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High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules

Poly(methyl-methacrylate) (PMMA) is a biocompatible and non-biodegradable polymer widely used as biomedical material. PMMA microcapsules with suitable dimension and porosity range are proposed to encapsulate live cells useful for tissue regeneration purposes. The aim of this work was to evaluate the...

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Autores principales: Pisani, Silvia, Dorati, Rossella, Genta, Ida, Chiesa, Enrica, Modena, Tiziana, Conti, Bice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284989/
https://www.ncbi.nlm.nih.gov/pubmed/32455714
http://dx.doi.org/10.3390/pharmaceutics12050469
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author Pisani, Silvia
Dorati, Rossella
Genta, Ida
Chiesa, Enrica
Modena, Tiziana
Conti, Bice
author_facet Pisani, Silvia
Dorati, Rossella
Genta, Ida
Chiesa, Enrica
Modena, Tiziana
Conti, Bice
author_sort Pisani, Silvia
collection PubMed
description Poly(methyl-methacrylate) (PMMA) is a biocompatible and non-biodegradable polymer widely used as biomedical material. PMMA microcapsules with suitable dimension and porosity range are proposed to encapsulate live cells useful for tissue regeneration purposes. The aim of this work was to evaluate the feasibility of producing cell-loaded PMMA microcapsules through “high efficiency vibrational technology” (HEVT). Preliminary studies were conducted to set up the process parameters for PMMA microcapsules production and human dermal fibroblast, used as cell model, were encapsulated in shell/core microcapsules. Microcapsules morphometric analysis through optical microscope and scanning electron microscopy highlighted that uniform microcapsules of 1.2 mm with circular surface pores were obtained by HEVT. Best process conditions used were as follows: frequency of 200 Hz, voltage of 750 V, flow rate of core solution of 10 mL/min, and flow rate of shell solution of 0.5 bar. Microcapsule membrane allowed permeation of molecules with low and medium molecular weight up to 5900 Da and prevented diffusion of high molecular weight molecules (11,000 Da). The yield of the process was about 50% and cell encapsulation efficiency was 27% on total amount. The cell survived and growth up to 72 h incubation in simulated physiologic medium was observed.
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spelling pubmed-72849892020-06-17 High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules Pisani, Silvia Dorati, Rossella Genta, Ida Chiesa, Enrica Modena, Tiziana Conti, Bice Pharmaceutics Article Poly(methyl-methacrylate) (PMMA) is a biocompatible and non-biodegradable polymer widely used as biomedical material. PMMA microcapsules with suitable dimension and porosity range are proposed to encapsulate live cells useful for tissue regeneration purposes. The aim of this work was to evaluate the feasibility of producing cell-loaded PMMA microcapsules through “high efficiency vibrational technology” (HEVT). Preliminary studies were conducted to set up the process parameters for PMMA microcapsules production and human dermal fibroblast, used as cell model, were encapsulated in shell/core microcapsules. Microcapsules morphometric analysis through optical microscope and scanning electron microscopy highlighted that uniform microcapsules of 1.2 mm with circular surface pores were obtained by HEVT. Best process conditions used were as follows: frequency of 200 Hz, voltage of 750 V, flow rate of core solution of 10 mL/min, and flow rate of shell solution of 0.5 bar. Microcapsule membrane allowed permeation of molecules with low and medium molecular weight up to 5900 Da and prevented diffusion of high molecular weight molecules (11,000 Da). The yield of the process was about 50% and cell encapsulation efficiency was 27% on total amount. The cell survived and growth up to 72 h incubation in simulated physiologic medium was observed. MDPI 2020-05-21 /pmc/articles/PMC7284989/ /pubmed/32455714 http://dx.doi.org/10.3390/pharmaceutics12050469 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pisani, Silvia
Dorati, Rossella
Genta, Ida
Chiesa, Enrica
Modena, Tiziana
Conti, Bice
High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules
title High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules
title_full High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules
title_fullStr High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules
title_full_unstemmed High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules
title_short High Efficiency Vibrational Technology (HEVT) for Cell Encapsulation in Polymeric Microcapsules
title_sort high efficiency vibrational technology (hevt) for cell encapsulation in polymeric microcapsules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284989/
https://www.ncbi.nlm.nih.gov/pubmed/32455714
http://dx.doi.org/10.3390/pharmaceutics12050469
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