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Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation

The objective of this research was to evaluate the PGPR effect on nodulation and nitrogen-fixing efficiency of soybean (Glycine max (L.) Merr.) by co-inoculation with Bradyrhizobium diazoefficiens USDA110. Co-inoculation of Bacillus velezensis S141 with USDA110 into soybean resulted in enhanced nodu...

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Autores principales: Sibponkrung, Surachat, Kondo, Takahiko, Tanaka, Kosei, Tittabutr, Panlada, Boonkerd, Nantakorn, Yoshida, Ken-ichi, Teaumroong, Neung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284691/
https://www.ncbi.nlm.nih.gov/pubmed/32392716
http://dx.doi.org/10.3390/microorganisms8050678
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author Sibponkrung, Surachat
Kondo, Takahiko
Tanaka, Kosei
Tittabutr, Panlada
Boonkerd, Nantakorn
Yoshida, Ken-ichi
Teaumroong, Neung
author_facet Sibponkrung, Surachat
Kondo, Takahiko
Tanaka, Kosei
Tittabutr, Panlada
Boonkerd, Nantakorn
Yoshida, Ken-ichi
Teaumroong, Neung
author_sort Sibponkrung, Surachat
collection PubMed
description The objective of this research was to evaluate the PGPR effect on nodulation and nitrogen-fixing efficiency of soybean (Glycine max (L.) Merr.) by co-inoculation with Bradyrhizobium diazoefficiens USDA110. Co-inoculation of Bacillus velezensis S141 with USDA110 into soybean resulted in enhanced nodulation and N2-fixing efficiency by producing larger nodules. To understand the role of S141 on soybean and USDA110 symbiosis, putative genes related to IAA biosynthesis were disrupted, suggesting that co-inoculation of USDA110 with S141ΔyhcX reduces the number of large size nodules. It was revealed that yhcX may play a major role in IAA biosynthesis in S141 as well as provide a major impact on soybean growth promotion. The disruption of genes related to cytokinin biosynthesis and co-inoculation of USDA110 with S141ΔIPI reduced the number of very large size nodules, and it appears that IPI might play an important role in nodule size of soybean–Bradyrhizobium symbiosis. However, it was possible that not only IAA and cytokinin but also some other substances secreted from S141 facilitate Bradyrhizobium to trigger bigger nodule formation, resulting in enhanced N2-fixation. Therefore, the ability of S141 with Bradyrhizobium co-inoculation to enhance soybean N2-fixation strategy could be further developed for supreme soybean inoculants.
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spelling pubmed-72846912020-06-15 Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation Sibponkrung, Surachat Kondo, Takahiko Tanaka, Kosei Tittabutr, Panlada Boonkerd, Nantakorn Yoshida, Ken-ichi Teaumroong, Neung Microorganisms Article The objective of this research was to evaluate the PGPR effect on nodulation and nitrogen-fixing efficiency of soybean (Glycine max (L.) Merr.) by co-inoculation with Bradyrhizobium diazoefficiens USDA110. Co-inoculation of Bacillus velezensis S141 with USDA110 into soybean resulted in enhanced nodulation and N2-fixing efficiency by producing larger nodules. To understand the role of S141 on soybean and USDA110 symbiosis, putative genes related to IAA biosynthesis were disrupted, suggesting that co-inoculation of USDA110 with S141ΔyhcX reduces the number of large size nodules. It was revealed that yhcX may play a major role in IAA biosynthesis in S141 as well as provide a major impact on soybean growth promotion. The disruption of genes related to cytokinin biosynthesis and co-inoculation of USDA110 with S141ΔIPI reduced the number of very large size nodules, and it appears that IPI might play an important role in nodule size of soybean–Bradyrhizobium symbiosis. However, it was possible that not only IAA and cytokinin but also some other substances secreted from S141 facilitate Bradyrhizobium to trigger bigger nodule formation, resulting in enhanced N2-fixation. Therefore, the ability of S141 with Bradyrhizobium co-inoculation to enhance soybean N2-fixation strategy could be further developed for supreme soybean inoculants. MDPI 2020-05-07 /pmc/articles/PMC7284691/ /pubmed/32392716 http://dx.doi.org/10.3390/microorganisms8050678 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sibponkrung, Surachat
Kondo, Takahiko
Tanaka, Kosei
Tittabutr, Panlada
Boonkerd, Nantakorn
Yoshida, Ken-ichi
Teaumroong, Neung
Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation
title Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation
title_full Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation
title_fullStr Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation
title_full_unstemmed Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation
title_short Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation
title_sort co-inoculation of bacillus velezensis strain s141 and bradyrhizobium strains promotes nodule growth and nitrogen fixation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284691/
https://www.ncbi.nlm.nih.gov/pubmed/32392716
http://dx.doi.org/10.3390/microorganisms8050678
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