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Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico

Snake venom may vary in composition and toxicity across the geographic distribution of a species. In the case of the three species of the Neotropical rattlesnakes Crotalus simus, C. culminatus and C. tzabcan recent research has revealed that their venoms can contain a neurotoxic component (crotoxin...

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Autores principales: Neri-Castro, Edgar, Hernández-Dávila, Arely, Olvera-Rodríguez, Alejandro, Cardoso-Torres, Héctor, Bénard-Valle, Melisa, Bastiaans, Elizabeth, López-Gutierrez, Oswaldo, Alagón, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286086/
https://www.ncbi.nlm.nih.gov/pubmed/32550564
http://dx.doi.org/10.1016/j.toxcx.2019.100007
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author Neri-Castro, Edgar
Hernández-Dávila, Arely
Olvera-Rodríguez, Alejandro
Cardoso-Torres, Héctor
Bénard-Valle, Melisa
Bastiaans, Elizabeth
López-Gutierrez, Oswaldo
Alagón, Alejandro
author_facet Neri-Castro, Edgar
Hernández-Dávila, Arely
Olvera-Rodríguez, Alejandro
Cardoso-Torres, Héctor
Bénard-Valle, Melisa
Bastiaans, Elizabeth
López-Gutierrez, Oswaldo
Alagón, Alejandro
author_sort Neri-Castro, Edgar
collection PubMed
description Snake venom may vary in composition and toxicity across the geographic distribution of a species. In the case of the three species of the Neotropical rattlesnakes Crotalus simus, C. culminatus and C. tzabcan recent research has revealed that their venoms can contain a neurotoxic component (crotoxin homologs), but is not always the case. In the present work, we detected and quantified crotoxin homologs in venom samples from three species distributed across Mexico, to describe variation at the individual and subspecific levels, using slot blot and ELISA immunoassays. We found that all C. simus individuals analyzed had substantial percentages of crotoxin homologs in their venoms (7.6–44.3%). In contrast, C. culminatus lacked them completely and six of ten individuals of the species C. tzabcan had low percentages (3.0–7.7%). We also found a direct relationship between the lethality of a venom and the percentage of crotoxin homologs it contained, indicating that the quantity of this component influences venom lethality in the rattlesnake C. simus.
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spelling pubmed-72860862020-06-16 Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico Neri-Castro, Edgar Hernández-Dávila, Arely Olvera-Rodríguez, Alejandro Cardoso-Torres, Héctor Bénard-Valle, Melisa Bastiaans, Elizabeth López-Gutierrez, Oswaldo Alagón, Alejandro Toxicon X Paper Snake venom may vary in composition and toxicity across the geographic distribution of a species. In the case of the three species of the Neotropical rattlesnakes Crotalus simus, C. culminatus and C. tzabcan recent research has revealed that their venoms can contain a neurotoxic component (crotoxin homologs), but is not always the case. In the present work, we detected and quantified crotoxin homologs in venom samples from three species distributed across Mexico, to describe variation at the individual and subspecific levels, using slot blot and ELISA immunoassays. We found that all C. simus individuals analyzed had substantial percentages of crotoxin homologs in their venoms (7.6–44.3%). In contrast, C. culminatus lacked them completely and six of ten individuals of the species C. tzabcan had low percentages (3.0–7.7%). We also found a direct relationship between the lethality of a venom and the percentage of crotoxin homologs it contained, indicating that the quantity of this component influences venom lethality in the rattlesnake C. simus. Elsevier 2019-02-05 /pmc/articles/PMC7286086/ /pubmed/32550564 http://dx.doi.org/10.1016/j.toxcx.2019.100007 Text en © 2019 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Paper
Neri-Castro, Edgar
Hernández-Dávila, Arely
Olvera-Rodríguez, Alejandro
Cardoso-Torres, Héctor
Bénard-Valle, Melisa
Bastiaans, Elizabeth
López-Gutierrez, Oswaldo
Alagón, Alejandro
Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico
title Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico
title_full Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico
title_fullStr Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico
title_full_unstemmed Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico
title_short Detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes Crotalus simus, C. culminatus and C. tzabcan from Mexico
title_sort detection and quantification of a β-neurotoxin (crotoxin homologs) in the venom of the rattlesnakes crotalus simus, c. culminatus and c. tzabcan from mexico
topic Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286086/
https://www.ncbi.nlm.nih.gov/pubmed/32550564
http://dx.doi.org/10.1016/j.toxcx.2019.100007
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