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Cell Theranostics on Mesoporous Silicon Substrates

The adhesion, proliferation, and migration of cells over nanomaterials is regulated by a cascade of biochemical signals that originate at the interface of a cell with a substrate and propagate through the cytoplasm to the nucleus. The topography of the substrate plays a major role in this process. C...

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Autores principales: Coluccio, Maria Laura, Onesto, Valentina, Marinaro, Giovanni, Dell’Apa, Mauro, De Vitis, Stefania, Imbrogno, Alessandra, Tirinato, Luca, Perozziello, Gerardo, Di Fabrizio, Enzo, Candeloro, Patrizio, Malara, Natalia, Gentile, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284777/
https://www.ncbi.nlm.nih.gov/pubmed/32466284
http://dx.doi.org/10.3390/pharmaceutics12050481
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author Coluccio, Maria Laura
Onesto, Valentina
Marinaro, Giovanni
Dell’Apa, Mauro
De Vitis, Stefania
Imbrogno, Alessandra
Tirinato, Luca
Perozziello, Gerardo
Di Fabrizio, Enzo
Candeloro, Patrizio
Malara, Natalia
Gentile, Francesco
author_facet Coluccio, Maria Laura
Onesto, Valentina
Marinaro, Giovanni
Dell’Apa, Mauro
De Vitis, Stefania
Imbrogno, Alessandra
Tirinato, Luca
Perozziello, Gerardo
Di Fabrizio, Enzo
Candeloro, Patrizio
Malara, Natalia
Gentile, Francesco
author_sort Coluccio, Maria Laura
collection PubMed
description The adhesion, proliferation, and migration of cells over nanomaterials is regulated by a cascade of biochemical signals that originate at the interface of a cell with a substrate and propagate through the cytoplasm to the nucleus. The topography of the substrate plays a major role in this process. Cell adhesion molecules (CAMs) have a characteristic size of some nanometers and a range of action of some tens of nanometers. Controlling details of a surface at the nanoscale—the same dimensional over which CAMs operate—offers ways to govern the behavior of cells and create organoids or tissues with heretofore unattainable precision. Here, using electrochemical procedures, we generated mesoporous silicon surfaces with different values of pore size (PS ≈ 11 nm and PS ≈ 21 nm), roughness (Ra ≈ 7 nm and Ra ≈ 13 nm), and fractal dimension (Df ≈ 2.48 and Df ≈ 2.15). Using electroless deposition, we deposited over these substrates thin layers of gold nanoparticles. Resulting devices feature (i) nanoscale details for the stimulation and control of cell assembly, (ii) arrays of pores for drug loading/release, (iii) layers of nanostructured gold for the enhancement of the electromagnetic signal in Raman spectroscopy (SERS). We then used these devices as cell culturing substrates. Upon loading with the anti-tumor drug PtCl (O,O′-acac)(DMSO) we examined the rate of adhesion and growth of breast cancer MCF-7 cells under the coincidental effects of surface geometry and drug release. Using confocal imaging and SERS spectroscopy we determined the relative importance of nano-topography and delivery of therapeutics on cell growth—and how an unbalance between these competing agents can accelerate the development of tumor cells.
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spelling pubmed-72847772020-06-15 Cell Theranostics on Mesoporous Silicon Substrates Coluccio, Maria Laura Onesto, Valentina Marinaro, Giovanni Dell’Apa, Mauro De Vitis, Stefania Imbrogno, Alessandra Tirinato, Luca Perozziello, Gerardo Di Fabrizio, Enzo Candeloro, Patrizio Malara, Natalia Gentile, Francesco Pharmaceutics Article The adhesion, proliferation, and migration of cells over nanomaterials is regulated by a cascade of biochemical signals that originate at the interface of a cell with a substrate and propagate through the cytoplasm to the nucleus. The topography of the substrate plays a major role in this process. Cell adhesion molecules (CAMs) have a characteristic size of some nanometers and a range of action of some tens of nanometers. Controlling details of a surface at the nanoscale—the same dimensional over which CAMs operate—offers ways to govern the behavior of cells and create organoids or tissues with heretofore unattainable precision. Here, using electrochemical procedures, we generated mesoporous silicon surfaces with different values of pore size (PS ≈ 11 nm and PS ≈ 21 nm), roughness (Ra ≈ 7 nm and Ra ≈ 13 nm), and fractal dimension (Df ≈ 2.48 and Df ≈ 2.15). Using electroless deposition, we deposited over these substrates thin layers of gold nanoparticles. Resulting devices feature (i) nanoscale details for the stimulation and control of cell assembly, (ii) arrays of pores for drug loading/release, (iii) layers of nanostructured gold for the enhancement of the electromagnetic signal in Raman spectroscopy (SERS). We then used these devices as cell culturing substrates. Upon loading with the anti-tumor drug PtCl (O,O′-acac)(DMSO) we examined the rate of adhesion and growth of breast cancer MCF-7 cells under the coincidental effects of surface geometry and drug release. Using confocal imaging and SERS spectroscopy we determined the relative importance of nano-topography and delivery of therapeutics on cell growth—and how an unbalance between these competing agents can accelerate the development of tumor cells. MDPI 2020-05-25 /pmc/articles/PMC7284777/ /pubmed/32466284 http://dx.doi.org/10.3390/pharmaceutics12050481 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Coluccio, Maria Laura
Onesto, Valentina
Marinaro, Giovanni
Dell’Apa, Mauro
De Vitis, Stefania
Imbrogno, Alessandra
Tirinato, Luca
Perozziello, Gerardo
Di Fabrizio, Enzo
Candeloro, Patrizio
Malara, Natalia
Gentile, Francesco
Cell Theranostics on Mesoporous Silicon Substrates
title Cell Theranostics on Mesoporous Silicon Substrates
title_full Cell Theranostics on Mesoporous Silicon Substrates
title_fullStr Cell Theranostics on Mesoporous Silicon Substrates
title_full_unstemmed Cell Theranostics on Mesoporous Silicon Substrates
title_short Cell Theranostics on Mesoporous Silicon Substrates
title_sort cell theranostics on mesoporous silicon substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284777/
https://www.ncbi.nlm.nih.gov/pubmed/32466284
http://dx.doi.org/10.3390/pharmaceutics12050481
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