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Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile

In addition to the industrial and biomedical applications of lithium, information on the tolerance of microorganisms to high Li concentrations in natural biological systems is limited. Strain LCHXa is a novel free-living Gram-positive, non-motile bacterium strain isolated from water samples taken at...

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Autores principales: Salazar-Ardiles, Camila, Caimanque, Tamara, Galetović, Alexandra, Vilo, Claudia, Araya, Jorge E., Flores, Nataly, Gómez-Silva, Benito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285145/
https://www.ncbi.nlm.nih.gov/pubmed/32380652
http://dx.doi.org/10.3390/microorganisms8050668
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author Salazar-Ardiles, Camila
Caimanque, Tamara
Galetović, Alexandra
Vilo, Claudia
Araya, Jorge E.
Flores, Nataly
Gómez-Silva, Benito
author_facet Salazar-Ardiles, Camila
Caimanque, Tamara
Galetović, Alexandra
Vilo, Claudia
Araya, Jorge E.
Flores, Nataly
Gómez-Silva, Benito
author_sort Salazar-Ardiles, Camila
collection PubMed
description In addition to the industrial and biomedical applications of lithium, information on the tolerance of microorganisms to high Li concentrations in natural biological systems is limited. Strain LCHXa is a novel free-living Gram-positive, non-motile bacterium strain isolated from water samples taken at Laguna Chaxa, a non-industrial water body with the highest soluble Li content (33 mM LiCl) within the Salar de Atacama basin in northern Chile. Enrichment was conducted in Luria-Bertani (LB) medium supplemented with 1 M LiCl. Strain LCHXa was a Novobiocin-resistant and coagulase negative Staphylococcus. Phylogenetically, strain LCHXa belongs to the species Staphylococcus sciuri. Strain LCHXa grew optimally in LB medium at pH 6–8 and 37 °C, and it was able to sustain growth at molar Li concentrations at 2 M LiCl, with a decrease in the specific growth rate of 85%. Osmoregulation in strain LCHXa partially involves glycine betaine and glycerol as compatible solutes.
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spelling pubmed-72851452020-06-18 Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile Salazar-Ardiles, Camila Caimanque, Tamara Galetović, Alexandra Vilo, Claudia Araya, Jorge E. Flores, Nataly Gómez-Silva, Benito Microorganisms Article In addition to the industrial and biomedical applications of lithium, information on the tolerance of microorganisms to high Li concentrations in natural biological systems is limited. Strain LCHXa is a novel free-living Gram-positive, non-motile bacterium strain isolated from water samples taken at Laguna Chaxa, a non-industrial water body with the highest soluble Li content (33 mM LiCl) within the Salar de Atacama basin in northern Chile. Enrichment was conducted in Luria-Bertani (LB) medium supplemented with 1 M LiCl. Strain LCHXa was a Novobiocin-resistant and coagulase negative Staphylococcus. Phylogenetically, strain LCHXa belongs to the species Staphylococcus sciuri. Strain LCHXa grew optimally in LB medium at pH 6–8 and 37 °C, and it was able to sustain growth at molar Li concentrations at 2 M LiCl, with a decrease in the specific growth rate of 85%. Osmoregulation in strain LCHXa partially involves glycine betaine and glycerol as compatible solutes. MDPI 2020-05-05 /pmc/articles/PMC7285145/ /pubmed/32380652 http://dx.doi.org/10.3390/microorganisms8050668 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salazar-Ardiles, Camila
Caimanque, Tamara
Galetović, Alexandra
Vilo, Claudia
Araya, Jorge E.
Flores, Nataly
Gómez-Silva, Benito
Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile
title Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile
title_full Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile
title_fullStr Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile
title_full_unstemmed Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile
title_short Staphylococcus sciuri Strain LCHXa is a Free-Living Lithium-Tolerant Bacterium Isolated from Salar de Atacama, Chile
title_sort staphylococcus sciuri strain lchxa is a free-living lithium-tolerant bacterium isolated from salar de atacama, chile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285145/
https://www.ncbi.nlm.nih.gov/pubmed/32380652
http://dx.doi.org/10.3390/microorganisms8050668
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