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Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay

β2-Glycoprotein I (β2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/β2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both sy...

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Autores principales: Tan, Xian Wen, Takenaka, Fumiaki, Takekawa, Hironori, Mastuura, Eiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287255/
https://www.ncbi.nlm.nih.gov/pubmed/32551380
http://dx.doi.org/10.1016/j.heliyon.2020.e04114
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author Tan, Xian Wen
Takenaka, Fumiaki
Takekawa, Hironori
Mastuura, Eiji
author_facet Tan, Xian Wen
Takenaka, Fumiaki
Takekawa, Hironori
Mastuura, Eiji
author_sort Tan, Xian Wen
collection PubMed
description β2-Glycoprotein I (β2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/β2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both systemic and non-systemic autoimmune diseases. Hence, serological level of oxLDL/β2GPI complexes may represent one crucial clinical parameter for disease prognosis of atherosclerosis-related diseases. Herein, we established a simple, specific and rapid gold nanoparticle (GNP) based lateral flow immunoassay (LFIA) to quantify oxLDL/β2GPI complexes from test samples. Specificities of hybridoma cell-derived monoclonal antibodies against antigen, optimal conditions for conjugation of antibody with GNP, and sensitivity of oxLDL/β2GPI LFIA in comparison to an ELISA-based detection method were assessed accordingly. The established oxLDL/β2GPI LFIA was capable of detecting oxLDL/β2GPI specifically without interference from autoantibodies and solitary components of oxLDL/β2GPI present in test samples. A significant correlation (R(2) > 0.8) was also obtained with the oxLDL/β2GPI LFIA when compared to the ELISA-based detection. On the whole, the oxLDL/β2GPI LFIA remains advantageous over the oxLDL/β2GPI ELISA. The unnecessary washing step, short developmental and analytical time support facile and rapid detection of oxLDL/β2GPI as opposed to the laborious ELISA system.
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spelling pubmed-72872552020-06-17 Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay Tan, Xian Wen Takenaka, Fumiaki Takekawa, Hironori Mastuura, Eiji Heliyon Article β2-Glycoprotein I (β2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/β2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both systemic and non-systemic autoimmune diseases. Hence, serological level of oxLDL/β2GPI complexes may represent one crucial clinical parameter for disease prognosis of atherosclerosis-related diseases. Herein, we established a simple, specific and rapid gold nanoparticle (GNP) based lateral flow immunoassay (LFIA) to quantify oxLDL/β2GPI complexes from test samples. Specificities of hybridoma cell-derived monoclonal antibodies against antigen, optimal conditions for conjugation of antibody with GNP, and sensitivity of oxLDL/β2GPI LFIA in comparison to an ELISA-based detection method were assessed accordingly. The established oxLDL/β2GPI LFIA was capable of detecting oxLDL/β2GPI specifically without interference from autoantibodies and solitary components of oxLDL/β2GPI present in test samples. A significant correlation (R(2) > 0.8) was also obtained with the oxLDL/β2GPI LFIA when compared to the ELISA-based detection. On the whole, the oxLDL/β2GPI LFIA remains advantageous over the oxLDL/β2GPI ELISA. The unnecessary washing step, short developmental and analytical time support facile and rapid detection of oxLDL/β2GPI as opposed to the laborious ELISA system. Elsevier 2020-06-08 /pmc/articles/PMC7287255/ /pubmed/32551380 http://dx.doi.org/10.1016/j.heliyon.2020.e04114 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Tan, Xian Wen
Takenaka, Fumiaki
Takekawa, Hironori
Mastuura, Eiji
Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
title Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
title_full Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
title_fullStr Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
title_full_unstemmed Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
title_short Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
title_sort rapid and specific detection of oxidized ldl/β2gpi complexes via facile lateral flow immunoassay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287255/
https://www.ncbi.nlm.nih.gov/pubmed/32551380
http://dx.doi.org/10.1016/j.heliyon.2020.e04114
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