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The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish

The mosquito-borne viruses dengue (DENV) and Zika (ZIKV) viruses are two medically important pathogens in tropical and subtropical regions of the world. There is an urgent need of therapeutics against DENV and ZIKV, and medicinal plants are considered as a promising source of antiviral bioactive met...

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Autores principales: Haddad, Juliano G., Grauzdytė, Dovilė, Koishi, Andrea Cristine, Viranaicken, Wildriss, Venskutonis, Petras Rimantas, Nunes Duarte dos Santos, Claudia, Desprès, Philippe, Diotel, Nicolas, El Kalamouni, Chaker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287739/
https://www.ncbi.nlm.nih.gov/pubmed/32429073
http://dx.doi.org/10.3390/molecules25102316
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author Haddad, Juliano G.
Grauzdytė, Dovilė
Koishi, Andrea Cristine
Viranaicken, Wildriss
Venskutonis, Petras Rimantas
Nunes Duarte dos Santos, Claudia
Desprès, Philippe
Diotel, Nicolas
El Kalamouni, Chaker
author_facet Haddad, Juliano G.
Grauzdytė, Dovilė
Koishi, Andrea Cristine
Viranaicken, Wildriss
Venskutonis, Petras Rimantas
Nunes Duarte dos Santos, Claudia
Desprès, Philippe
Diotel, Nicolas
El Kalamouni, Chaker
author_sort Haddad, Juliano G.
collection PubMed
description The mosquito-borne viruses dengue (DENV) and Zika (ZIKV) viruses are two medically important pathogens in tropical and subtropical regions of the world. There is an urgent need of therapeutics against DENV and ZIKV, and medicinal plants are considered as a promising source of antiviral bioactive metabolites. In the present study, we evaluated the ability of Phyllanthus phillyreifolius, an endemic medicinal plant from Reunion Island, to prevent DENV and ZIKV infection in human cells. At non-cytotoxic concentration in vitro, incubation of infected A549 cells with a P. phillyreifolius extract or its major active phytochemical geraniin resulted in a dramatic reduction of virus progeny production for ZIKV as well as four serotypes of DENV. Virological assays showed that P. phillyreifolius extract-mediated virus inhibition relates to a blockade in internalization of virus particles into the host cell. Infectivity studies on ZIKV showed that both P. phillyreifolius and geraniin cause a loss of infectivity of the viral particles. Using a zebrafish model, we demonstrated that administration of P. phillyreifolius and geraniin has no effect on zebrafish locomotor activity while no morbidity nor mortality was observed up to 5 days post-inoculation. Thus, P. phillyreifolius could act as an important source of plant metabolite geraniin which is a promising antiviral compound in the fight against DENV and ZIKV.
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spelling pubmed-72877392020-06-15 The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish Haddad, Juliano G. Grauzdytė, Dovilė Koishi, Andrea Cristine Viranaicken, Wildriss Venskutonis, Petras Rimantas Nunes Duarte dos Santos, Claudia Desprès, Philippe Diotel, Nicolas El Kalamouni, Chaker Molecules Article The mosquito-borne viruses dengue (DENV) and Zika (ZIKV) viruses are two medically important pathogens in tropical and subtropical regions of the world. There is an urgent need of therapeutics against DENV and ZIKV, and medicinal plants are considered as a promising source of antiviral bioactive metabolites. In the present study, we evaluated the ability of Phyllanthus phillyreifolius, an endemic medicinal plant from Reunion Island, to prevent DENV and ZIKV infection in human cells. At non-cytotoxic concentration in vitro, incubation of infected A549 cells with a P. phillyreifolius extract or its major active phytochemical geraniin resulted in a dramatic reduction of virus progeny production for ZIKV as well as four serotypes of DENV. Virological assays showed that P. phillyreifolius extract-mediated virus inhibition relates to a blockade in internalization of virus particles into the host cell. Infectivity studies on ZIKV showed that both P. phillyreifolius and geraniin cause a loss of infectivity of the viral particles. Using a zebrafish model, we demonstrated that administration of P. phillyreifolius and geraniin has no effect on zebrafish locomotor activity while no morbidity nor mortality was observed up to 5 days post-inoculation. Thus, P. phillyreifolius could act as an important source of plant metabolite geraniin which is a promising antiviral compound in the fight against DENV and ZIKV. MDPI 2020-05-15 /pmc/articles/PMC7287739/ /pubmed/32429073 http://dx.doi.org/10.3390/molecules25102316 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Haddad, Juliano G.
Grauzdytė, Dovilė
Koishi, Andrea Cristine
Viranaicken, Wildriss
Venskutonis, Petras Rimantas
Nunes Duarte dos Santos, Claudia
Desprès, Philippe
Diotel, Nicolas
El Kalamouni, Chaker
The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish
title The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish
title_full The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish
title_fullStr The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish
title_full_unstemmed The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish
title_short The Geraniin-Rich Extract from Reunion Island Endemic Medicinal Plant Phyllanthus phillyreifolius Inhibits Zika and Dengue Virus Infection at Non-Toxic Effect Doses in Zebrafish
title_sort geraniin-rich extract from reunion island endemic medicinal plant phyllanthus phillyreifolius inhibits zika and dengue virus infection at non-toxic effect doses in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287739/
https://www.ncbi.nlm.nih.gov/pubmed/32429073
http://dx.doi.org/10.3390/molecules25102316
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