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Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum

Fluorescence-based assays should be feasible in aqueous media for effectively detecting the biological factors. However, numerous sensors have limited signal transductions and low fluorescence quantum yields due to the ingerently reduced excited state energy of fluorophores in aqueous solution, whic...

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Detalles Bibliográficos
Autores principales: Jang, Yeon Jin, Kim, Boyun, Roh, Euijin, Kim, Hyunuk, Lee, Seoung Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287074/
https://www.ncbi.nlm.nih.gov/pubmed/32523015
http://dx.doi.org/10.1038/s41598-020-66360-8
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author Jang, Yeon Jin
Kim, Boyun
Roh, Euijin
Kim, Hyunuk
Lee, Seoung Ho
author_facet Jang, Yeon Jin
Kim, Boyun
Roh, Euijin
Kim, Hyunuk
Lee, Seoung Ho
author_sort Jang, Yeon Jin
collection PubMed
description Fluorescence-based assays should be feasible in aqueous media for effectively detecting the biological factors. However, numerous sensors have limited signal transductions and low fluorescence quantum yields due to the ingerently reduced excited state energy of fluorophores in aqueous solution, which reduces their sensitivity. This necessitates a smart sensing approach with an amplified fluorescence response for analytes in aqueous solution. Herein, a new building block which self-assembles in aqueous media, giving a micellar sturcuture with the hydrophobic π-extended conjugated system at the core and hydrophilic groups at the periphery, was devised for the first time. We demonstrated that the aggregated fluorophores in a micelle induce amplified fluorescence quenching, in which the excited electron efficiently migrates through π-extended conjugated system in a micelle, as in a polymeric system. Such feature differentiates this sensing approach from the numerous fluorescence-based tools previously developed for sensitive detection. This new system exhibited highly sensitive signal transduction for specific analytes even under actual bioanalytical conditions.
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spelling pubmed-72870742020-06-15 Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum Jang, Yeon Jin Kim, Boyun Roh, Euijin Kim, Hyunuk Lee, Seoung Ho Sci Rep Article Fluorescence-based assays should be feasible in aqueous media for effectively detecting the biological factors. However, numerous sensors have limited signal transductions and low fluorescence quantum yields due to the ingerently reduced excited state energy of fluorophores in aqueous solution, which reduces their sensitivity. This necessitates a smart sensing approach with an amplified fluorescence response for analytes in aqueous solution. Herein, a new building block which self-assembles in aqueous media, giving a micellar sturcuture with the hydrophobic π-extended conjugated system at the core and hydrophilic groups at the periphery, was devised for the first time. We demonstrated that the aggregated fluorophores in a micelle induce amplified fluorescence quenching, in which the excited electron efficiently migrates through π-extended conjugated system in a micelle, as in a polymeric system. Such feature differentiates this sensing approach from the numerous fluorescence-based tools previously developed for sensitive detection. This new system exhibited highly sensitive signal transduction for specific analytes even under actual bioanalytical conditions. Nature Publishing Group UK 2020-06-10 /pmc/articles/PMC7287074/ /pubmed/32523015 http://dx.doi.org/10.1038/s41598-020-66360-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Jang, Yeon Jin
Kim, Boyun
Roh, Euijin
Kim, Hyunuk
Lee, Seoung Ho
Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum
title Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum
title_full Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum
title_fullStr Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum
title_full_unstemmed Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum
title_short Micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum
title_sort micellization-induced amplified fluorescence response for highly sensitive detection of heparin in serum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287074/
https://www.ncbi.nlm.nih.gov/pubmed/32523015
http://dx.doi.org/10.1038/s41598-020-66360-8
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