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Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548

Talaromyces albobiverticillius 30548 is a marine-derived pigment producing filamentous fungus, isolated from the La Réunion island, in the Indian Ocean. The objective of this study was to examine and optimize the submerged fermentation (SmF) process parameters such as initial pH (4–9), temperature (...

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Autores principales: Venkatachalam, Mekala, Shum-Chéong-Sing, Alain, Dufossé, Laurent, Fouillaud, Mireille
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284600/
https://www.ncbi.nlm.nih.gov/pubmed/32403428
http://dx.doi.org/10.3390/microorganisms8050711
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author Venkatachalam, Mekala
Shum-Chéong-Sing, Alain
Dufossé, Laurent
Fouillaud, Mireille
author_facet Venkatachalam, Mekala
Shum-Chéong-Sing, Alain
Dufossé, Laurent
Fouillaud, Mireille
author_sort Venkatachalam, Mekala
collection PubMed
description Talaromyces albobiverticillius 30548 is a marine-derived pigment producing filamentous fungus, isolated from the La Réunion island, in the Indian Ocean. The objective of this study was to examine and optimize the submerged fermentation (SmF) process parameters such as initial pH (4–9), temperature (21–27 °C), agitation speed (100–200 rpm), and fermentation time (0–336 h), for maximum production of pigments (orange and red) and biomass, using the Box–Behnken Experimental Design and Response Surface Modeling (BBED and RSM). This methodology allowed consideration of multifactorial interactions between a set of parameters. Experiments were carried out based on the BBED using 250 mL shake flasks, with a 100 mL working volume of potato dextrose broth (PDB). From the experimental data, mathematical models were developed to predict the pigments and biomass yields. The individual and interactive effects of the process variables on the responses were also investigated (RSM). The optimal conditions for maximum production of pigments and biomass were derived by the numerical optimization method, as follows—initial pH of 6.4, temperature of 24 °C, agitation speed of 164 rpm, and fermentation time of 149 h, respectively.
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spelling pubmed-72846002020-06-19 Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548 Venkatachalam, Mekala Shum-Chéong-Sing, Alain Dufossé, Laurent Fouillaud, Mireille Microorganisms Article Talaromyces albobiverticillius 30548 is a marine-derived pigment producing filamentous fungus, isolated from the La Réunion island, in the Indian Ocean. The objective of this study was to examine and optimize the submerged fermentation (SmF) process parameters such as initial pH (4–9), temperature (21–27 °C), agitation speed (100–200 rpm), and fermentation time (0–336 h), for maximum production of pigments (orange and red) and biomass, using the Box–Behnken Experimental Design and Response Surface Modeling (BBED and RSM). This methodology allowed consideration of multifactorial interactions between a set of parameters. Experiments were carried out based on the BBED using 250 mL shake flasks, with a 100 mL working volume of potato dextrose broth (PDB). From the experimental data, mathematical models were developed to predict the pigments and biomass yields. The individual and interactive effects of the process variables on the responses were also investigated (RSM). The optimal conditions for maximum production of pigments and biomass were derived by the numerical optimization method, as follows—initial pH of 6.4, temperature of 24 °C, agitation speed of 164 rpm, and fermentation time of 149 h, respectively. MDPI 2020-05-11 /pmc/articles/PMC7284600/ /pubmed/32403428 http://dx.doi.org/10.3390/microorganisms8050711 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Venkatachalam, Mekala
Shum-Chéong-Sing, Alain
Dufossé, Laurent
Fouillaud, Mireille
Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548
title Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548
title_full Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548
title_fullStr Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548
title_full_unstemmed Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548
title_short Statistical Optimization of the Physico-Chemical Parameters for Pigment Production in Submerged Fermentation of Talaromyces albobiverticillius 30548
title_sort statistical optimization of the physico-chemical parameters for pigment production in submerged fermentation of talaromyces albobiverticillius 30548
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284600/
https://www.ncbi.nlm.nih.gov/pubmed/32403428
http://dx.doi.org/10.3390/microorganisms8050711
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