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Transgenesis and web resources in quail

Due to its amenability to manipulations, to live observation and its striking similarities to mammals, the chicken embryo has been one of the major animal models in biomedical research. Although it is technically possible to genome-edit the chicken, its long generation time (6 months to sexual matur...

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Autores principales: Serralbo, Olivier, Salgado, David, Véron, Nadège, Cooper, Caitlin, Dejardin, Marie-Julie, Doran, Timothy, Gros, Jérome, Marcelle, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286689/
https://www.ncbi.nlm.nih.gov/pubmed/32459172
http://dx.doi.org/10.7554/eLife.56312
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author Serralbo, Olivier
Salgado, David
Véron, Nadège
Cooper, Caitlin
Dejardin, Marie-Julie
Doran, Timothy
Gros, Jérome
Marcelle, Christophe
author_facet Serralbo, Olivier
Salgado, David
Véron, Nadège
Cooper, Caitlin
Dejardin, Marie-Julie
Doran, Timothy
Gros, Jérome
Marcelle, Christophe
author_sort Serralbo, Olivier
collection PubMed
description Due to its amenability to manipulations, to live observation and its striking similarities to mammals, the chicken embryo has been one of the major animal models in biomedical research. Although it is technically possible to genome-edit the chicken, its long generation time (6 months to sexual maturity) makes it an impractical lab model and has prevented it widespread use in research. The Japanese quail (Coturnix coturnix japonica) is an attractive alternative, very similar to the chicken, but with the decisive asset of a much shorter generation time (1.5 months). In recent years, transgenic quail lines have been described. Most of them were generated using replication-deficient lentiviruses, a technique that presents diverse limitations. Here, we introduce a novel technology to perform transgenesis in quail, based on the in vivo transfection of plasmids in circulating Primordial Germ Cells (PGCs). This technique is simple, efficient and allows using the infinite variety of genome engineering approaches developed in other models. Furthermore, we present a website centralizing quail genomic and technological information to facilitate the design of genome-editing strategies, showcase the past and future transgenic quail lines and foster collaborative work within the avian community.
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spelling pubmed-72866892020-06-11 Transgenesis and web resources in quail Serralbo, Olivier Salgado, David Véron, Nadège Cooper, Caitlin Dejardin, Marie-Julie Doran, Timothy Gros, Jérome Marcelle, Christophe eLife Genetics and Genomics Due to its amenability to manipulations, to live observation and its striking similarities to mammals, the chicken embryo has been one of the major animal models in biomedical research. Although it is technically possible to genome-edit the chicken, its long generation time (6 months to sexual maturity) makes it an impractical lab model and has prevented it widespread use in research. The Japanese quail (Coturnix coturnix japonica) is an attractive alternative, very similar to the chicken, but with the decisive asset of a much shorter generation time (1.5 months). In recent years, transgenic quail lines have been described. Most of them were generated using replication-deficient lentiviruses, a technique that presents diverse limitations. Here, we introduce a novel technology to perform transgenesis in quail, based on the in vivo transfection of plasmids in circulating Primordial Germ Cells (PGCs). This technique is simple, efficient and allows using the infinite variety of genome engineering approaches developed in other models. Furthermore, we present a website centralizing quail genomic and technological information to facilitate the design of genome-editing strategies, showcase the past and future transgenic quail lines and foster collaborative work within the avian community. eLife Sciences Publications, Ltd 2020-05-27 /pmc/articles/PMC7286689/ /pubmed/32459172 http://dx.doi.org/10.7554/eLife.56312 Text en © 2020, Serralbo et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Genetics and Genomics
Serralbo, Olivier
Salgado, David
Véron, Nadège
Cooper, Caitlin
Dejardin, Marie-Julie
Doran, Timothy
Gros, Jérome
Marcelle, Christophe
Transgenesis and web resources in quail
title Transgenesis and web resources in quail
title_full Transgenesis and web resources in quail
title_fullStr Transgenesis and web resources in quail
title_full_unstemmed Transgenesis and web resources in quail
title_short Transgenesis and web resources in quail
title_sort transgenesis and web resources in quail
topic Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286689/
https://www.ncbi.nlm.nih.gov/pubmed/32459172
http://dx.doi.org/10.7554/eLife.56312
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