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Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep

Chorioamnionitis can lead to inflammation and injury of the liver and gut, thereby predisposing patients to adverse outcomes such as necrotizing enterocolitis (NEC). In addition, intestinal bile acids (BAs) accumulation is causally linked to NEC development. Plant sterols are a promising interventio...

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Autores principales: Heymans, Cathelijne, Heij, Lara R., Lenaerts, Kaatje, den Dulk, Marcel, Hadfoune, Mhamed, van Heugten, Chantal, Spiller, Owen B., Beeton, Michael L., Stock, Sarah J., Jobe, Alan H., Payne, Matthew S., Kemp, Matthew W., Kramer, Boris W., Plat, Jogchum, van Gemert, Wim G., Wolfs, Tim G.A.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284867/
https://www.ncbi.nlm.nih.gov/pubmed/32380648
http://dx.doi.org/10.3390/nu12051312
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author Heymans, Cathelijne
Heij, Lara R.
Lenaerts, Kaatje
den Dulk, Marcel
Hadfoune, Mhamed
van Heugten, Chantal
Spiller, Owen B.
Beeton, Michael L.
Stock, Sarah J.
Jobe, Alan H.
Payne, Matthew S.
Kemp, Matthew W.
Kramer, Boris W.
Plat, Jogchum
van Gemert, Wim G.
Wolfs, Tim G.A.M.
author_facet Heymans, Cathelijne
Heij, Lara R.
Lenaerts, Kaatje
den Dulk, Marcel
Hadfoune, Mhamed
van Heugten, Chantal
Spiller, Owen B.
Beeton, Michael L.
Stock, Sarah J.
Jobe, Alan H.
Payne, Matthew S.
Kemp, Matthew W.
Kramer, Boris W.
Plat, Jogchum
van Gemert, Wim G.
Wolfs, Tim G.A.M.
author_sort Heymans, Cathelijne
collection PubMed
description Chorioamnionitis can lead to inflammation and injury of the liver and gut, thereby predisposing patients to adverse outcomes such as necrotizing enterocolitis (NEC). In addition, intestinal bile acids (BAs) accumulation is causally linked to NEC development. Plant sterols are a promising intervention to prevent NEC development, considering their anti-inflammatory properties in the liver. Therefore, we investigated whether an intra-amniotic (IA) Ureaplasma parvum (UP) infection affected the liver and enterohepatic circulation (EHC) and evaluated whether an IA administered plant sterol mixture dissolved in β-cyclodextrin exerted prophylactic effects. An ovine chorioamnionitis model was used in which liver inflammation and the EHC were assessed following IA UP exposure in the presence or absence of IA prophylactic plant sterols (a mixture of β-sitosterol and campesterol dissolved in β-cyclodextrin (carrier)) or carrier alone. IA UP exposure caused an inflammatory reaction in the liver, histologically seen as clustered and conflated hepatic erythropoiesis in the parenchyma, which was partially prevented by IA administration of sterol + β-cyclodextrin, or β-cyclodextrin alone. In addition, IA administration of β-cyclodextrin prior to UP caused changes in the expression of several hepatic BAs transporters, without causing alterations in other aspects of the EHC. Thereby, the addition of plant sterols to the carrier β-cyclodextrin did not have additional effects.
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spelling pubmed-72848672020-06-17 Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep Heymans, Cathelijne Heij, Lara R. Lenaerts, Kaatje den Dulk, Marcel Hadfoune, Mhamed van Heugten, Chantal Spiller, Owen B. Beeton, Michael L. Stock, Sarah J. Jobe, Alan H. Payne, Matthew S. Kemp, Matthew W. Kramer, Boris W. Plat, Jogchum van Gemert, Wim G. Wolfs, Tim G.A.M. Nutrients Article Chorioamnionitis can lead to inflammation and injury of the liver and gut, thereby predisposing patients to adverse outcomes such as necrotizing enterocolitis (NEC). In addition, intestinal bile acids (BAs) accumulation is causally linked to NEC development. Plant sterols are a promising intervention to prevent NEC development, considering their anti-inflammatory properties in the liver. Therefore, we investigated whether an intra-amniotic (IA) Ureaplasma parvum (UP) infection affected the liver and enterohepatic circulation (EHC) and evaluated whether an IA administered plant sterol mixture dissolved in β-cyclodextrin exerted prophylactic effects. An ovine chorioamnionitis model was used in which liver inflammation and the EHC were assessed following IA UP exposure in the presence or absence of IA prophylactic plant sterols (a mixture of β-sitosterol and campesterol dissolved in β-cyclodextrin (carrier)) or carrier alone. IA UP exposure caused an inflammatory reaction in the liver, histologically seen as clustered and conflated hepatic erythropoiesis in the parenchyma, which was partially prevented by IA administration of sterol + β-cyclodextrin, or β-cyclodextrin alone. In addition, IA administration of β-cyclodextrin prior to UP caused changes in the expression of several hepatic BAs transporters, without causing alterations in other aspects of the EHC. Thereby, the addition of plant sterols to the carrier β-cyclodextrin did not have additional effects. MDPI 2020-05-05 /pmc/articles/PMC7284867/ /pubmed/32380648 http://dx.doi.org/10.3390/nu12051312 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Heymans, Cathelijne
Heij, Lara R.
Lenaerts, Kaatje
den Dulk, Marcel
Hadfoune, Mhamed
van Heugten, Chantal
Spiller, Owen B.
Beeton, Michael L.
Stock, Sarah J.
Jobe, Alan H.
Payne, Matthew S.
Kemp, Matthew W.
Kramer, Boris W.
Plat, Jogchum
van Gemert, Wim G.
Wolfs, Tim G.A.M.
Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep
title Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep
title_full Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep
title_fullStr Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep
title_full_unstemmed Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep
title_short Prophylactic Intra-Uterine β-Cyclodextrin Administration during Intra-Uterine Ureaplasma parvum Infection Partly Prevents Liver Inflammation without Interfering with the Enterohepatic Circulation of the Fetal Sheep
title_sort prophylactic intra-uterine β-cyclodextrin administration during intra-uterine ureaplasma parvum infection partly prevents liver inflammation without interfering with the enterohepatic circulation of the fetal sheep
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284867/
https://www.ncbi.nlm.nih.gov/pubmed/32380648
http://dx.doi.org/10.3390/nu12051312
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