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Cell Theranostics on Mesoporous Silicon Substrates
The adhesion, proliferation, and migration of cells over nanomaterials is regulated by a cascade of biochemical signals that originate at the interface of a cell with a substrate and propagate through the cytoplasm to the nucleus. The topography of the substrate plays a major role in this process. C...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284777/ https://www.ncbi.nlm.nih.gov/pubmed/32466284 http://dx.doi.org/10.3390/pharmaceutics12050481 |
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author | Coluccio, Maria Laura Onesto, Valentina Marinaro, Giovanni Dell’Apa, Mauro De Vitis, Stefania Imbrogno, Alessandra Tirinato, Luca Perozziello, Gerardo Di Fabrizio, Enzo Candeloro, Patrizio Malara, Natalia Gentile, Francesco |
author_facet | Coluccio, Maria Laura Onesto, Valentina Marinaro, Giovanni Dell’Apa, Mauro De Vitis, Stefania Imbrogno, Alessandra Tirinato, Luca Perozziello, Gerardo Di Fabrizio, Enzo Candeloro, Patrizio Malara, Natalia Gentile, Francesco |
author_sort | Coluccio, Maria Laura |
collection | PubMed |
description | The adhesion, proliferation, and migration of cells over nanomaterials is regulated by a cascade of biochemical signals that originate at the interface of a cell with a substrate and propagate through the cytoplasm to the nucleus. The topography of the substrate plays a major role in this process. Cell adhesion molecules (CAMs) have a characteristic size of some nanometers and a range of action of some tens of nanometers. Controlling details of a surface at the nanoscale—the same dimensional over which CAMs operate—offers ways to govern the behavior of cells and create organoids or tissues with heretofore unattainable precision. Here, using electrochemical procedures, we generated mesoporous silicon surfaces with different values of pore size (PS ≈ 11 nm and PS ≈ 21 nm), roughness (Ra ≈ 7 nm and Ra ≈ 13 nm), and fractal dimension (Df ≈ 2.48 and Df ≈ 2.15). Using electroless deposition, we deposited over these substrates thin layers of gold nanoparticles. Resulting devices feature (i) nanoscale details for the stimulation and control of cell assembly, (ii) arrays of pores for drug loading/release, (iii) layers of nanostructured gold for the enhancement of the electromagnetic signal in Raman spectroscopy (SERS). We then used these devices as cell culturing substrates. Upon loading with the anti-tumor drug PtCl (O,O′-acac)(DMSO) we examined the rate of adhesion and growth of breast cancer MCF-7 cells under the coincidental effects of surface geometry and drug release. Using confocal imaging and SERS spectroscopy we determined the relative importance of nano-topography and delivery of therapeutics on cell growth—and how an unbalance between these competing agents can accelerate the development of tumor cells. |
format | Online Article Text |
id | pubmed-7284777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72847772020-06-15 Cell Theranostics on Mesoporous Silicon Substrates Coluccio, Maria Laura Onesto, Valentina Marinaro, Giovanni Dell’Apa, Mauro De Vitis, Stefania Imbrogno, Alessandra Tirinato, Luca Perozziello, Gerardo Di Fabrizio, Enzo Candeloro, Patrizio Malara, Natalia Gentile, Francesco Pharmaceutics Article The adhesion, proliferation, and migration of cells over nanomaterials is regulated by a cascade of biochemical signals that originate at the interface of a cell with a substrate and propagate through the cytoplasm to the nucleus. The topography of the substrate plays a major role in this process. Cell adhesion molecules (CAMs) have a characteristic size of some nanometers and a range of action of some tens of nanometers. Controlling details of a surface at the nanoscale—the same dimensional over which CAMs operate—offers ways to govern the behavior of cells and create organoids or tissues with heretofore unattainable precision. Here, using electrochemical procedures, we generated mesoporous silicon surfaces with different values of pore size (PS ≈ 11 nm and PS ≈ 21 nm), roughness (Ra ≈ 7 nm and Ra ≈ 13 nm), and fractal dimension (Df ≈ 2.48 and Df ≈ 2.15). Using electroless deposition, we deposited over these substrates thin layers of gold nanoparticles. Resulting devices feature (i) nanoscale details for the stimulation and control of cell assembly, (ii) arrays of pores for drug loading/release, (iii) layers of nanostructured gold for the enhancement of the electromagnetic signal in Raman spectroscopy (SERS). We then used these devices as cell culturing substrates. Upon loading with the anti-tumor drug PtCl (O,O′-acac)(DMSO) we examined the rate of adhesion and growth of breast cancer MCF-7 cells under the coincidental effects of surface geometry and drug release. Using confocal imaging and SERS spectroscopy we determined the relative importance of nano-topography and delivery of therapeutics on cell growth—and how an unbalance between these competing agents can accelerate the development of tumor cells. MDPI 2020-05-25 /pmc/articles/PMC7284777/ /pubmed/32466284 http://dx.doi.org/10.3390/pharmaceutics12050481 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Coluccio, Maria Laura Onesto, Valentina Marinaro, Giovanni Dell’Apa, Mauro De Vitis, Stefania Imbrogno, Alessandra Tirinato, Luca Perozziello, Gerardo Di Fabrizio, Enzo Candeloro, Patrizio Malara, Natalia Gentile, Francesco Cell Theranostics on Mesoporous Silicon Substrates |
title | Cell Theranostics on Mesoporous Silicon Substrates |
title_full | Cell Theranostics on Mesoporous Silicon Substrates |
title_fullStr | Cell Theranostics on Mesoporous Silicon Substrates |
title_full_unstemmed | Cell Theranostics on Mesoporous Silicon Substrates |
title_short | Cell Theranostics on Mesoporous Silicon Substrates |
title_sort | cell theranostics on mesoporous silicon substrates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284777/ https://www.ncbi.nlm.nih.gov/pubmed/32466284 http://dx.doi.org/10.3390/pharmaceutics12050481 |
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