (HFD)-fed mice prevented weight gain and improved metabolic derangements. The three-week sesamol treatment of HFD-fed mice, when the body weights were not different between the sesamol and control groups, increased energy expenditure, suggesting that an induced energy expenditure is a primary contributing factor for sesamol’s anti-obese effects. Consistently, sesamol induced the expression of energy-dissipating thermogenic genes, including Ucp1, in white adipose tissues. The microarray analysis showed that sesamol dramatically increased the Nrf2 target genes such as Hmox1 and Atf3 in adipocytes. Moreover, 76% (60/79 genes) of the sesamol-induced genes were also regulated by tert-butylhydroquinone (tBHQ), a known Nrf2 activator. We further verified that sesamol directly activated the Nrf2-mediated transcription. In addition, the Hmox1 and Ucp1 induction by sesamol was compromised in Nrf2-deleted cells, indicating the necessity of Nrf2 in the sesamol-mediated Ucp1 induction. Together, these findings demonstrate the effects of sesamol in inducing Ucp1 and in increasing energy expenditure, further highlighting the use of the Nrf2 activation in stimulating thermogenic adipocytes and in increasing energy expenditure in obesity and its related metabolic diseases. MDPI 2020-05-18 /pmc/articles/PMC7284577/ /pubmed/32443555 http://dx.doi.org/10.3390/nu12051459 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Dong Ho Chang, Seo-Hyuk Yang, Dong Kwon Song, No-Joon Yun, Ui Jeong Park, Kye Won Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice |
title | Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice |
title_full | Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice |
title_fullStr | Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice |
title_full_unstemmed | Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice |
title_short | Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice |
title_sort | sesamol increases ucp1 expression in white adipose tissues and stimulates energy expenditure in high-fat diet-fed obese mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284577/ https://www.ncbi.nlm.nih.gov/pubmed/32443555 http://dx.doi.org/10.3390/nu12051459 |
work_keys_str_mv | AT leedongho sesamolincreasesucp1expressioninwhiteadiposetissuesandstimulatesenergyexpenditureinhighfatdietfedobesemice AT changseohyuk sesamolincreasesucp1expressioninwhiteadiposetissuesandstimulatesenergyexpenditureinhighfatdietfedobesemice AT yangdongkwon sesamolincreasesucp1expressioninwhiteadiposetissuesandstimulatesenergyexpenditureinhighfatdietfedobesemice AT songnojoon sesamolincreasesucp1expressioninwhiteadiposetissuesandstimulatesenergyexpenditureinhighfatdietfedobesemice AT yunuijeong sesamolincreasesucp1expressioninwhiteadiposetissuesandstimulatesenergyexpenditureinhighfatdietfedobesemice AT parkkyewon sesamolincreasesucp1expressioninwhiteadiposetissuesandstimulatesenergyexpenditureinhighfatdietfedobesemice |