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Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs
Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285230/ https://www.ncbi.nlm.nih.gov/pubmed/32397208 http://dx.doi.org/10.3390/polym12051077 |
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author | Korzhikov-Vlakh, Viktor Katernuk, Iuliia Pilipenko, Iuliia Lavrentieva, Antonina Guryanov, Ivan Sharoyko, Vladimir Manshina, Alina A. Tennikova, Tatiana B. |
author_facet | Korzhikov-Vlakh, Viktor Katernuk, Iuliia Pilipenko, Iuliia Lavrentieva, Antonina Guryanov, Ivan Sharoyko, Vladimir Manshina, Alina A. Tennikova, Tatiana B. |
author_sort | Korzhikov-Vlakh, Viktor |
collection | PubMed |
description | Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model oligonucleotide, siRNA, and pDNA—were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated. |
format | Online Article Text |
id | pubmed-7285230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72852302020-06-18 Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs Korzhikov-Vlakh, Viktor Katernuk, Iuliia Pilipenko, Iuliia Lavrentieva, Antonina Guryanov, Ivan Sharoyko, Vladimir Manshina, Alina A. Tennikova, Tatiana B. Polymers (Basel) Article Photo-triggered release of biopharmaceutical drugs inside the cells is a challenging direction of modern science, which requires obtaining new polymeric systems. The interpolyelectrolyte complexes (IPECs) of poly-l-lysine with heparin capable of encapsulation of genetic constructions—such as model oligonucleotide, siRNA, and pDNA—were obtained. Poly-l-lysine to heparin ratios were optimized to provide the appropriate release kinetics of genetic material from the polyplex. In order to impart the obtained IPEC with photosensitive properties, the linker was synthesized as based on 4-brommethyl-3-nitrobenzoic acid. The conditions and kinetics of photosensitive linker destruction were carefully studied. The colloid particles of IPEC were modified with Cy3 probe and their cellular internalization was investigated by flow cytometry method. The efficacy of photosensitive IPECs as siRNA and pDNA delivery system was evaluated. MDPI 2020-05-08 /pmc/articles/PMC7285230/ /pubmed/32397208 http://dx.doi.org/10.3390/polym12051077 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Korzhikov-Vlakh, Viktor Katernuk, Iuliia Pilipenko, Iuliia Lavrentieva, Antonina Guryanov, Ivan Sharoyko, Vladimir Manshina, Alina A. Tennikova, Tatiana B. Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs |
title | Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs |
title_full | Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs |
title_fullStr | Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs |
title_full_unstemmed | Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs |
title_short | Photosensitive Poly-l-lysine/Heparin Interpolyelectrolyte Complexes for Delivery of Genetic Drugs |
title_sort | photosensitive poly-l-lysine/heparin interpolyelectrolyte complexes for delivery of genetic drugs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285230/ https://www.ncbi.nlm.nih.gov/pubmed/32397208 http://dx.doi.org/10.3390/polym12051077 |
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