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Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay
β2-Glycoprotein I (β2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/β2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both sy...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287255/ https://www.ncbi.nlm.nih.gov/pubmed/32551380 http://dx.doi.org/10.1016/j.heliyon.2020.e04114 |
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author | Tan, Xian Wen Takenaka, Fumiaki Takekawa, Hironori Mastuura, Eiji |
author_facet | Tan, Xian Wen Takenaka, Fumiaki Takekawa, Hironori Mastuura, Eiji |
author_sort | Tan, Xian Wen |
collection | PubMed |
description | β2-Glycoprotein I (β2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/β2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both systemic and non-systemic autoimmune diseases. Hence, serological level of oxLDL/β2GPI complexes may represent one crucial clinical parameter for disease prognosis of atherosclerosis-related diseases. Herein, we established a simple, specific and rapid gold nanoparticle (GNP) based lateral flow immunoassay (LFIA) to quantify oxLDL/β2GPI complexes from test samples. Specificities of hybridoma cell-derived monoclonal antibodies against antigen, optimal conditions for conjugation of antibody with GNP, and sensitivity of oxLDL/β2GPI LFIA in comparison to an ELISA-based detection method were assessed accordingly. The established oxLDL/β2GPI LFIA was capable of detecting oxLDL/β2GPI specifically without interference from autoantibodies and solitary components of oxLDL/β2GPI present in test samples. A significant correlation (R(2) > 0.8) was also obtained with the oxLDL/β2GPI LFIA when compared to the ELISA-based detection. On the whole, the oxLDL/β2GPI LFIA remains advantageous over the oxLDL/β2GPI ELISA. The unnecessary washing step, short developmental and analytical time support facile and rapid detection of oxLDL/β2GPI as opposed to the laborious ELISA system. |
format | Online Article Text |
id | pubmed-7287255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-72872552020-06-17 Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay Tan, Xian Wen Takenaka, Fumiaki Takekawa, Hironori Mastuura, Eiji Heliyon Article β2-Glycoprotein I (β2GPI) forms indissociable complex with oxidized LDL (oxLDL) into proatherogenic oxLDL/β2GPI complex through a specific ligand known as 7-ketocholesteryl-9-carboxynonanoate (oxLig-1). Recent discoveries have demonstrated the atherogenicity of these complexes in patients of both systemic and non-systemic autoimmune diseases. Hence, serological level of oxLDL/β2GPI complexes may represent one crucial clinical parameter for disease prognosis of atherosclerosis-related diseases. Herein, we established a simple, specific and rapid gold nanoparticle (GNP) based lateral flow immunoassay (LFIA) to quantify oxLDL/β2GPI complexes from test samples. Specificities of hybridoma cell-derived monoclonal antibodies against antigen, optimal conditions for conjugation of antibody with GNP, and sensitivity of oxLDL/β2GPI LFIA in comparison to an ELISA-based detection method were assessed accordingly. The established oxLDL/β2GPI LFIA was capable of detecting oxLDL/β2GPI specifically without interference from autoantibodies and solitary components of oxLDL/β2GPI present in test samples. A significant correlation (R(2) > 0.8) was also obtained with the oxLDL/β2GPI LFIA when compared to the ELISA-based detection. On the whole, the oxLDL/β2GPI LFIA remains advantageous over the oxLDL/β2GPI ELISA. The unnecessary washing step, short developmental and analytical time support facile and rapid detection of oxLDL/β2GPI as opposed to the laborious ELISA system. Elsevier 2020-06-08 /pmc/articles/PMC7287255/ /pubmed/32551380 http://dx.doi.org/10.1016/j.heliyon.2020.e04114 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Tan, Xian Wen Takenaka, Fumiaki Takekawa, Hironori Mastuura, Eiji Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay |
title | Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay |
title_full | Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay |
title_fullStr | Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay |
title_full_unstemmed | Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay |
title_short | Rapid and specific detection of oxidized LDL/β2GPI complexes via facile lateral flow immunoassay |
title_sort | rapid and specific detection of oxidized ldl/β2gpi complexes via facile lateral flow immunoassay |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287255/ https://www.ncbi.nlm.nih.gov/pubmed/32551380 http://dx.doi.org/10.1016/j.heliyon.2020.e04114 |
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