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CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks

Type V CRISPR-Cas interference proteins use a single RuvC active site to make RNA-guided breaks in double-stranded DNA substrates, an activity essential for both bacterial immunity and genome editing. The best-studied of these enzymes, Cas12a, initiates DNA cutting by forming a 20-nucleotide R-loop...

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Detalles Bibliográficos
Autores principales:	Cofsky, Joshua C, Karandur, Deepti, Huang, Carolyn J, Witte, Isaac P, Kuriyan, John, Doudna, Jennifer A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2020
Materias:	Biochemistry and Chemical Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286691/ https://www.ncbi.nlm.nih.gov/pubmed/32519675 http://dx.doi.org/10.7554/eLife.55143

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