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Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event

Thirty-two 14-month old steers died during a period of four days (19–23 June 2017) after drinking from Junipers Reservoir (southeastern Oregon, USA) during a cyanobacterial bloom. Clinical and histopathological findings were consistent with acute liver disease, and microcystin-LR was present at 3000...

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Autores principales: Dreher, Theo W., Collart, Lindsay P., Mueller, Ryan S., Halsey, Kimberly H., Bildfell, Robert J., Schreder, Peter, Sobhakumari, Arya, Ferry, Rodney
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286090/
https://www.ncbi.nlm.nih.gov/pubmed/32831346
http://dx.doi.org/10.1016/j.toxcx.2018.100003
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author Dreher, Theo W.
Collart, Lindsay P.
Mueller, Ryan S.
Halsey, Kimberly H.
Bildfell, Robert J.
Schreder, Peter
Sobhakumari, Arya
Ferry, Rodney
author_facet Dreher, Theo W.
Collart, Lindsay P.
Mueller, Ryan S.
Halsey, Kimberly H.
Bildfell, Robert J.
Schreder, Peter
Sobhakumari, Arya
Ferry, Rodney
author_sort Dreher, Theo W.
collection PubMed
description Thirty-two 14-month old steers died during a period of four days (19–23 June 2017) after drinking from Junipers Reservoir (southeastern Oregon, USA) during a cyanobacterial bloom. Clinical and histopathological findings were consistent with acute liver disease, and microcystin-LR was present at 3000 μg/L in a reservoir water sample and at 7100 μg/L in the rumen contents of one of the mortalities. Serum biochemistry and histological examination indicated severe liver damage consistent with microcystin toxicosis. Microscopic observation of reservoir water samples, limited to frozen or poorly stored and partially degraded samples, indicated the presence of abundant Anabaena/Dolichospermum, but the presence of other toxic cyanobacteria such as Microcystis could not be excluded. Metagenomic analysis showed the presence in these samples of a single cyanobacterium whose cpcBA, rpoB and rbcL genes indicated membership in the Anabaena/Dolichospermum genus. The sequence of a complete mcy gene cluster with homology to previously identified Anabaena mcy genes was recovered. These results emphasize the capacity for Anabaena/Dolichospermum blooms to produce lethal levels of microcystin, posing a danger to public health and livestock. Further, our findings indicate that such occurrences can occur outside the far-northern latitudes in which microcystin-producing Anabaena have typically been found.
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spelling pubmed-72860902020-06-16 Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event Dreher, Theo W. Collart, Lindsay P. Mueller, Ryan S. Halsey, Kimberly H. Bildfell, Robert J. Schreder, Peter Sobhakumari, Arya Ferry, Rodney Toxicon X Paper Thirty-two 14-month old steers died during a period of four days (19–23 June 2017) after drinking from Junipers Reservoir (southeastern Oregon, USA) during a cyanobacterial bloom. Clinical and histopathological findings were consistent with acute liver disease, and microcystin-LR was present at 3000 μg/L in a reservoir water sample and at 7100 μg/L in the rumen contents of one of the mortalities. Serum biochemistry and histological examination indicated severe liver damage consistent with microcystin toxicosis. Microscopic observation of reservoir water samples, limited to frozen or poorly stored and partially degraded samples, indicated the presence of abundant Anabaena/Dolichospermum, but the presence of other toxic cyanobacteria such as Microcystis could not be excluded. Metagenomic analysis showed the presence in these samples of a single cyanobacterium whose cpcBA, rpoB and rbcL genes indicated membership in the Anabaena/Dolichospermum genus. The sequence of a complete mcy gene cluster with homology to previously identified Anabaena mcy genes was recovered. These results emphasize the capacity for Anabaena/Dolichospermum blooms to produce lethal levels of microcystin, posing a danger to public health and livestock. Further, our findings indicate that such occurrences can occur outside the far-northern latitudes in which microcystin-producing Anabaena have typically been found. Elsevier 2018-12-10 /pmc/articles/PMC7286090/ /pubmed/32831346 http://dx.doi.org/10.1016/j.toxcx.2018.100003 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Paper
Dreher, Theo W.
Collart, Lindsay P.
Mueller, Ryan S.
Halsey, Kimberly H.
Bildfell, Robert J.
Schreder, Peter
Sobhakumari, Arya
Ferry, Rodney
Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event
title Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event
title_full Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event
title_fullStr Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event
title_full_unstemmed Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event
title_short Anabaena/Dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event
title_sort anabaena/dolichospermum as the source of lethal microcystin levels responsible for a large cattle toxicosis event
topic Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286090/
https://www.ncbi.nlm.nih.gov/pubmed/32831346
http://dx.doi.org/10.1016/j.toxcx.2018.100003
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