Cargando…

First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid

Coronavirus disease 2019 (COVID-19) is a pandemic infection due to the spread of a novel coronavirus (severe acute respiratory syndrome coronavirus 2), resulting in a wide range of clinical features, from asymptomatic carriers to ARDS. The gold standard for diagnosis is nucleic acid detection by rea...

Descripción completa

Detalles Bibliográficos
Autores principales: Mei, Federico, Bonifazi, Martina, Menzo, Stefano, Di Marco Berardino, Alessandro, Sediari, Michele, Paolini, Luca, Re, Antonina, Gonnelli, Francesca, Duranti, Claudia, Grilli, Martina, Vennarucci, Giacomo Spurio, Latini, Maria Agnese, Zuccatosta, Lina, Gasparini, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American College of Chest Physicians. Published by Elsevier Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287448/
https://www.ncbi.nlm.nih.gov/pubmed/32534909
http://dx.doi.org/10.1016/j.chest.2020.05.583
_version_ 1783545064842067968
author Mei, Federico
Bonifazi, Martina
Menzo, Stefano
Di Marco Berardino, Alessandro
Sediari, Michele
Paolini, Luca
Re, Antonina
Gonnelli, Francesca
Duranti, Claudia
Grilli, Martina
Vennarucci, Giacomo Spurio
Latini, Maria Agnese
Zuccatosta, Lina
Gasparini, Stefano
author_facet Mei, Federico
Bonifazi, Martina
Menzo, Stefano
Di Marco Berardino, Alessandro
Sediari, Michele
Paolini, Luca
Re, Antonina
Gonnelli, Francesca
Duranti, Claudia
Grilli, Martina
Vennarucci, Giacomo Spurio
Latini, Maria Agnese
Zuccatosta, Lina
Gasparini, Stefano
author_sort Mei, Federico
collection PubMed
description Coronavirus disease 2019 (COVID-19) is a pandemic infection due to the spread of a novel coronavirus (severe acute respiratory syndrome coronavirus 2), resulting in a wide range of clinical features, from asymptomatic carriers to ARDS. The gold standard for diagnosis is nucleic acid detection by real-time reverse transcriptase-polymerase chain reaction in nasopharyngeal swabs. However, due to limitations in this technique’s sensitivity, thoracic imaging plays a crucial, complementary role in diagnostic evaluation and also allows for detection of atypical findings and potential alternative targets for sampling (eg, pleural effusion). Although less common, pleural involvement has been described in a minority of patients. This report describes the first case of reverse transcriptase-polymerase chain reaction detection of severe acute respiratory syndrome coronavirus 2 in pleural fluid obtained by means of ultrasound-guided thoracentesis, and its main characteristics are detailed. Pleural effusion is not a common finding in COVID-19 infection, but a prompt recognition of this potential localization may be useful to optimize diagnostic evaluation as well as the management of these patients.
format Online
Article
Text
id pubmed-7287448
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher American College of Chest Physicians. Published by Elsevier Inc.
record_format MEDLINE/PubMed
spelling pubmed-72874482020-06-11 First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid Mei, Federico Bonifazi, Martina Menzo, Stefano Di Marco Berardino, Alessandro Sediari, Michele Paolini, Luca Re, Antonina Gonnelli, Francesca Duranti, Claudia Grilli, Martina Vennarucci, Giacomo Spurio Latini, Maria Agnese Zuccatosta, Lina Gasparini, Stefano Chest Novel Reports Coronavirus disease 2019 (COVID-19) is a pandemic infection due to the spread of a novel coronavirus (severe acute respiratory syndrome coronavirus 2), resulting in a wide range of clinical features, from asymptomatic carriers to ARDS. The gold standard for diagnosis is nucleic acid detection by real-time reverse transcriptase-polymerase chain reaction in nasopharyngeal swabs. However, due to limitations in this technique’s sensitivity, thoracic imaging plays a crucial, complementary role in diagnostic evaluation and also allows for detection of atypical findings and potential alternative targets for sampling (eg, pleural effusion). Although less common, pleural involvement has been described in a minority of patients. This report describes the first case of reverse transcriptase-polymerase chain reaction detection of severe acute respiratory syndrome coronavirus 2 in pleural fluid obtained by means of ultrasound-guided thoracentesis, and its main characteristics are detailed. Pleural effusion is not a common finding in COVID-19 infection, but a prompt recognition of this potential localization may be useful to optimize diagnostic evaluation as well as the management of these patients. American College of Chest Physicians. Published by Elsevier Inc. 2020-10 2020-06-11 /pmc/articles/PMC7287448/ /pubmed/32534909 http://dx.doi.org/10.1016/j.chest.2020.05.583 Text en © 2020 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Novel Reports
Mei, Federico
Bonifazi, Martina
Menzo, Stefano
Di Marco Berardino, Alessandro
Sediari, Michele
Paolini, Luca
Re, Antonina
Gonnelli, Francesca
Duranti, Claudia
Grilli, Martina
Vennarucci, Giacomo Spurio
Latini, Maria Agnese
Zuccatosta, Lina
Gasparini, Stefano
First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
title First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
title_full First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
title_fullStr First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
title_full_unstemmed First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
title_short First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid
title_sort first detection of sars-cov-2 by real-time reverse transcriptase-polymerase chain reaction assay in pleural fluid
topic Novel Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287448/
https://www.ncbi.nlm.nih.gov/pubmed/32534909
http://dx.doi.org/10.1016/j.chest.2020.05.583
work_keys_str_mv AT meifederico firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT bonifazimartina firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT menzostefano firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT dimarcoberardinoalessandro firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT sediarimichele firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT paoliniluca firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT reantonina firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT gonnellifrancesca firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT duranticlaudia firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT grillimartina firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT vennaruccigiacomospurio firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT latinimariaagnese firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT zuccatostalina firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid
AT gasparinistefano firstdetectionofsarscov2byrealtimereversetranscriptasepolymerasechainreactionassayinpleuralfluid