Cargando…
3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes
We investigated the effect of 3-iodothyronamine (T1AM) on thermogenic substrates in brown adipocytes (BAs). BAs isolated from the stromal fraction of rat brown adipose tissue were exposed to an adipogenic medium containing insulin in the absence (M) or in the presence of 20 nM T1AM (M+T1AM) for 6 da...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285105/ https://www.ncbi.nlm.nih.gov/pubmed/32375297 http://dx.doi.org/10.3390/biology9050095 |
_version_ | 1783544622185709568 |
---|---|
author | Gencarelli, Manuela Laurino, Annunziatina Landucci, Elisa Buonvicino, Daniela Mazzantini, Costanza Chiellini, Grazia Raimondi, Laura |
author_facet | Gencarelli, Manuela Laurino, Annunziatina Landucci, Elisa Buonvicino, Daniela Mazzantini, Costanza Chiellini, Grazia Raimondi, Laura |
author_sort | Gencarelli, Manuela |
collection | PubMed |
description | We investigated the effect of 3-iodothyronamine (T1AM) on thermogenic substrates in brown adipocytes (BAs). BAs isolated from the stromal fraction of rat brown adipose tissue were exposed to an adipogenic medium containing insulin in the absence (M) or in the presence of 20 nM T1AM (M+T1AM) for 6 days. At the end of the treatment, the expression of p-PKA/PKA, p-AKT/AKT, p-AMPK/AMPK, p-CREB/CREB, p-P38/P38, type 1 and 3 beta adrenergic receptors (β1–β3AR), GLUT4, type 2 deiodinase (DIO2), and uncoupling protein 1 (UCP-1) were evaluated. The effects of cell conditioning with T1AM on fatty acid mobilization (basal and adrenergic-mediated), glucose uptake (basal and insulin-mediated), and ATP cell content were also analyzed in both cell populations. When compared to cells not exposed, M+T1AM cells showed increased p-PKA/PKA, p-AKT/AKT, p-CREB/CREB, p-P38/P38, and p-AMPK/AMPK, downregulation of DIO2 and β1AR, and upregulation of glycosylated β3AR, GLUT4, and adiponectin. At basal conditions, glycerol release was higher for M+T1AM cells than M cells, without any significant differences in basal glucose uptake. Notably, in M+T1AM cells, adrenergic agonists failed to activate PKA and lipolysis and to increase ATP level, but the glucose uptake in response to insulin exposure was more pronounced than in M cells. In conclusion, our results suggest that BAs conditioning with T1AM promote a catabolic condition promising to fight obesity and insulin resistance. |
format | Online Article Text |
id | pubmed-7285105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72851052020-06-18 3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes Gencarelli, Manuela Laurino, Annunziatina Landucci, Elisa Buonvicino, Daniela Mazzantini, Costanza Chiellini, Grazia Raimondi, Laura Biology (Basel) Article We investigated the effect of 3-iodothyronamine (T1AM) on thermogenic substrates in brown adipocytes (BAs). BAs isolated from the stromal fraction of rat brown adipose tissue were exposed to an adipogenic medium containing insulin in the absence (M) or in the presence of 20 nM T1AM (M+T1AM) for 6 days. At the end of the treatment, the expression of p-PKA/PKA, p-AKT/AKT, p-AMPK/AMPK, p-CREB/CREB, p-P38/P38, type 1 and 3 beta adrenergic receptors (β1–β3AR), GLUT4, type 2 deiodinase (DIO2), and uncoupling protein 1 (UCP-1) were evaluated. The effects of cell conditioning with T1AM on fatty acid mobilization (basal and adrenergic-mediated), glucose uptake (basal and insulin-mediated), and ATP cell content were also analyzed in both cell populations. When compared to cells not exposed, M+T1AM cells showed increased p-PKA/PKA, p-AKT/AKT, p-CREB/CREB, p-P38/P38, and p-AMPK/AMPK, downregulation of DIO2 and β1AR, and upregulation of glycosylated β3AR, GLUT4, and adiponectin. At basal conditions, glycerol release was higher for M+T1AM cells than M cells, without any significant differences in basal glucose uptake. Notably, in M+T1AM cells, adrenergic agonists failed to activate PKA and lipolysis and to increase ATP level, but the glucose uptake in response to insulin exposure was more pronounced than in M cells. In conclusion, our results suggest that BAs conditioning with T1AM promote a catabolic condition promising to fight obesity and insulin resistance. MDPI 2020-05-04 /pmc/articles/PMC7285105/ /pubmed/32375297 http://dx.doi.org/10.3390/biology9050095 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gencarelli, Manuela Laurino, Annunziatina Landucci, Elisa Buonvicino, Daniela Mazzantini, Costanza Chiellini, Grazia Raimondi, Laura 3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes |
title | 3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes |
title_full | 3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes |
title_fullStr | 3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes |
title_full_unstemmed | 3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes |
title_short | 3-Iodothyronamine Affects Thermogenic Substrates’ Mobilization in Brown Adipocytes |
title_sort | 3-iodothyronamine affects thermogenic substrates’ mobilization in brown adipocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285105/ https://www.ncbi.nlm.nih.gov/pubmed/32375297 http://dx.doi.org/10.3390/biology9050095 |
work_keys_str_mv | AT gencarellimanuela 3iodothyronamineaffectsthermogenicsubstratesmobilizationinbrownadipocytes AT laurinoannunziatina 3iodothyronamineaffectsthermogenicsubstratesmobilizationinbrownadipocytes AT landuccielisa 3iodothyronamineaffectsthermogenicsubstratesmobilizationinbrownadipocytes AT buonvicinodaniela 3iodothyronamineaffectsthermogenicsubstratesmobilizationinbrownadipocytes AT mazzantinicostanza 3iodothyronamineaffectsthermogenicsubstratesmobilizationinbrownadipocytes AT chiellinigrazia 3iodothyronamineaffectsthermogenicsubstratesmobilizationinbrownadipocytes AT raimondilaura 3iodothyronamineaffectsthermogenicsubstratesmobilizationinbrownadipocytes |