Cargando…

Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial

BACKGROUND: Indians have a poor protein intake in terms of quantity as well as quality because of their predominantly cereal-based diet. However, there is limited information on circulatory amino acid levels in healthy Indians. Herein, we evaluated the acute effect of a protein supplement on the pla...

Descripción completa

Detalles Bibliográficos
Autores principales: Kurien, Ammu, Sharma, Nidhi, Marathe, Madhavi, Joshi, Nandan, Selvam, Sumithra, Devi, Sarita, Sambashivaiah, Sucharita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285395/
https://www.ncbi.nlm.nih.gov/pubmed/32566281
http://dx.doi.org/10.1155/2020/8946820
_version_ 1783544689018798080
author Kurien, Ammu
Sharma, Nidhi
Marathe, Madhavi
Joshi, Nandan
Selvam, Sumithra
Devi, Sarita
Sambashivaiah, Sucharita
author_facet Kurien, Ammu
Sharma, Nidhi
Marathe, Madhavi
Joshi, Nandan
Selvam, Sumithra
Devi, Sarita
Sambashivaiah, Sucharita
author_sort Kurien, Ammu
collection PubMed
description BACKGROUND: Indians have a poor protein intake in terms of quantity as well as quality because of their predominantly cereal-based diet. However, there is limited information on circulatory amino acid levels in healthy Indians. Herein, we evaluated the acute effect of a protein supplement on the plasma levels of essential amino acids (EAAs) in healthy Indian adults, using targeted EAA analysis. METHODS: In this double-blind, randomized, crossover study, 20 healthy Indian adults were randomized to receive the test protein supplement (treatment arm, n = 10) or placebo (control arm, n = 10) with milk, after overnight fasting. After 7 days, the participants returned for the crossover treatment. Blood samples were collected at baseline and at 60 and 120 min after protein/placebo consumption. Plasma EAA levels were estimated using liquid chromatography-tandem mass spectrometry. Repeated measures ANOVA was performed to assess the effect of treatment on EAA levels. P values < 0.05 were considered statistically significant. RESULTS: At baseline, mean plasma levels did not differ significantly between the two arms for any of the EAAs. In the treatment arm, the mean levels of all EAAs increased significantly from baseline to 60 min (P < 0.01), with no significant change from 60 to 120 min. There was no significant change in amino acid levels in the control arm. The magnitude as well as percentage of increase from baseline to 60 min was significantly greater in the treatment arm than in the control arm for all EAAs. CONCLUSION: Compared to placebo, protein supplement increased circulatory amino acid levels in healthy Indians. The observed increase in EAA levels and its role in conjunction with exercise in both healthy and diseased states need to be further evaluated. This is the first dataset exploring targeted EAA profiles and the effect of a protein supplement among healthy Indians. The clinical trial is registered with CTRI/2018/12/016777.
format Online
Article
Text
id pubmed-7285395
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-72853952020-06-19 Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial Kurien, Ammu Sharma, Nidhi Marathe, Madhavi Joshi, Nandan Selvam, Sumithra Devi, Sarita Sambashivaiah, Sucharita J Nutr Metab Research Article BACKGROUND: Indians have a poor protein intake in terms of quantity as well as quality because of their predominantly cereal-based diet. However, there is limited information on circulatory amino acid levels in healthy Indians. Herein, we evaluated the acute effect of a protein supplement on the plasma levels of essential amino acids (EAAs) in healthy Indian adults, using targeted EAA analysis. METHODS: In this double-blind, randomized, crossover study, 20 healthy Indian adults were randomized to receive the test protein supplement (treatment arm, n = 10) or placebo (control arm, n = 10) with milk, after overnight fasting. After 7 days, the participants returned for the crossover treatment. Blood samples were collected at baseline and at 60 and 120 min after protein/placebo consumption. Plasma EAA levels were estimated using liquid chromatography-tandem mass spectrometry. Repeated measures ANOVA was performed to assess the effect of treatment on EAA levels. P values < 0.05 were considered statistically significant. RESULTS: At baseline, mean plasma levels did not differ significantly between the two arms for any of the EAAs. In the treatment arm, the mean levels of all EAAs increased significantly from baseline to 60 min (P < 0.01), with no significant change from 60 to 120 min. There was no significant change in amino acid levels in the control arm. The magnitude as well as percentage of increase from baseline to 60 min was significantly greater in the treatment arm than in the control arm for all EAAs. CONCLUSION: Compared to placebo, protein supplement increased circulatory amino acid levels in healthy Indians. The observed increase in EAA levels and its role in conjunction with exercise in both healthy and diseased states need to be further evaluated. This is the first dataset exploring targeted EAA profiles and the effect of a protein supplement among healthy Indians. The clinical trial is registered with CTRI/2018/12/016777. Hindawi 2020-05-27 /pmc/articles/PMC7285395/ /pubmed/32566281 http://dx.doi.org/10.1155/2020/8946820 Text en Copyright © 2020 Ammu Kurien et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kurien, Ammu
Sharma, Nidhi
Marathe, Madhavi
Joshi, Nandan
Selvam, Sumithra
Devi, Sarita
Sambashivaiah, Sucharita
Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial
title Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial
title_full Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial
title_fullStr Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial
title_full_unstemmed Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial
title_short Acute Effect of a Protein Supplement on Targeted Plasma Amino Acid Profile among Healthy Asian Indians: A Randomized Controlled Trial
title_sort acute effect of a protein supplement on targeted plasma amino acid profile among healthy asian indians: a randomized controlled trial
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285395/
https://www.ncbi.nlm.nih.gov/pubmed/32566281
http://dx.doi.org/10.1155/2020/8946820
work_keys_str_mv AT kurienammu acuteeffectofaproteinsupplementontargetedplasmaaminoacidprofileamonghealthyasianindiansarandomizedcontrolledtrial
AT sharmanidhi acuteeffectofaproteinsupplementontargetedplasmaaminoacidprofileamonghealthyasianindiansarandomizedcontrolledtrial
AT marathemadhavi acuteeffectofaproteinsupplementontargetedplasmaaminoacidprofileamonghealthyasianindiansarandomizedcontrolledtrial
AT joshinandan acuteeffectofaproteinsupplementontargetedplasmaaminoacidprofileamonghealthyasianindiansarandomizedcontrolledtrial
AT selvamsumithra acuteeffectofaproteinsupplementontargetedplasmaaminoacidprofileamonghealthyasianindiansarandomizedcontrolledtrial
AT devisarita acuteeffectofaproteinsupplementontargetedplasmaaminoacidprofileamonghealthyasianindiansarandomizedcontrolledtrial
AT sambashivaiahsucharita acuteeffectofaproteinsupplementontargetedplasmaaminoacidprofileamonghealthyasianindiansarandomizedcontrolledtrial