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Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect

The determination of rifampicin in pharmaceutical dosage forms using a rapid, sensitive, selective, biocompatible, and low-cost method is of vital importance in the pharmaceutical analysis field to ensure its concentration is within the effective range when administered. In this study, nitrogen-and-...

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Autores principales: Al-Hashimi, Baraa, Rahman, Heshu Sulaiman, Omer, Khalid Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287754/
https://www.ncbi.nlm.nih.gov/pubmed/32429119
http://dx.doi.org/10.3390/ma13102275
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author Al-Hashimi, Baraa
Rahman, Heshu Sulaiman
Omer, Khalid Mohammad
author_facet Al-Hashimi, Baraa
Rahman, Heshu Sulaiman
Omer, Khalid Mohammad
author_sort Al-Hashimi, Baraa
collection PubMed
description The determination of rifampicin in pharmaceutical dosage forms using a rapid, sensitive, selective, biocompatible, and low-cost method is of vital importance in the pharmaceutical analysis field to ensure its concentration is within the effective range when administered. In this study, nitrogen-and-phosphorous-doped carbon nanodots (CNDs) were prepared using a single-step hydrothermal method with ciprofloxacin as the starting material. The CNDs showed a highly intense blue fluorescence emission centered at 450 nm, with a photoluminescence quantum yield of about 51%. Since the absorption of rifampicin was the same as the excitation spectrum of CNDs, inner filter effect (IFE) quenching occurred and it was used as a successful detection platform for the analysis of rifampicin in capsules. The detection platform showed a dynamic linear range from 1 to 100 μM (R(2) = 0.9940) and the limit of detection was 0.06 μM (when S/N = 3). The average spike recovery percentage for rifampicin in the capsule samples was 100.53% (n = 5). Moreover, the sub-chronic cytotoxicity of CNDs was evaluated on healthy male mice (Balb/c) drenched with different amounts of CNDs (10 and 50 mg/kg). During this study period, no mortalities or toxicity signs were recorded in any of the experimental subjects. Based on the cytotoxicity experiment, the proposed nano-probe is considered safe and biocompatible.
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spelling pubmed-72877542020-06-15 Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect Al-Hashimi, Baraa Rahman, Heshu Sulaiman Omer, Khalid Mohammad Materials (Basel) Article The determination of rifampicin in pharmaceutical dosage forms using a rapid, sensitive, selective, biocompatible, and low-cost method is of vital importance in the pharmaceutical analysis field to ensure its concentration is within the effective range when administered. In this study, nitrogen-and-phosphorous-doped carbon nanodots (CNDs) were prepared using a single-step hydrothermal method with ciprofloxacin as the starting material. The CNDs showed a highly intense blue fluorescence emission centered at 450 nm, with a photoluminescence quantum yield of about 51%. Since the absorption of rifampicin was the same as the excitation spectrum of CNDs, inner filter effect (IFE) quenching occurred and it was used as a successful detection platform for the analysis of rifampicin in capsules. The detection platform showed a dynamic linear range from 1 to 100 μM (R(2) = 0.9940) and the limit of detection was 0.06 μM (when S/N = 3). The average spike recovery percentage for rifampicin in the capsule samples was 100.53% (n = 5). Moreover, the sub-chronic cytotoxicity of CNDs was evaluated on healthy male mice (Balb/c) drenched with different amounts of CNDs (10 and 50 mg/kg). During this study period, no mortalities or toxicity signs were recorded in any of the experimental subjects. Based on the cytotoxicity experiment, the proposed nano-probe is considered safe and biocompatible. MDPI 2020-05-15 /pmc/articles/PMC7287754/ /pubmed/32429119 http://dx.doi.org/10.3390/ma13102275 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Hashimi, Baraa
Rahman, Heshu Sulaiman
Omer, Khalid Mohammad
Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect
title Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect
title_full Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect
title_fullStr Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect
title_full_unstemmed Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect
title_short Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect
title_sort highly luminescent and biocompatible p and n co-doped passivated carbon nanodots for the sensitive and selective determination of rifampicin using the inner filter effect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287754/
https://www.ncbi.nlm.nih.gov/pubmed/32429119
http://dx.doi.org/10.3390/ma13102275
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