Cargando…

Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs

Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model o...

Descripción completa

Detalles Bibliográficos
Autores principales: Korzhikov-Vlakh, Viktor, Katernuk, Iuliia, Pilipenko, Iuliia, Lavrentieva, Antonina, Guryanov, Ivan, Sharoyko, Vladimir, Manshina, Alina A., Tennikova, Tatiana B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285230/
https://www.ncbi.nlm.nih.gov/pubmed/32397208
http://dx.doi.org/10.3390/polym12051077
_version_ 1783544651832098816
author Korzhikov-Vlakh, Viktor
Katernuk, Iuliia
Pilipenko, Iuliia
Lavrentieva, Antonina
Guryanov, Ivan
Sharoyko, Vladimir
Manshina, Alina A.
Tennikova, Tatiana B.
author_facet Korzhikov-Vlakh, Viktor
Katernuk, Iuliia
Pilipenko, Iuliia
Lavrentieva, Antonina
Guryanov, Ivan
Sharoyko, Vladimir
Manshina, Alina A.
Tennikova, Tatiana B.
author_sort Korzhikov-Vlakh, Viktor
collection PubMed
description Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model oligonucleotide, siRNA, and pDNA—were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated.
format Online
Article
Text
id pubmed-7285230
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72852302020-06-18 Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs Korzhikov-Vlakh, Viktor Katernuk, Iuliia Pilipenko, Iuliia Lavrentieva, Antonina Guryanov, Ivan Sharoyko, Vladimir Manshina, Alina A. Tennikova, Tatiana B. Polymers (Basel) Article Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model oligonucleotide, siRNA, and pDNA—were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated. MDPI 2020-05-08 /pmc/articles/PMC7285230/ /pubmed/32397208 http://dx.doi.org/10.3390/polym12051077 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Korzhikov-Vlakh, Viktor
Katernuk, Iuliia
Pilipenko, Iuliia
Lavrentieva, Antonina
Guryanov, Ivan
Sharoyko, Vladimir
Manshina, Alina A.
Tennikova, Tatiana B.
Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs
title Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs
title_full Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs
title_fullStr Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs
title_full_unstemmed Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs
title_short Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs
title_sort photosensitive poly-l-lysine/heparin interpolyelectrolyte complexes for delivery of genetic drugs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285230/
https://www.ncbi.nlm.nih.gov/pubmed/32397208
http://dx.doi.org/10.3390/polym12051077
work_keys_str_mv AT korzhikovvlakhviktor photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs
AT katernukiuliia photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs
AT pilipenkoiuliia photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs
AT lavrentievaantonina photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs
AT guryanovivan photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs
AT sharoykovladimir photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs
AT manshinaalinaa photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs
AT tennikovatatianab photosensitivepolyllysineheparininterpolyelectrolytecomplexesfordeliveryofgeneticdrugs