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Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection

The human cytomegalovirus (HCMV) is a widespread pathogen and is associated with severe diseases in immunocompromised individuals. Moreover, HCMV infection is the most frequent cause of congenital malformation in developed countries. Although nucleoside analogs have been successfully employed agains...

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Autores principales: Biolatti, Matteo, Blangetti, Marco, D’Arrigo, Giulia, Spyrakis, Francesca, Cappello, Paola, Albano, Camilla, Ravanini, Paolo, Landolfo, Santo, De Andrea, Marco, Prandi, Cristina, Dell’Oste, Valentina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284764/
https://www.ncbi.nlm.nih.gov/pubmed/32397638
http://dx.doi.org/10.3390/microorganisms8050703
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author Biolatti, Matteo
Blangetti, Marco
D’Arrigo, Giulia
Spyrakis, Francesca
Cappello, Paola
Albano, Camilla
Ravanini, Paolo
Landolfo, Santo
De Andrea, Marco
Prandi, Cristina
Dell’Oste, Valentina
author_facet Biolatti, Matteo
Blangetti, Marco
D’Arrigo, Giulia
Spyrakis, Francesca
Cappello, Paola
Albano, Camilla
Ravanini, Paolo
Landolfo, Santo
De Andrea, Marco
Prandi, Cristina
Dell’Oste, Valentina
author_sort Biolatti, Matteo
collection PubMed
description The human cytomegalovirus (HCMV) is a widespread pathogen and is associated with severe diseases in immunocompromised individuals. Moreover, HCMV infection is the most frequent cause of congenital malformation in developed countries. Although nucleoside analogs have been successfully employed against HCMV, their use is hampered by the occurrence of serious side effects. There is thus an urgent clinical need for less toxic, but highly effective, antiviral drugs. Strigolactones (SLs) are a novel class of plant hormones with a multifaceted activity. While their role in plant-related fields has been extensively explored, their effects on human cells and their potential applications in medicine are far from being fully exploited. In particular, their antiviral activity has never been investigated. In the present study, a panel of SL analogs has been assessed for antiviral activity against HCMV. We demonstrate that TH-EGO and EDOT-EGO significantly inhibit HCMV replication in vitro, impairing late protein expression. Moreover, we show that the SL-dependent induction of apoptosis in HCMV-infected cells is a contributing mechanism to SL antiviral properties. Overall, our results indicate that SLs may be a promising alternative to nucleoside analogs for the treatment of HCMV infections.
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spelling pubmed-72847642020-06-15 Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection Biolatti, Matteo Blangetti, Marco D’Arrigo, Giulia Spyrakis, Francesca Cappello, Paola Albano, Camilla Ravanini, Paolo Landolfo, Santo De Andrea, Marco Prandi, Cristina Dell’Oste, Valentina Microorganisms Article The human cytomegalovirus (HCMV) is a widespread pathogen and is associated with severe diseases in immunocompromised individuals. Moreover, HCMV infection is the most frequent cause of congenital malformation in developed countries. Although nucleoside analogs have been successfully employed against HCMV, their use is hampered by the occurrence of serious side effects. There is thus an urgent clinical need for less toxic, but highly effective, antiviral drugs. Strigolactones (SLs) are a novel class of plant hormones with a multifaceted activity. While their role in plant-related fields has been extensively explored, their effects on human cells and their potential applications in medicine are far from being fully exploited. In particular, their antiviral activity has never been investigated. In the present study, a panel of SL analogs has been assessed for antiviral activity against HCMV. We demonstrate that TH-EGO and EDOT-EGO significantly inhibit HCMV replication in vitro, impairing late protein expression. Moreover, we show that the SL-dependent induction of apoptosis in HCMV-infected cells is a contributing mechanism to SL antiviral properties. Overall, our results indicate that SLs may be a promising alternative to nucleoside analogs for the treatment of HCMV infections. MDPI 2020-05-10 /pmc/articles/PMC7284764/ /pubmed/32397638 http://dx.doi.org/10.3390/microorganisms8050703 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Biolatti, Matteo
Blangetti, Marco
D’Arrigo, Giulia
Spyrakis, Francesca
Cappello, Paola
Albano, Camilla
Ravanini, Paolo
Landolfo, Santo
De Andrea, Marco
Prandi, Cristina
Dell’Oste, Valentina
Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection
title Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection
title_full Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection
title_fullStr Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection
title_full_unstemmed Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection
title_short Strigolactone Analogs Are Promising Antiviral Agents for the Treatment of Human Cytomegalovirus Infection
title_sort strigolactone analogs are promising antiviral agents for the treatment of human cytomegalovirus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284764/
https://www.ncbi.nlm.nih.gov/pubmed/32397638
http://dx.doi.org/10.3390/microorganisms8050703
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