Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway

Various strains of infectious bronchitis virus (IBV) cause different forms of infectious bronchitis with different clinical signs. Here, primary chicken embryo kidney (CEK) cells and specific-pathogen-free (SPF) chickens were infected with three pathogenic IBV strains, and it was observed that the T...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Jinyan, Xu, Shuang, Li, Xueyan, Wang, Jue, Jiang, Yueqi, Hu, Weichen, Ruan, Wenke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286419/
https://www.ncbi.nlm.nih.gov/pubmed/32524263
http://dx.doi.org/10.1007/s00705-020-04690-8
_version_ 1783544874359848960
author Zhu, Jinyan
Xu, Shuang
Li, Xueyan
Wang, Jue
Jiang, Yueqi
Hu, Weichen
Ruan, Wenke
author_facet Zhu, Jinyan
Xu, Shuang
Li, Xueyan
Wang, Jue
Jiang, Yueqi
Hu, Weichen
Ruan, Wenke
author_sort Zhu, Jinyan
collection PubMed
description Various strains of infectious bronchitis virus (IBV) cause different forms of infectious bronchitis with different clinical signs. Here, primary chicken embryo kidney (CEK) cells and specific-pathogen-free (SPF) chickens were infected with three pathogenic IBV strains, and it was observed that the TLR7-MYD88 pathway was inhibited but the TLR3-TIRF pathway was activated. After treatment with poly(I:C)-LMW, poly (I:C)-LMW/LyoVec, and Imiquimod, the replication of IBV was significantly suppressed after 24 h. However, treatment with TLR3 pathway inhibitors such as Pepinh-TRIF, celastrol, chloroquine, and BX795 resulted in increased replication of IBV after 36 h. These results also showed that chloroquine and celastrol were most effective inhibitors of the antiviral response at 48 hpi.
format Online
Article
Text
id pubmed-7286419
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-72864192020-06-11 Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway Zhu, Jinyan Xu, Shuang Li, Xueyan Wang, Jue Jiang, Yueqi Hu, Weichen Ruan, Wenke Arch Virol Brief Report Various strains of infectious bronchitis virus (IBV) cause different forms of infectious bronchitis with different clinical signs. Here, primary chicken embryo kidney (CEK) cells and specific-pathogen-free (SPF) chickens were infected with three pathogenic IBV strains, and it was observed that the TLR7-MYD88 pathway was inhibited but the TLR3-TIRF pathway was activated. After treatment with poly(I:C)-LMW, poly (I:C)-LMW/LyoVec, and Imiquimod, the replication of IBV was significantly suppressed after 24 h. However, treatment with TLR3 pathway inhibitors such as Pepinh-TRIF, celastrol, chloroquine, and BX795 resulted in increased replication of IBV after 36 h. These results also showed that chloroquine and celastrol were most effective inhibitors of the antiviral response at 48 hpi. Springer Vienna 2020-06-10 2020 /pmc/articles/PMC7286419/ /pubmed/32524263 http://dx.doi.org/10.1007/s00705-020-04690-8 Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Zhu, Jinyan
Xu, Shuang
Li, Xueyan
Wang, Jue
Jiang, Yueqi
Hu, Weichen
Ruan, Wenke
Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway
title Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway
title_full Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway
title_fullStr Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway
title_full_unstemmed Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway
title_short Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway
title_sort infectious bronchitis virus inhibits activation of the tlr7 pathway, but not the tlr3 pathway
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286419/
https://www.ncbi.nlm.nih.gov/pubmed/32524263
http://dx.doi.org/10.1007/s00705-020-04690-8
work_keys_str_mv AT zhujinyan infectiousbronchitisvirusinhibitsactivationofthetlr7pathwaybutnotthetlr3pathway
AT xushuang infectiousbronchitisvirusinhibitsactivationofthetlr7pathwaybutnotthetlr3pathway
AT lixueyan infectiousbronchitisvirusinhibitsactivationofthetlr7pathwaybutnotthetlr3pathway
AT wangjue infectiousbronchitisvirusinhibitsactivationofthetlr7pathwaybutnotthetlr3pathway
AT jiangyueqi infectiousbronchitisvirusinhibitsactivationofthetlr7pathwaybutnotthetlr3pathway
AT huweichen infectiousbronchitisvirusinhibitsactivationofthetlr7pathwaybutnotthetlr3pathway
AT ruanwenke infectiousbronchitisvirusinhibitsactivationofthetlr7pathwaybutnotthetlr3pathway

Ejemplares similares