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Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control

Bayberry (Myrica rubra) is a commercial fruit in China. For the past seven years, twig blight disease has been attacking bayberry plantations in Shantou City, Guangdong Province, China, leading to destructive damage and financial loss. In this study, five fungal species associated with twig dieback...

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Autores principales: Li, Wenjun, Hu, Ming, Xue, Yang, Li, Zhijun, Zhang, Yanfei, Zheng, Daoxu, Lu, Guangtao, Wang, Junxia, Zhou, Jianuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284972/
https://www.ncbi.nlm.nih.gov/pubmed/32397322
http://dx.doi.org/10.3390/microorganisms8050689
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author Li, Wenjun
Hu, Ming
Xue, Yang
Li, Zhijun
Zhang, Yanfei
Zheng, Daoxu
Lu, Guangtao
Wang, Junxia
Zhou, Jianuan
author_facet Li, Wenjun
Hu, Ming
Xue, Yang
Li, Zhijun
Zhang, Yanfei
Zheng, Daoxu
Lu, Guangtao
Wang, Junxia
Zhou, Jianuan
author_sort Li, Wenjun
collection PubMed
description Bayberry (Myrica rubra) is a commercial fruit in China. For the past seven years, twig blight disease has been attacking bayberry plantations in Shantou City, Guangdong Province, China, leading to destructive damage and financial loss. In this study, five fungal species associated with twig dieback and stem blight were identified based on morphological characteristics combined with multilocus sequence analysis (MLSA) on the internal transcribed spacer (ITS) region, partial sequences of β-tubulin (tub2), translation elongation factor 1-α (tef1-α), large subunit ribosomal RNA (LSU) and small subunit ribosomal RNA (SSU) genes, which are Epicoccum sorghinum, Neofusicoccum parvum, Lasiodiplodia theobromae, Nigrospora oryzae and a Pestalotiopsis new species P. myricae. P. myricae is the chief pathogen in fields, based on its high isolation rate and fast disease progression after inoculation. To our knowledge, this is the first study reporting the above five fungi as the pathogens responsible for bayberry twig blight. Indoor screening of fungicides indicates that Prochloraz (copper salt) is the most promising fungicide for field application, followed by Pyraclostrobin, 15% Difenoconazole + 15% Propiconazole, Difenoconazole and Myclobutanil. Additionally, Bacillus velezensis strain 3–10 and zeamines from Dickeya zeae strain EC1 could be used as potential ecofriendly alternatives to control the disease.
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spelling pubmed-72849722020-06-17 Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control Li, Wenjun Hu, Ming Xue, Yang Li, Zhijun Zhang, Yanfei Zheng, Daoxu Lu, Guangtao Wang, Junxia Zhou, Jianuan Microorganisms Article Bayberry (Myrica rubra) is a commercial fruit in China. For the past seven years, twig blight disease has been attacking bayberry plantations in Shantou City, Guangdong Province, China, leading to destructive damage and financial loss. In this study, five fungal species associated with twig dieback and stem blight were identified based on morphological characteristics combined with multilocus sequence analysis (MLSA) on the internal transcribed spacer (ITS) region, partial sequences of β-tubulin (tub2), translation elongation factor 1-α (tef1-α), large subunit ribosomal RNA (LSU) and small subunit ribosomal RNA (SSU) genes, which are Epicoccum sorghinum, Neofusicoccum parvum, Lasiodiplodia theobromae, Nigrospora oryzae and a Pestalotiopsis new species P. myricae. P. myricae is the chief pathogen in fields, based on its high isolation rate and fast disease progression after inoculation. To our knowledge, this is the first study reporting the above five fungi as the pathogens responsible for bayberry twig blight. Indoor screening of fungicides indicates that Prochloraz (copper salt) is the most promising fungicide for field application, followed by Pyraclostrobin, 15% Difenoconazole + 15% Propiconazole, Difenoconazole and Myclobutanil. Additionally, Bacillus velezensis strain 3–10 and zeamines from Dickeya zeae strain EC1 could be used as potential ecofriendly alternatives to control the disease. MDPI 2020-05-08 /pmc/articles/PMC7284972/ /pubmed/32397322 http://dx.doi.org/10.3390/microorganisms8050689 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Wenjun
Hu, Ming
Xue, Yang
Li, Zhijun
Zhang, Yanfei
Zheng, Daoxu
Lu, Guangtao
Wang, Junxia
Zhou, Jianuan
Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control
title Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control
title_full Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control
title_fullStr Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control
title_full_unstemmed Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control
title_short Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control
title_sort five fungal pathogens are responsible for bayberry twig blight and fungicides were screened for disease control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284972/
https://www.ncbi.nlm.nih.gov/pubmed/32397322
http://dx.doi.org/10.3390/microorganisms8050689
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