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Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery
The diversity of actinomycetes associated with the marine sponge Coscinoderma mathewsi collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as novel species of t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285244/ https://www.ncbi.nlm.nih.gov/pubmed/32456212 http://dx.doi.org/10.3390/microorganisms8050783 |
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author | Shamikh, Yara I. El Shamy, Aliaa A. Gaber, Yasser Abdelmohsen, Usama Ramadan Madkour, Hashem A. Horn, Hannes Hassan, Hossam M. Elmaidomy, Abeer H. Alkhalifah, Dalal Hussien M. Hozzein, Wael N. |
author_facet | Shamikh, Yara I. El Shamy, Aliaa A. Gaber, Yasser Abdelmohsen, Usama Ramadan Madkour, Hashem A. Horn, Hannes Hassan, Hossam M. Elmaidomy, Abeer H. Alkhalifah, Dalal Hussien M. Hozzein, Wael N. |
author_sort | Shamikh, Yara I. |
collection | PubMed |
description | The diversity of actinomycetes associated with the marine sponge Coscinoderma mathewsi collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as novel species of the genera Micromonospora, Nocardia, and Gordonia. Genome sequencing of actinomycete strains has revealed many silent biosynthetic gene clusters and has shown their exceptional capacity for the production of secondary metabolites, not observed under classical cultivation conditions. Therefore, the effect of mycolic-acid-containing bacteria or mycolic acid on the biosynthesis of cryptic natural products was investigated. Sponge-derived actinomycete Micromonospora sp. UA17 was co-cultured using liquid fermentation with two mycolic acid-containing actinomycetes (Gordonia sp. UA19 and Nocardia sp. UA 23), or supplemented with pure mycolic acid. LC-HRESIMS data were analyzed to compare natural production across all crude extracts. Micromonospora sp. UA17 was rich with isotetracenone, indolocarbazole, and anthracycline analogs. Some co-culture extracts showed metabolites such as a chlorocardicin, neocopiamycin A, and chicamycin B that were not found in the respective monocultures, suggesting a mycolic acid effect on the induction of cryptic natural product biosynthetic pathways. The antibacterial, antifungal, and antiparasitic activities for the different cultures extracts were also tested. |
format | Online Article Text |
id | pubmed-7285244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72852442020-06-18 Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery Shamikh, Yara I. El Shamy, Aliaa A. Gaber, Yasser Abdelmohsen, Usama Ramadan Madkour, Hashem A. Horn, Hannes Hassan, Hossam M. Elmaidomy, Abeer H. Alkhalifah, Dalal Hussien M. Hozzein, Wael N. Microorganisms Article The diversity of actinomycetes associated with the marine sponge Coscinoderma mathewsi collected from Hurghada (Egypt) was studied. Twenty-three actinomycetes were separated and identified based on the 16S rDNA gene sequence analysis. Out of them, three isolates were classified as novel species of the genera Micromonospora, Nocardia, and Gordonia. Genome sequencing of actinomycete strains has revealed many silent biosynthetic gene clusters and has shown their exceptional capacity for the production of secondary metabolites, not observed under classical cultivation conditions. Therefore, the effect of mycolic-acid-containing bacteria or mycolic acid on the biosynthesis of cryptic natural products was investigated. Sponge-derived actinomycete Micromonospora sp. UA17 was co-cultured using liquid fermentation with two mycolic acid-containing actinomycetes (Gordonia sp. UA19 and Nocardia sp. UA 23), or supplemented with pure mycolic acid. LC-HRESIMS data were analyzed to compare natural production across all crude extracts. Micromonospora sp. UA17 was rich with isotetracenone, indolocarbazole, and anthracycline analogs. Some co-culture extracts showed metabolites such as a chlorocardicin, neocopiamycin A, and chicamycin B that were not found in the respective monocultures, suggesting a mycolic acid effect on the induction of cryptic natural product biosynthetic pathways. The antibacterial, antifungal, and antiparasitic activities for the different cultures extracts were also tested. MDPI 2020-05-23 /pmc/articles/PMC7285244/ /pubmed/32456212 http://dx.doi.org/10.3390/microorganisms8050783 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shamikh, Yara I. El Shamy, Aliaa A. Gaber, Yasser Abdelmohsen, Usama Ramadan Madkour, Hashem A. Horn, Hannes Hassan, Hossam M. Elmaidomy, Abeer H. Alkhalifah, Dalal Hussien M. Hozzein, Wael N. Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
title | Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
title_full | Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
title_fullStr | Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
title_full_unstemmed | Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
title_short | Actinomycetes from the Red Sea Sponge Coscinoderma mathewsi: Isolation, Diversity, and Potential for Bioactive Compounds Discovery |
title_sort | actinomycetes from the red sea sponge coscinoderma mathewsi: isolation, diversity, and potential for bioactive compounds discovery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285244/ https://www.ncbi.nlm.nih.gov/pubmed/32456212 http://dx.doi.org/10.3390/microorganisms8050783 |
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