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Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs
Rapeseed meal is a sustainable feed ingredient that can be used as an alternative to imported soybean meal in European pig production. The gut microbiota plays an important role on pig physiology and health but the impact on microbiota of using rapeseed in diets is still not well known. In this stud...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287078/ https://www.ncbi.nlm.nih.gov/pubmed/32523033 http://dx.doi.org/10.1038/s41598-020-66364-4 |
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author | Umu, Özgün Candan Onarman Mydland, Liv Torunn Øverland, Margareth Press, Charles McLean Sørum, Henning |
author_facet | Umu, Özgün Candan Onarman Mydland, Liv Torunn Øverland, Margareth Press, Charles McLean Sørum, Henning |
author_sort | Umu, Özgün Candan Onarman |
collection | PubMed |
description | Rapeseed meal is a sustainable feed ingredient that can be used as an alternative to imported soybean meal in European pig production. The gut microbiota plays an important role on pig physiology and health but the impact on microbiota of using rapeseed in diets is still not well known. In this study, 84 purebred Norwegian Landrace pigs with average initial weight of 25 kg were divided into two groups and fed for approximately three months with either a control diet containing soybean meal (CON) or a high-fiber experimental diet where 20% rapeseed meal (RSF) was included as an alternative to soybean meal in CON. The composition and function of microbiome in gut digesta samples were analyzed by performing 16S rRNA gene sequencing and culturing of bacteria. The microbiota diversity and composition were similar between the dietary treatments; however, relative abundance of a variety of bacterial groups and imputed functions of microbiome in the ileum and large intestine were altered when the pigs were fed with a rapeseed-based diet. It was notable that the immune-inducing bacterial group Mucispirillum and anti-inflammatory stimulating bacteria Lachnospira were more abundant in the ileum and large intestine of the RSF group, respectively. Moreover, there was a higher abundance of major amino acid fermenters and amylolytic bacteria in the CON group and a high abundance of putative short chain fatty acid producers in RSF group. In comparison with the CON group, the gut microbiome of RSF group possessed an enhanced potential for carbohydrate and energy metabolism and a reduced potential for bacterial pathogenicity-related pathways. |
format | Online Article Text |
id | pubmed-7287078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72870782020-06-15 Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs Umu, Özgün Candan Onarman Mydland, Liv Torunn Øverland, Margareth Press, Charles McLean Sørum, Henning Sci Rep Article Rapeseed meal is a sustainable feed ingredient that can be used as an alternative to imported soybean meal in European pig production. The gut microbiota plays an important role on pig physiology and health but the impact on microbiota of using rapeseed in diets is still not well known. In this study, 84 purebred Norwegian Landrace pigs with average initial weight of 25 kg were divided into two groups and fed for approximately three months with either a control diet containing soybean meal (CON) or a high-fiber experimental diet where 20% rapeseed meal (RSF) was included as an alternative to soybean meal in CON. The composition and function of microbiome in gut digesta samples were analyzed by performing 16S rRNA gene sequencing and culturing of bacteria. The microbiota diversity and composition were similar between the dietary treatments; however, relative abundance of a variety of bacterial groups and imputed functions of microbiome in the ileum and large intestine were altered when the pigs were fed with a rapeseed-based diet. It was notable that the immune-inducing bacterial group Mucispirillum and anti-inflammatory stimulating bacteria Lachnospira were more abundant in the ileum and large intestine of the RSF group, respectively. Moreover, there was a higher abundance of major amino acid fermenters and amylolytic bacteria in the CON group and a high abundance of putative short chain fatty acid producers in RSF group. In comparison with the CON group, the gut microbiome of RSF group possessed an enhanced potential for carbohydrate and energy metabolism and a reduced potential for bacterial pathogenicity-related pathways. Nature Publishing Group UK 2020-06-10 /pmc/articles/PMC7287078/ /pubmed/32523033 http://dx.doi.org/10.1038/s41598-020-66364-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Umu, Özgün Candan Onarman Mydland, Liv Torunn Øverland, Margareth Press, Charles McLean Sørum, Henning Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs |
title | Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs |
title_full | Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs |
title_fullStr | Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs |
title_full_unstemmed | Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs |
title_short | Rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs |
title_sort | rapeseed-based diet modulates the imputed functions of gut microbiome in growing-finishing pigs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287078/ https://www.ncbi.nlm.nih.gov/pubmed/32523033 http://dx.doi.org/10.1038/s41598-020-66364-4 |
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